摘要:

ABSTRACTKeratin subunits of 64K, 53K, and 43K M.W. from human plantar stratum corneum fibrous proteins were isolated by SDS polyacrylamide gel electrophoresis (PAGE), and the antiserum against each keratin was prepared from rabbits. During indirect immunofluorescence studies of the normal skin, the whole epidermis stained positively with anti‐64K keratin antiserum (64K antiserum), but the positive staining of the basal layer diminished with the dilution of the antiserum. A similar staining pattern was observed with 53K antiserum, although the diminished staining of the basal layer was much less obvious than that with 64K antiserum. In contrast, 43K keratin was distributed throughout the whole epidermis including the basal layer, and no diminished staining with dilution of the antiserum was observed. These results suggest that an immunofluorescence technique using anti‐large M.W. keratin antisera, especially 64K antiserum with a certain degree of dilution, could be used to study the differentiated cells of the epidermis.DEAE‐cellulose column chromatography of keratins revealed that 64K keratin, along with some of the smaller M.W. keratins, was eluted at a low concentration of KCl and could be separated from most of the smaller M.W. keratins, which were eluted at higher concentrations of KCl. In the first process of stratum corneum fibrous protein extraction, 64K protein was observed to be extracted with neutral Tris‐HCl buffer. This protein was found to be immuno‐reactive with the antiserum to 64K keratin which had been extracted with Tris‐HCl buffer containing urea and mercaptoethanol. It is suggested that the biochemical properties of large M.W. keratin are somewhat different from those of smaller M.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01142.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTTo investigate the process of keratin formation, changes in polypeptide composition of epidermal keratin and of prekeratin were examined using serial slices of human skin. In most of the skin surface, prekeratin was composed of 70K, 58K, 56K, 49.5K and occasionally 55K polypeptides, and an additional 63K polypeptide was present in the skin of the palm and sole. The decrease in molecular weight of these prekeratins to that of keratins was observed in the region of the granular and transient cell layers, especially in that of palm and sole. This was partially confirmed by preliminary digestion experimentsin vitro. Our present studies suggest that limited proteolysis of higher molecular weight prekeratin(s) is a step in keratin formation of the stratum corneum.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01143.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTUsing anin vitroexplant culture system of pig skin, the effects of calcium and calcium‐ionophore on the outgrowing epidermis were studied. The optimum concentration of calcium on the rates of epidermal outgrowth was 1.0 mM and that of mitosis was around 1.2 mM. Ionophore A23187 (Ionophore) significantly stimulated both the rates of epidermal outgrowth and the mitosis of the outgrowing epidermis. This stimulation was partially blocked by the addition of hydrocortisone.The cyclo‐oxygenation products, prostaglandins, failed to stimulate the rate of epidermal outgrowth. It appeared that the stimulatory effect of Ionophore on the outgrowing epidermis was due either to the activation of phospholipase A2or to the activation of the lipoxygenase path

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01144.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTIt has been reported that hydrocortisone had a protective effect on beta‐adrenergic adenylate cyclase system of the pig epidermis duringin vitrolong term incubation. Using the same experimental system, the effects of another glucocorticoid, betamethasone 17 valerate were investigated. In short term experiments (5 min incubation time), betamethasone had no effect on the cyclic AMP accumulation of the epidermis. When skin slices were incubated with this compound for 48 hours, the response of the skin to epinephrine was preserved and showed a greater accumulation of cyclic AMP in betamethasone‐treated skin than in the control. This effect was observed at concentrations greater than 0.001μM of betamethasone, with the maximal accumulation of cyclic AMP at 0.1μM. Neither histamine responsiveness nor cyclic AMP phosphodiesterase activities (low and high Km enzymes) were altered by the betamethasone treatment. Thus, betamethasone showed a protective effect against the beta‐adrenergic adenylate cyclase system similar to that of hydrocortisone in much lower concentrations.These effects of glucocorticoids would be the essential pharmacological action of this type of hormone on epidermis, and glucocorticoids might reveal their biological activity through the cyclic AMP system of t

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01145.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTThe extracellular keratinase fromM. caniswas purified using ion exchange chromatography and gel filtration. The purified enzyme showed a single protein band on sodium dodecyl sulfate‐polyacrilamide gel electrophoresis. A molecular weight of approximately 45,000 was determined by SDS‐electrophoresis.Anti‐body directed against the purified enzyme fromM. caniswas obtained from New Zealand White rabbits, and the immunological identity of the enzyme fromM. canis, as well as those fromM. gypseum, T. mentagrophytesandT. rubrum, were demonstrated by immuno‐double‐diffusion ex

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01146.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTTo gain further insight into the mechanism of immune deposition in the skin, antigens and antibodies were administered intravenously at different times into normal guinea pigs, and the presence of immune complexes in frozen sections of skin was determined immunohistochemically, using light microscopy. Cutaneous immune deposits were found after primary injection of anti‐horseradish peroxidase antibody (IgG) followed by secondary administration of horseradish peroxidase, while no immune complexes were detected after the same injections in the reverse order. This disparity may possibly be explained by the length of time the antigen or antibody remains in the skin. Using a primary injection of bovine serum albumin (BSA), followed by secondary injection of anti‐BSA antibody, immune deposits were demonstrated in the skin. In these cases, if the initially injected antigen or antibody remained for a long time at the site of exudation, they would react with and form immune complexes with the subsequently injected antibody or antigen at the same s

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01147.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTDistribution of immunoglobulins in normal human epidermis was revealed at both the light and electron microscopic levels using peroxidase labeled antibodies. Biopsy specimens were obtained from various body locations of 40 healthy persons from 2 to 82 years old under local anesthesia. There were three types of IgG staining patterns in normal human epidermis. Intraepidermal IgG was found in about 80% of the skin samples. IgG was localized in the cytosol of squamous cells by electron microscopy. There were no morphological differences between the IgG positive and IgG negative cells. There seemed to be a correlation between IgG staining and the IgG serum level. The results of the present study suggest that with highly sensitive immunohistochemical techniques, IgG can be detected not only in diseased skin but also in normal skin.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01148.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTA 60‐year‐old Japanese man with a five year history of bullous lesions associated with scar and milia is described. This case seemed to have an unusual type of bullous pemphigoid clinically, histologically and immunofluorescently, but was found to have epidermolysis bullosa acquisita (EBA) based on electron microscopic and immunoelectron microscopic findings, in which amorphous substance and immunological reaction products were detected mainly below the subbasal lamina‐anchoring fibril

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01149.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTThis paper proposes a new skin disease entity—pityriasis pigmentosa. Characteristic lesions of this disease are well defined, oval or round, pigmented patches covered with fine, adherent, wrinkled scales. The size of the lesions varies from 0.5–3 cm in diameter. Some lesions gyrate or coalesce into larger patches. The lesions are typically located on the waist and the extremities and are particularly common on the volar aspect of the upper arms and the posterior aspect of the upper thighs. Using Wood's light examination, a bright golden fluorescence is inevitably seen. Rapid identification can be made by use of the KOH/Parker Ink preparation. This reveals characteristic branched, segmented hyphae and clusters of budding, oval‐shaped yeast cells.Pityrosporum ovaleproduces hyphae when cultured in glycine‐ammonium phosphate buffered media.Pityrosporum orbicularedoes not produce hyphae by this method. These clinical and laboratory findings distinguish a clinical entity differing from Tinea (Pityriasis) versicolor which is caused byPityrosporum orb

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01150.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTFour cases of Dowling Degos disease (DDD) in one family and five cases of Haber's syndrome (HS) in two families were compared. DDD and HS share many symptoms and findings in common; histopathological features of downgrowths of epithelial cells, papules of either brown or natural skin color, and various kinds of skin pigmentation. Some had pitted scars, cysts, pruritus, telangiectasia, red cheeks, xerosis, and photosensitivity. Another feature common to both conditions is the progressive course of the disease; patients and their children should be followed over their lifetimes. One case of DDD had basal cell epithelioma within the area of reticular pigmentation, but an 83‐year‐old patient with DDD indicated that general health is not involved in DDD.One differentiating point for HS is that black warts in HS do not have the histologic features of DDD; they show only seborrheic keratosis. In HS, microscopic examinations should be made of as many varied lesions as possible.HS appears to be a subtype of DDD, characterized by red facies, seborrheic keratosis, and earlier onset than DDD. Both DDD and HS appear to be conditions with a different reactivity of the melanocyte‐keratinocyte system to external stimulation, rather than a disease of nevoid o

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01151.x

年代:1983

数据来源: WILEY