摘要:

ABSTRACTIn order to clarify the effects of tissue processing on the ultrastructure and immunological properties of human papilloma virus (HPV), HPV particles were studied comparatively by negative staining and immunofluorescence (IF) technique before and after processing with various fixatives, such as formalin (F), ethanol (E), formalin and ethanol (FE), and formalin, ethanol, xylol and ethanol (FEXE). No significant ultrastructural alterations were demonstrated after processing with F, but considerable alterations were seen with E and FE. IF studies using antiserum against raw HPV revealed a remarkable decrease in specific fluorescence (SF) after processing with F or FEXE, and the disappearance of SF with E alone. However, IF studies using antiserum against formalin‐processed HPV revealed identical SF before and after processing with F or FEXE. From these findings, it is concluded that the ultrastructure of HPV may to some degree be altered by usual tissue processing, and that the reactivity of HPV to the antiserum is most seriously affected by formalin fixation of the tissue processing methods employed in preparing deparaffinized section

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1982.tb02608.x

年代:1982

数据来源: WILEY

摘要:

ABSTRACTUltrastructural investigations were performed on human sole skin after spray cryosurgery with liquid nitrogen, using a one cycle (single freeze and thaw) method and a two cycle (double freeze and thaw) method.In the one cycle method, the epidermal cells from the basal cell layer to the spinous cell layer showed characteristic changes,i.e., nuclear chromatin forming condensed masses, the appearance of remarkable perinuclear edematous regions, tonofibrils pushed out towards the periphery of the cells and narrow intercellular spaces with intact desmosomes.In the two cycle method, homogeneous, less dense areas were seen in the nuclei of epidermal cells from the basal cell layer to the spinous cell layer. Wide, less dense, edematous, perinuclear areas were seen in which organelles,i.e. mitochondria, endoplasmic reticulum, and vacuoles were located. Tonofibrils were pushed out to the periphery of the cells. Complete cryonecrosis was observed in cells from the basal layer to the spinous layer.Ultrastructural changes differed in degree between the one cycle freezing method and the two cycle freezing method after spray cryosurgery.It was concluded that the cryosurgical technique using the two cycle method was more effective in inducing complete cryonecrosis.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1982.tb02609.x

年代:1982

数据来源: WILEY

摘要:

ABSTRACTThe distribution of secretory IgA [sIgA] components (IgA, secretory component [SC]and J‐chain) was investigated in normal skin, pathological skin, and the mucous membrane immunofluorescently.In three of fifteen normal skin samples, very weak deposits of sIgA‐components were detected at the upper portion of the epidermis and SC was found weakly at the dermo‐epidermal (D‐E) junction. Normal mucous membrane from the lip, the oral cavity, and the glans penis showed the presence of sIgA components in the upper, vacuolated epithelial cells. In one of three specimens from the oral cavity, deposition of SC was found weakly at the proprial‐epithelial (P‐E) junction. IgA‐bearing cells were detected in the proprial layer. In the pathological skin showing epidermal involvement, marked visible deposits of SC were found at the D‐E junction and also around dilated vessels in the dermal papillae. In aphthous lesions of the oral cavity, SC was located at the P‐E junction. SIgA components were detectable in the stratum corneum of these skin diseases and on the surface of involved mucosae.These findings may suggest that sIgA is concerned with the surface immunological defence system of the skin and

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1982.tb02610.x

年代:1982

数据来源: WILEY

摘要:

ABSTRACTUsing transmission electron microscopy, the intraepidermal free nerve endings in the back skin of spotted black and white guinea pigs were examined. Many intraepidermal free nerve endings were present in both the black areas and the white areas. They were located in the spaces between the basal lamina of the epidermis and the cytomembrane of the basal keratinocyte or in the intercellular spaces between the basal keratinocytes, but never beyond the Malphigian layers. Most of them appeared to belong to sensory nerves and some to adrenergic nerves. They seemed to be related intimately to epidermal keratinocytes.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1982.tb02611.x

年代:1982

数据来源: WILEY

摘要:

ABSTRACTUsing pure pig skin epidermis, we investigated the biochemical nature of adenosine deaminase, Two different (large and small) forms of adenosine deaminase were separated by a gel filtration technique using Sephadex G‐150 column chromatography. The molecular weight of the large form was 300,000 to 350,000 and that of the small form was 30,000 to 40,000.The kinetics of the two enzymes were quite similar, in terms of Km values for adenosine, substrate specificity, pH optima, temperature sensitivity, isoelectric points, and inhibition by coformycin, a tight‐binding inhibitor of adenosine deamin

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1982.tb02612.x

年代:1982

数据来源: WILEY

摘要:

ABSTRACTThe distribution of poly(adenosine diphosphate‐ribose) [poly(ADP‐ribose)] synthesis in human skin was investigated by an indirect immunofluorescence technique using a specific antibody against poly(ADP‐ribose). In normal skin, the specific immunofluorescence of poly(ADP‐ribose) was observed in nuclei of not only epidermal cells but also cells in the dermis, including the hair follicle and sweat gland. Preincubation of tissue sections with NAD, a substrate for poly(ADP‐ribose) synthesis, intensified the immunofluorescence of poly(ADP‐ribose). Nuclear immunofluorescence was prominent in the lower and middle layers of the epidermis and became weaker in the upper layers in parallel with the disappearance of the nuclei, especially in tissues untreated with NAD.The immunostaining pattern of poly(ADP‐ribose) synthesis in psoriatic epidermis was similar to that of normal skin except that fluorescence was observed in nuclei of the parakeratotic horny layer.In affected cancerous skin, immunofluorescence of poly(ADP‐ribose) was weak in nuclei of cells in the inner layers of horn pearl of well differentiated squamous cell carcinoma, although fluorescence was prominent in the outer layer cells of horn pearl. In contrast, tumor cells of both malignant melanoma and basal cell epithelioma exhibited the nuclear immunofluorescence of poly(ADP‐ribose) synthesis. These results suggest that the capacity for synthesizing poly(ADP‐ribose) may be related to the differentiation

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1982.tb02613.x

年代:1982

数据来源: WILEY

摘要:

ABSTRACTStudies of the association between porphyria cutanea tarda (PCT) and primary liver cancer have produced conflicting reports. The incidence of this liver tumor was studied in 138 PCT patients (7.2%) and in 358 non‐porphyric cirrhotic patients (5.6%), and no statistically significant differences were found between them (p>0.5). Nevertheless the incidence of such association was significantly higher (chi square=10.91, p<0.001) in the PCT group (24.3%) than in the non‐porphyric cirrhotic group (6.7%) when only males with underlying liver cirrhosis were considered. These results suggest that the high incidence of liver tumor in PCT is not merely related to hepatic cirrhosis. The cause of such as association remains unclear. Primary liver cancer may reveal a prior deficiency in uroporphyrinogen‐decarboxylase, but the possibility that the enzyme abnormalities were caused by the liver carcinoma also e

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1982.tb02614.x

年代:1982

数据来源: WILEY

摘要:

ABSTRACTWe examined a set of three‐year‐old Japanese dizygotic twins with lipodystrophia centrifugalis abdominalis infantilis. One had depressed skin lesions on the left side of her neck and in her axillary area, while the other had a lesion on her right inguinal area.Although their MLC were identical, they differed from the MLC of an unrelated patient who suffered from the same disease. HLA‐BW 51 was found in all three pat

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1982.tb02615.x

年代:1982

数据来源: WILEY

摘要:

ABSTRACTA case of Reiter's disease in a twenty‐four year old male is described. He developed deformities of the big joints twelve years prior to the appearance of the skin eruptions. The clinical and radiological findings were fairly conclusiv

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1982.tb02616.x

年代:1982

数据来源: WILEY

摘要:

ABSTRACTA seventy‐five year old man has had numerous papules over his entire body for about 50 years without any systemic involvement. Histological examination revealed that many plasma cells and a few lymphoid cells had infiltrated into the dermis. The plasma cells did not show any atypia. Laboratory examinations, including Bence‐Jones' protein, serum gammaglobulin, and immunoglobulins, demonstrated no abnormal findings. We consider this case not to be a true tumor but rather a benign reactive proliferation of plasma cells in the skin, although its etiology is uncl

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1982.tb02617.x

年代:1982

数据来源: WILEY