ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1991.tb03170.x

年代:1991

数据来源: WILEY

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1991.tb03171.x

年代:1991

数据来源: WILEY

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1991.tb03159.x

年代:1991

数据来源: WILEY

摘要:

AbstractIn the study of the purification of candidacidal cationic proteins from human epidermis by using high performance liquid chromatography, it was found that these proteins were composed of several groups. Among them, the most active group was purified. The amino acid compositions of this protein group were almost the same as those of human splenic H1 histones. The results of immunoblotting suggested that the proteins were human epidermal H1 histones. The killing speed of the epidermal H1 histone was very rapid, 8 μg of the protein killed 90% ofCandida tropicalis(1 times 105CFU) within 10 minutes. The cidal activity increased in lower pH conditions and decreased at higher ionic strengths. Because nuclei of the epidermis disintegrate in the granular layer, it is suggested that, in this layer, nuclear histones may be released from the nuclei. My immunohistochemical results suggest that H1 histones may contribute to form a barrier which inhibits candida from invading deeper than the granular cell layer in cases of skin candidial infection

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1991.tb03160.x

年代:1991

数据来源: WILEY

摘要:

AbstractBimolane, an analog of razoxane has been used in China with comparable efficacy but less toxicity than razoxane in the treatment of psoriasis. In an attempt to characterize further its mode of action it was administered both systemically and topically in theMalassezia ovalisanimal model of psoriasis.Intravenous methotrexate and topical 0.1% betamethasone valerate were also used as positive control treatments.The animal model of psoriasis was effectively treated by bimolane, both systemically and topically, and also by parenteral methotrexate and topical betamethosone valerate.The time course of bimolane's effect with this model was different from methotrexate's suggesting the possibility of a different mode of action.Because bimolane, like razoxane, is an ethylene diamino tetraacetate acid (EDTA) derivative, it is possible that its effects on this reaction relate to its chelating properties and that inhibition of complement activation is important to its mode of action.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1991.tb03161.x

年代:1991

数据来源: WILEY

摘要:

AbstractOne of the most common dermatological side effects of cyclosporin A (CsA) is dose‐dependent hypertrichosis. Similar hair growth was noted in nude mice in an attempt to increase the acceptance of human xenografts with CsA in the T‐cell‐deficient congenitally athymic nude (nu/nu) mice. The aim of the present study was to further investigate the stimulation of hair growth on nude mice not only by oral administration of CsA but also by topical and subcutaneous administration of CsA. Young BALB/c female nude mice were treated for 3 or 4 weeks with topical, oral, or subcutaneous applications of CsA dissolved in olive oil at various doses. The hair of CsA‐treated mice appeared to grow from 7 days after the treatment, even at low doses. Induced hair growth was dose‐dependent and became clearly obvious 3 weeks after the treatment. The stimulation of hair growth was not restricted to the site of topical application. The distribution of the new hair depended on the natural pattern of hair growth in the mice. However, there was no hair growth in the control mice which were given only olive oil. Histological examination revealed that there were no differences in the structures of skin and hair between the control and the CsA‐treated mice. Furthermore, the number of hair follicles did not remarkably increase after CsA treatment. The hair growth in the CsA‐treated mice stopped after cessation of the treatment and returned to the level of the control mice on day 14 after the end of the treatment. Subsequent retreatment with CsA resulted in further regrowth of the hair. However, old nude mice (9 months old) revealed no hair growth at the end of a 4‐week tre

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1991.tb03162.x

年代:1991

数据来源: WILEY

摘要:

AbstractThe cytokeratin expression of Merkel cells in fetal rat skin was studied by light‐ and electron microscopy. Employing a pre‐embedding staining method, 2 monoclonal anti‐keratin antibodies (RCK‐102, MA‐902) were shown to stain Merkel cells specifically. Neighbouring keratinocytes were unstained. The staining reaction seems to be based on the expression of 52.5 kD cy

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1991.tb03163.x

年代:1991

数据来源: WILEY

摘要:

AbstractThe clinical, histopathological, and electron microscopic features often patients with confluent and reticulated papillomatosis (CRP) of Gougerot and Carteaud were studied. Histopathologically, hyperkeratosis, decreased granular layer, irregular papillomatosis, and hypermelanosis of the basal layer were present. In an electron microscopic study, we found transitional cells between the stratum granulosum and stratum corneum that were increased in CRP. This finding supported the suggestion that this is a defect of keratinization.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1991.tb03164.x

年代:1991

数据来源: WILEY

摘要:

AbstractNucleolar organizer regions (NORs) are loops of ribosomal DNA seen in nuclei, which are demonstrable as black dots (AgNOR) in tissue sections by silver (Ag) colloid staining. The number of such AgNORs is correlated with cellular activity and is an indicator of the degree of malignancy. In this study, 76 melanocytic lesions were analyzed by AgNOR staining, and the clinical and histopathological characteristics of malignant melanoma and melanocytic nevi were considered. Although the AgNOR counts for melanocytic nevi were significantly different from those in malignant melanoma, an obvious overlap between them was detected. The number of AgNORs in melanocytic nevi per cell was usually 1 or 2. On the other hand, the number of AgNORs per malignant melanoma cell was variable. Morphologically, malignant melanoma cells often showed dispersal of AgNORs throughout the nucleus as well as multiple nucleoli containing clustered AgNORs, whereas melanocytic nevus cells tended to have a regular nucleolus with tightly clustered AgNORs. The correlation between AgNOR count and pathological staging was uncertain, but a slight correlation between AgNOR count and thickness of the primary lesion was obtained. However, the AgNOR count in malignant melanoma was not a prognostic factor for the disease. Therefore, the AgNOR method is difficult to use for differential diagnosis between benign pigmented lesions and malignant melanoma. Nonetheless, an AgNOR count of more than two per cell favors a diagnosis of malignant melanoma.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1991.tb03165.x

年代:1991

数据来源: WILEY

摘要:

AbstractThe clinical features and histopathologic findings of a 54‐year‐old Korean male who had retained the sting apparatus of a bee for four months are described. The clinical features showed ulcerative, erythematous plaques with irregular borders which resembled cutaneous neoplasms such as squamous cell carcinoma. Histopathologic findings included epidermal necrosis and marked pseudoepitheliomatous hyperplasia. In the dermis, the stick‐shaped sting apparatus of the bee was demonstrated and intense lymphohistiocytic and eosinophilic infiltrations were

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1991.tb03166.x

年代:1991

数据来源: WILEY