摘要:

ABSTRACTA total of 829 patients with squamous cell carcinoma (SCC), 942 patients with basal cell carcinoma (BCC) and 256 patients with malignant melanoma (MM) was encountered among 1,033,678 new dermatology patients in 44 of 46 university hospitals of Japan between 1971 and 1975. The incidences of skin cancers (SCC and BCC) and of MM among new dermatology patients were 0.171% and 0.025% respectively. The incidences had increased 2 to 2.5 times over those of the previous survey between 1956 and 1960.Skin cancers were most frequently seen among those over 60 years of age and on exposed sites. Burn scars, chronic radiodermatitis, traumatic scars and xeroderma pigmentosum were common preceding diseases in skin cancers, although the majority of the cancers were without apparent preceding diseases. The incidence of skin cancers on exposed sites without preceding diseases showed a statistically significant exponential correlation with the decreasing degrees of the Northern Latitude (NL) of the university locations. The incidence of MM was most common on the extremities and showed no such geographic correlation.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1984.tb01484.x

年代:1984

数据来源: WILEY

摘要:

ABSTRACTThis paper describes the electron microscopic structure of dyskeratotic cells observed in cutaneous lesions of 3 cases with acquired zinc deficiency. Double‐zoned cytoplasm consisted of amorphous cell organelles and irregular tonofilaments occasionally containing lipid droplets and melanosomes. Small nuclei were seen in many dyskeratotic cells around the blister. Various degrees of alteration were noted in cells in erythematous lesions following the disappearance of all desmosomes. The halo formation of dyskeratotic cells with double‐zoned cytoplasm indicates an alteration in keratinocytes, which may be caused by superoxide radical anions resulting from inactivity of superoxide dismutase, a zinc‐dependent e

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1984.tb01485.x

年代:1984

数据来源: WILEY

摘要:

ABSTRACTTwo melanoma cell lines (HMY‐1 and HMY‐2) derived from two patients suffering from malignant melanomas have been establishedin vitro.A study of cellular immunity was conducted, with particular attention to natural killer cell cytotoxicity mediatedin vitroin one patient with melanoma (HMY‐2) using the established cell lines. During the last stages of the clinical course of the metastasized HMY‐2 patient, both autologous and allogeneic natural killer‐like cytotoxicities of her lymphocytes weakened drastically. The natural killer‐like cytotoxicity of all autologous and allogeneic lymphocytes was enhanced after their preincubation for 3 days in the presence of BCG,

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1984.tb01486.x

年代:1984

数据来源: WILEY

摘要:

ABSTRACTThe following results were obtained after electron microscopic observation of skin lesions and outgrown epidermal cells (OEC) released from explant cultures in Hailey‐Hailey's disease (HHD) and Darier's disease (DD).It was not always evident that the dissociation between keratinocytes in HHD and DD was caused by separation at the center of the desmosome; it seemed rather to be a phenomenon due to melting apart of cytoplasmic processes.Marked development and deformation of microvilli observed in keratinocytes in lesions of both diseases as well as their disappearance were considered to be phenomena reflecting the degree of cell membrane disturbance accompanying the above disjunction.In particular cases where dissociation progressed remarkably, keratinocytes became round and were released into lacunae (bullae) developed in the epidermis.Some of the keratinocytes had organelles and tonofilaments around nuclei with a circular electron transparent zone between the nuclei and cell membrane; they were presumed to correspond to “corps ronds” observed by optical microscope.Electron microscopically, these specific round cells looked finding as if, in most cases, they had been entirely phagocytized by other keratinocytes. This finding was especially marked in DD, and was designated the “corps ronds phenomenon”.Pathological changes identical with the above were observed in OEC released from explant cultures not only in DD but also in HHD. The “corps ronds phenomenon” was confirmed to occurin vitro.The nature and pathogenesis of acantholysis and dyskeratosis in DD and HHD were inferred from t

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1984.tb01487.x

年代:1984

数据来源: WILEY

摘要:

ABSTRACTNeutrophil cell function in psoriasis was studied through polymorphonuclear cell adherence to nylon wool, chemotaxis under agarose, and phagocytosis ofCandida albicans. Differences in PMN adherence were not found between psoriatics and controls. The chemotactic response to zymosan activated serum (ZAS) did not differ between psoriatic and normal PMNs. The psoriatic serum exhibited chemoattracting properties equal to ZAS. Psoriatic serum was shown to be chemoattracting to psoriatic and normal PMNs whereas normal serum was only to psoriatic cells. Differences in recognition mechanisms between psoriatic and normal cells are proposed. Phagocytosis ofCandidaby psoriatic PMNS was earlier and more prolonged and candidacidal activity was earlier and definetely increased at given times compared to normal PMNs. A protective role of psoriatic PMNs in Munro's microabcesses against bacterial invasion is postulated.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1984.tb01488.x

年代:1984

数据来源: WILEY

摘要:

ABSTRACTAn endemic peripheral vascular disorder called “blackfoot disease” has been noticed in a limited area on the southern coast of Taiwan. Clinically, the symptoms and signs of blackfoot disease (BFD) are similar to those of Buerger's disease. The main ultrastructural change in the dermal blood vessel is degeneration of the endothelial cells. Among the inhabitants of this endemic area, a remarkable percentage of chronic arsenism is also found. It is currently believed that drinking of artesian well water containing both a fluorescent substance (FC) and arsenic is the cause of these two diseases. The purpose of this study is to examine the vascular and mutagenic effects of FC and also to discuss the roles of FC and arsenic in BFD and chronic arsenism. It was found that FC has the following effects: (1) inhibition of histamine‐induced capillary permeability (2) degeneration in the capillary endothelial cellsin vivo(3) mutagenesis, and (4) enhancement of the incorporation of3H‐thymidine in low fetal calf serum containing cultured fibroblasts. This is strong evidence that FC is the primary substance affecting the endothelial cells and has an important effect in BFD. Other effects of FC include mutagenicity and increased DNA synthesis. When these effects are combined with arsenic, chronic arsenism becomes pr

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1984.tb01489.x

年代:1984

数据来源: WILEY

摘要:

ABSTRACTIn pursuit of a model for the studies of endothelial cells in a readily available experimental animal, culture methods and thein vitrocharacteristics of endothelial cells isolated from guinea pig aorta are described. Endothelial cells were harvested by trypsinization with perfusion techniques and cultured in Eagle's minimal essential medium supplemented with 10% fetal calf serum. Their passage cultivation was possible for more than six months and they have maintained thein vitromorphological characteristics of endothelial cells.The cell doubling time of the passaged endothelial cells was 115–131 hours. The growth of guinea pig aorta endothelial cells was satisfactory in Eagle's minimal essential medium containing guinea pig serum and fetal calf serum, but not horse and calf serum. Of the various concentrations of fetal calf serum in Eagle's minimal essential medium, 3% fetal calf serum did not activate cell growth and 10% fetal calf serum induced the best growth; in 20% concentration of fetal calf serum the cell growth rate decreased as compared with the rate in 10% fetal calf serum. DNA synthesis (3H‐thymidine uptake) of the cells in 20% fetal calf serum was less than that in 10% fetal calf serum. These results have no relation to cell generation nor to the cell density. With respect to the type of media supplemented with 10% fetal calf serum, Dulbecco's modified Eagle's medium, RPMI −1640 and Eagle's minimal essential medium were satisfactory for the cultivation of guinea pig aorta endothelial cells, but Medium‐199 was unsuitable. By adding endothelial cell growth supplement (75, 150 mcg) or fibroblast growth factor (100, 200 ng) to Eagle's minimal essential medium containing 3% fetal calf serum, the cell growth was stimulated approximately three times. Fibronectin‐coated wells did not activate ce

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1984.tb01490.x

年代:1984

数据来源: WILEY

摘要:

ABSTRACTPsoriasis constituted 1.46% of all the dermatological disorders seen during the one year period of the study. Males were slightly more frequently affected than females and most of the patients were in their 3rd and 4th decade. The mean age of males (36.95) was 7 years higher than that of females (29.83). Familial occurrence was recorded in 14.39% of the cases. Itching appeared to be a prominent feature; only 15.62% quoted worsening in colder months. Extensor surfaces were the commonest sites of involvement. More than one third of the cases showed nail changes of one type or the other. Psoriatic arthropathy and pustular psoriasis were rather rare.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1984.tb01491.x

年代:1984

数据来源: WILEY

摘要:

ABSTRACTSeventy one patients having fixed drug eruptions were subjected to provocation tests. The greatest number of cases were due to acetylsalicylic acid (18). Other drugs responsible were hyposcine butylbromide (15), oxyphenbutazone (14), sulphadiazine (7), tetracycline hydrochloride (6), metamizole (5) and ibuprofen (3). Other drugs were less frequent. There was evidence of cross sensitivity between various tetracyclines and sulphonamides and also between oxyphenbutazone and phenylbutazone. One patient had two groups of lesions, each reacting to a different, chemically unrelated drug.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1984.tb01492.x

年代:1984

数据来源: WILEY

摘要:

ABSTRACTThe effect of the local heat treatment on the chemotactic activity in psoriatic scales was studied by using a modified Boyden chamber method. Hyperthermic treatments were given to psoriasis plaques and scales were taken from these plaques before and after treatment. The psoriasis improved over a two week period of these treatments, but plaques were still present and scaling at that time. Treatment with local hyperthermia significantly reduced the activity for polymorphonuclear leukocytes in psoriatic scales (p<0.05).This result suggests that the inhibition of chemotactic activity may be involved in the action mechanism of hyperthermia in psoriatic lesions.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1984.tb01493.x

年代:1984

数据来源: WILEY