摘要:

AbstractWe studied ultracytochemically how trichohyalin granules (TGs) were involved in the cornification of the inner root sheath (IRS) cells in the hair follicle. After storing unfixed mouse skin samples in glycerol and then low ionic strength salt solution (G‐LISS), the TGs reduced electron‐dense amorphous material (EDM) to various degrees, with the appearance of filamentous material in routine staining. As the IRS cells were differentiating, these changes in the TGs became successively prominent; Some TGs consisted of the filamentous material and residual granular EDM, showing a “filamentogranular structure”. In the cells in transition to cornified cells, or transitional cells, the EDM of the TGs disappeared and consisted of only filamentous material which was intertwined with keratin filaments (KFs). The internal substructure of the TGs induced by the G‐LISS treatment stained well with ethanolic phosphotungstic acid, which reacts with basic proteins, and the KFs associated with the TGs also stained well. In skin samples not treated with G‐LISS, the TGs of the transitional cells often exhibited fibrogranular structure after PTA staining to detect tissue basic proteins, suggesting a release of PTA stainable proteins(s) by the TGs in living transitional cells. The KFs of the cornified cells were more intensively stained and thicker (about 20 nm wide) than those of the differentiating IRS cells. These findings suggest that the TGs may contain at least two kinds of basic proteins; One is G‐LISS‐soluble, and the other is a very insoluble filamentous protein. These protein materials may be added to or incorporated into the KFs in the cornified cells with the disappearance of the TGs, since the KFs are markedly thick wi

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1989.tb01228.x

年代:1989

数据来源: WILEY

摘要:

AbstractA monoclonal antibody, ERD5, which recognizes a 29Kd phosphoprotein associated with human estrogen receptor of myometrium was used to study the expression of this protein in normal skin and in cultured human keratinocytes. By indirect immunofluorescence, bothin vivoandin vitrokeratinocytes showed a variable cytoplasmic staining which increased with cell differentiation. SDS gel electrophoresis of soluble extracts of cultured keratinocytes and normal epidermis showed that P29 was a minor protein. Immunoblot analysis demonstrated that ERD5 strongly reacted only with a 29Kd polypeptide band without any cross‐reactivity.These data suggest that keratinocytes might be estrogen sensitive like other cells in which P29 has already been located. The exact role of this protein in the keratinocyte differentiation process and its relationship with estrogen receptors remain to be elucidate

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1989.tb01229.x

年代:1989

数据来源: WILEY

摘要:

AbstractThe purpose of the present study was to compare the degrees of nonenzymatically glycosylated proteins in the skin (stratum corneum), the nail, the hair, and hemoglobin obtained simultaneously from the same subject and to evaluate the most useful sample for management of diabetic complications. Fifty‐one diabetic patients and 20 control patients were examined, utilizing furosine determination. Furosine value of the skin in diabetics was 2.14 ± 1.70%, whereas that in controls was 1.65 ± 0.47%. Furosine value of the nail in diabetics was 6.67 ± 3.30%, whereas that in controls was 4.16 ± 1.62%. Furosine value of the hair in diabetics was 1.30 ± 1.11%, whereas that in controls was 1.29 ± 1.71%. Close correlations were detected between HbA1(glycosylated hemoglobin) and furosine of the nail (r=0.58, p<0.001), HbA1and furosine of the skin (r=0.48, p<0.001), and HbA1and furosine of the hair (r=0.43, p<0.01); however, poor correlations were found between furosine of the hair and the skin (r=0.35, p<0.05) and furosine of the nail and the hair (r=0.33, p<0.05). Furosine of the nail was significantly correlated with the FBS (fasting blood sugar) of the same time, previous 6, and previous 12 months. Furosine value of the nail, we believe, is the most useful indicator for evaluating long term control of diabetics and may provide useful information for management of diabetic compli

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1989.tb01230.x

年代:1989

数据来源: WILEY

摘要:

AbstractInterleukin 1 (IL‐1) and Prostaglandin E2(PGE2) are mainly produced by macrophage/monocytes. In this experiment, we investigated the immunological role of macrophage/monocytes in the lymph nodes (LNs) by measuring IL‐1 and PGE2productions of regional LN cells from dinitrofluorobenzene (DNFB)‐sensitized and ‐tolerant mice. LN cells from sensitized mice produced IL‐1 in remarkable amounts afterin vitroantigen stimulation. On the other hand, the LN cells from tolerant mice failed to produce IL‐1 or PGE2. These data indicate that, by modifying cytokine production, macrophage/monocytes in the regional LNs play a key role in the pathomechanism of contact hyper

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1989.tb01231.x

年代:1989

数据来源: WILEY

摘要:

AbstractWe studied the porphyrin metabolism of a 7‐year‐old Japanese boy with erythropoietic protoporphyria (EPP) and his family members. Leukocyte ferrochelatase activity was markedly decreased in this patient, being approximately 12% of the mean value of normal controls (4 aged‐matched healthy boys). In contrast, leukocyte δ‐aminolevulinic acid (ALA) synthase activity was normal. The free protoporphyrin content of erythrocytes was greatly increased (4.3 mg/100 ml RBC), while erythrocyte ALA dehydratase and porphobilinogen (PBG) deaminase activities were 1.7‐ and 2.2‐fold of respective control values. A survey of his family revealed that 12 of 19 members probably had manifest EPP or were EPP carriers. These results suggest that, in EPP, there might be an inherited impairement of ferrochelatase activity which gives rise to an elevation of erythroblast ALA dehydratase and PBG deaminase activities to compensate for a resultant decrease in hem

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1989.tb01232.x

年代:1989

数据来源: WILEY

摘要:

AbstractA 51‐year‐old Japanese male developed morphea profunda over a plaque of pigmented sweating skin on the left chest extending deeply into the lung. Eight years previously, he noted a brown pigmented spot on the left axilla which started to enlarge over the left chest in a band‐like manner. Later, the involved skin hardened with profuse sweating. Chest X‐ray examination and computer tomography revealed a thickening of the left chest wall and a nodular mass lesion in the left lower lung field. Biopsy specimens from lung, chest wall, and overlying skin revealed a granulomatous tissue in the parenchyma and a marked fibrosis extending from the deep dermis to the pleura. There were no findings suggestive of malignancy or chronic infectious disease. We assume that the process of morphea in this patient extended deeply from the skin to t

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1989.tb01233.x

年代:1989

数据来源: WILEY

摘要:

AbstractStudy of the histopathologic pattern of 28 cases of superficial granulomatous pyoderma demonstrated an ulcerative, verrucous pyoderma of the superficial dermis. Focal abscesses in the subepidermis or dermis were juxtaposed with granuloma formation and plasma cell inflammation. Hemorrhage and granulation tissue were present. The clinical lesions are indolent, and this feature is confirmed by chronic inflammatory elements, including sinus tract formation and focal fibrosis. Superficial granulomatous pyoderma must be added to the histologic differential diagnosis of the vegetative and ulcerative granulomas: blastomycosis, tuberculosis verrucosa cutis, and bromoderma.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1989.tb01234.x

年代:1989

数据来源: WILEY

摘要:

AbstractThe duct‐like structures present in 7 cases of benign and malignant eccrine poroma were examined by immunohistochemical staining for carcinoembryonic antigen (CFA), involucrin, and S‐100 protein. The demonstration of CEA and involucrin was helpful in the recognition of these structures. The overt immunopositivity precedes morphological evidence for duct formation. On the basis of the CEA immunostaining, the duct‐like structures were divided into 4 types: 1) mature acrosyringeal structure, 2) cystic luminal structure lined by elongated cells, 3) immature acrosyringeal structure, and 4) vacuole‐ or dot‐like potential lumen in a single cell. Involucrine was observed in the lining cells of 1) and 2). None of the 4 types showed positive reactivity for S‐100 protein, suggesting the irrelevance of these structures to the secretory element of sweat gland. The polymorphism of the ductal formation tended to be more remarkably observed in malignant eccrine poromas than in the cases of benign eccrine poroma and poroepithel

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1989.tb01235.x

年代:1989

数据来源: WILEY

摘要:

AbstractWe report here an 11‐year‐old Japanese girl with an ulcer‐forming type of mosquito hypersensitivity. Since the lesions might have arisen from an Arthus type of hypersensitivity, we used a phorbol myristate acetate (PMA)‐triggered chentiluminescence method to investigate the function of her peripheral monocytes, which should play an important role in any Arthus reaction in association with polymorphonuclear leucocytes (PMNs). The chemiluminescence of the patient's monocytes was significantly reduced from that of the normal control. To our best knowledge, no such functional abnormality of the monocytes has been seen previously in patients with an ulcer‐forming type of mosquito hyperse

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1989.tb01236.x

年代:1989

数据来源: WILEY

摘要:

AbstractA case of a female with a large cystic tumor in her infrascapular region is reported. The tumor was 8 cm × 7 cm in size. Histologically, it was a pseudocyst with calcification. The patient showed no abnormal renal functions other than proteinuria. Because she had suffered from overlap syndromes of systemic lupus erythematosus, progressive systemic sclerosis, and suspected dermatomyositis, we diagnosed this as a unique case of dystrophic calcinosis. There have been several reports of cases with connective tissue diseases showing subcutaneous calcification, but to our knowledge no such giant calcifying pseudocyst has ever been reported

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1989.tb01237.x

年代:1989

数据来源: WILEY