ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01113.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTThe activity of transglutaminase per unit of wet weight, dry weight, soluble protein, and DNA was determined in three fractions (stratum corneum, malpighii, and dermis) of cow snout skin. The stratum corneum showed the highest enzyme activity among these three fractions.The activity of transglutaminase in the stratum malpighii was ehhanced remarkably by a 36 hour preincubation period at pH 4.5, but the activity in the stratum corneum did not show any increase in enzyme activity under acidic conditions. This ehhancement of enzyme activity was inhibited by pepstatin (100μg/ml) and chymostatin (100μg/ml).Relatively high activities of lysosomal enzymes such asβ‐glucuronidase, cathepsin B1, and cathepsin D were detected in the stratum malpighii fraction, but these enzyme activities were low in the stratum corneum fraction.To further clarify the nature and activation systems of transglutaminase from stratum corneum and malpighii, transglutaminase solutions with the same activity were prepared from the two layers. Then the same amount of cathepsin D in the crude malpighian transglutaminase solution was added in the crude transglutaminase solution of stratum corneum. Following incubation, the transglutaminase activity of stratum corneum became elevated to the same level of enzyme activity as that of the stratum malpighii. These results suggest the importance of cathepsin D in the activation system of transglutaminase and possible activation machanisms of epidermal transglutaminasein vivo. Transglutaminase in the stratum malpighii may exist in a nonactivated form. During the autolytic stages in the stratum granulosum, a small amount of this nonactivated transglutaminase may be activated by released lysosomal acid proteinases such as cathepsin B1and cathepsin D. Thereafter, activated transglutaminase could catalyze the formations ofε‐(γ‐glutamyl) lysine cross‐linking in the proteins synthesized in the cytosol of keratinizing cells with ultimate transformation to the membrane‐lining protein, the so‐called marginal band.In addition, most of the transglutaminase in the stratum corneum may still remain in a nonactivated form, since cathepsin D like enzymes may possibly be inactivated or degraded in the stratu

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01114.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTSeventy‐two strains from species of dermatophytes which had been transferred periodically on Sabouraud's dextrose agar for more than ten years were examined for their morphology, physiological activities and biological properties.Of 42 strains ofTrichophyton rubrum, 17 (40.4%) were positive for pigment production on Sabouraud's dextrose agar. In mating experiments, 15 (79%) of 19 strains tested were compatible withArthroderma simiiand proved to be of the “—” mating type. Eight (67%) of 12 strains in theMicrosporum gypseumcomplex were positive for macroconidium formation. In mating experiments, 4 of 12 strains proved to beNannizzia gypseaof the “+” type, 3 to be of the “—” type, and one to beN. incurvataof the “—” type.Most of the 18 strains in theT. mentagrophytescomplex andM. canishave been main

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01115.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTEffects of chlorpromazine plus UVA‐illumination on RNA were studiedin vitro. Chlorpromazine‐UVA treatment of RNA extracted from bacteriophages MS2 and Qβreduced their infectivity toEscherichia colispheroplasts. Extent of the inactivation was increased with increasing concentrations of chlorpromazine and with increasing fluence of UVA. In the light of our previous findings, chlorpromazine‐adduct to purine residues is suggested as a possible source of damage leading to the inactivation

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01116.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTThe effects of metabolic inhibitors on the outgrowing epidermis were studied to detect the various cellular activities of epidermal cells. Hydroxyurea significantly inhibited mitosis and3H‐thymidine uptake, but the inhibitory effect of hydroxyurea on the rate of epidermal outgrowth was less remarkable. On the other hand, cytochalasin B significantly inhibited the rate of epidermal outgrowth but did not inhibit mitosis or3H‐thymidine uptake. The overall results indicate that there are several cellular activities such as migration and proliferation in the outgrowing epidermisin vi

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01117.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTCutaneous oxygen tension (cutaneous pO2) was measured in 59 healthy controls and 58 patients with peripheral circulatory disturbances including 18 with progressive systemic sclerosis, 5 with stasis dermatitis with ulcer, 22 with arteriosclerosis obliterans, and 13 with thromboangiitis obliterans.In the control group, the mean value ± SD of cutaneous pO2was 82 ± 10 mmHg on the forearm, 79 ± 10 mmHg on the subclavicular region, 81 + 11 mmHg on the leg, 75 ± 11 mmHg on the dorsum of the hand and 70 ± 9 mmHg on the dorsum of the foot. There was an inverse correlation between cutaneous pO2values on the forearm and increase in age, and a significant correlation between cutaneous pO2values on the forearm and femoral arterial oxygen tension (PaO2).In all of the patient groups, cutaneous pO2measured at affected sites was significantly lower than in the control group.These cutaneous pO2values reflect the amount of O2available for diffusion to the skin surface; thus cutaneous pO2measurement is useful as a quantitative evaluation of cutaneous circula

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01118.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTThe SDS‐PAGE patterns of keratinous proteins extracted from whole epidermis and fractionated epidermal cells were studied. Those from the whole and fractionated epidermal cells of psoriatic involved skin showed an extra 58,000 dalton polypeptide in addition to the four major polypeptides (M.W.: 67,000, 63,000, 59,000 and 55,000) which were demonstrated to be common to normal epidermis and psoriatic‐uninvolved epidermis. The stratum corneum of psoriatic involved skin did not contain a 67,000 dalton polypeptide which was present in the stratum corneum of normal and psoriatic uninvolved skin.The presence of the additional 58,000 dalton polypeptide may be due to abnormal synthesis of keratin proteins in the psoriatic epidermis, since this additional polypeptide was present in the lower living layers of the psoriatic epidermis as well. On the other hand, the absence of the 67,000 dalton polypeptide in the psoriatic stratum corneum may be due to enzymatic modifications during the transition from the living cell layers to the horny la

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01119.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTForty‐eight patients were treated using nine potent corticosteroid ointments administered by simple application and their adrenocortical function was examined during treatment. Adrenocortical function was not suppressed by beclomethasone dipropionate, betamethasone valerate, or fluocinonide; was moderately suppressed by diflucortolone valerate, budesonide, and halcinonide; and was markedly suppressed by clobetasol propionate, desoximetasone, and diflorasone diacetate.The dose relationship between topically applied clobetasol propionate ointment and orally administered betamethasone on adrenocortical suppression was also studied. The results suggest that excessive application of very strong topical corticosteroids has much the same effects as systemic administration of small doses of corticosteroid

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01120.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTA forty‐three year old man has had numerous papules on his face and neck for about two years without any systemic involvement. Some papules disappeared gradually within eight months without any therapy. Papules were examined with the light microscope and the electron microscope and histological findings were very similar to those of juvenile xanthogranuloma (JXG)1

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01121.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTA 15 year old Japanese girl, who had suffered from juvenile hypothyroidism noted hypertrichosis and hyperkeratosis on her back and extensor aspects of upper arms. Rolled hairs were seen under a thin transluscent horny layer. These rolled hairs were produced by a follicular plug of keratin which inhibited growing miniature hairs.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01122.x

年代:1983

数据来源: WILEY