ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1982.tb02633.x

年代:1982

数据来源: WILEY

摘要:

AbstractThe effectiveness of DTIC (5‐(3.3‐dimethyl‐1‐triazeno) imidazole‐4‐carboxamide) against a human malignant melanoma cell line (SEKI, II) was investigated with the additional use of fluorescent light and long wave ultraviolet irradiation. The cell cycle phase distribution of cultured melanoma cells exposed to DTIC (10μg/ml, 100μg/ml) was analyzed by means of flow‐cytometry (ICP type 11). The cytostatic effect of DTIC was observed at a concentration higher than 10μg/ml in standard culture. Remarkable enhancement of the effect of DTIC was observed in both fluorescent light and ultraviolet irradiation. The most prominent change in cell cycle phase occurred in the group treated with DTIC 100μg/ml with fluorescent light. S phase prolongation was shown in early stages (6, 12 hours), and then G2M phase accumulation was observed in late stages (48 hours). In ultraviolet irradiation groups, enhancement of the cytocidal effect of DTIC was observed 12 hours after irradiation. The death of all of the cells was observed in the DTIC 10μg/ml + UVA 3 joules/cm2and the DTIC 100μg/ml + UVA 1 joule/cm2groups. DTIC with UVA irradiation may be used for the treatment of cutaneo

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1982.tb02634.x

年代:1982

数据来源: WILEY

摘要:

AbstractThe cell cycle phase distribution of a human malignant melanoma cell line (SEKI, II) exposed to various chemotherapeutic agents was investigated by flow‐cytometry. The accumulation effect on the radiosensitive G2and M phases was nearly 100% in the vincristine 0.1μg/ml group, 75.2% in the ACNU 5μg/ml group, and 53.1 % in the pepleomycin 1μg/ml group, respectively. The groups treated with adriamycin (1μg/ml and 10μg/ml) or prednisolone (10μg/ml) were not significantly different from the control group. It is suggested that vincristine, ACNU and pepleomycin may be useful for synchronization radiation

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1982.tb02635.x

年代:1982

数据来源: WILEY

摘要:

AbstractThe growth inhibition and cell cycle phase distribution of a human malignant melanoma cell line (SEKI, II) treated with 8‐methoxypsoralen (8MOP) and UVA irradiation (PUVA) were investigatedin vitro. A growth inhibition effect was observed in 8 methoxypsoralen (8MOP) 0.1μg/ml + UVA 1 J/cm2group, and a strong cytocidal effect was noted in the 8MOP 1μg/ml + UVA 3 J/cm2groups. Continued accumulation was observed at 24 to 72 hours in the following phases: G2M phase in the 8MOP 0.1μg/ml + UVA 1 J/cm2group; middle S phase in the 8MOP 0.1μg/ml + UVA 3 J/cm2group; and early S phase in the 8MOP 1μg/ml + UVA 1 J/cm2group. It appeared that 8MOP with UVA irradiation acts mainly on the S phase to block the cell cycle progression between the S and G2M phases. The variation of cell cycle phase distribution under the effect of 8MOP with UVA irradiation was similar to that observed with the alkylating agent, ACNU, suggesting a possible similarity of the mechanism of action between them. The reaction in the dark was vital for the onset of action of 8MOP with UVA irradiation; as is the case for DTIC with UVA irradiatio

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1982.tb02636.x

年代:1982

数据来源: WILEY

摘要:

AbstractClinicopathological and statistical studies were made on one hundred and fifty‐six Japanese cases of basal cell epithelioma. A new formula was presented for the calculation of the growth rate of BCE, as follows,Size of tumor (major axis × minor axis) (mm2) = 1.7363 × T2+ 133.1192T = Years which had elapsed from the presumed time of onset to the time of the first examinationThe yearly growth in diameter calculated from the above formula is less than 1.0 mm within ten years after onset, and 1.0 mm to 1.3 mm thereafter. The time needed for BCE to double its size averaged 8.8 years in the present study.In addition to the slow rate of progression, BCE in the Japanese was characterized by its pigmentation; 84% were either partially or totally pigmented, giving the BCE the so‐called black and pearly appea

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1982.tb02637.x

年代:1982

数据来源: WILEY

摘要:

AbstractThree cases of an atrophic type of senile keratosis were examined by electron microscopy. All three cases showed similar changes, although some variability from case to case was observed. Lesional epidermis showed remarkable widening of the intercellular spaces with a decrease in the number of desmosomes and detachment of desmosomal connections. Formation of intracytoplasmic vacuoles was also prominent. These changes were localized mainly in the lower part of the epidermis, while the upper epidermis was relatively intact. Discontinuity and multiplication of the epidermal basal lamina were sometimes observed. From these findings, it is concluded that this atrophic type of senile keratosis is characterized by ‘déhiscence cellulaire' with minimal dyskeratosis, although it can properly be regarded as an epidermal precancerous conditi

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1982.tb02638.x

年代:1982

数据来源: WILEY

摘要:

AbstractHistopathological and ultrastructural studies were carried out on a case of a 4‐year‐old boy with type II Ehlers‐Danlos Syndrome. The patient showed velvety skin, hyperelasticity of the skin, and mild hypermobility of the joints. Histopathologically, numerous very fine collagen fibers were observed in the dermis, and no recognisable group substance was found. Ultrastructurally, abnormal collagen fibrils with irregular profiles were interspersed in the reticular dermis. However, their periodic band pattern was that of normal skin collagen. It is supposed that these abnormalities of fibrils are due to abnormalities of collagen fibrilloge

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1982.tb02639.x

年代:1982

数据来源: WILEY

摘要:

AbstractSixty‐eight xeroderma pigmentosum (XP) patients, including 41 of Group A and 27 of XP‐variant, were examined for skin malignancies such as squamous cell carcinoma (SCC), basal cell epithelioma (BCE), and malignant melanoma (MM).Approximately half of the patients developed skin malignancies on sunlight‐exposed areas. The most frequent skin malignancy in both groups was BCE. In this survey, two cases with MM were found for the first time in Japan, one in Group A and one in XP‐variant. The most important difference between Group A and XP‐variant was in the age of onset of skin malignancies. The average age at the first excision of skin malignancies was 9 in Group A, while that of XP‐variant was 32. In Group A, the youngest case was 3 years old, and most of the cases with skin malignancies were younger than 15. In XP‐variant, on the contrary, the youngest case was 15 and most cases were older than 20. XP‐variant patients were apt to have multiple lesions of BCE. The ratio of BCE to SCC was about 3:1 in Group A, and 8:1 in XP‐variant. The strong tendency toward malignancy was seen only on the sk

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1982.tb02640.x

年代:1982

数据来源: WILEY

摘要:

AbstractBlood and plasma viscosity in patients with vascular disease was measured using a rotational cone‐plate viscometer. Increased blood viscosity at moderate (76.8 sec–1) and high (384.0 sec–1) shear rates was found in patients with Bürger's disease. Reduced blood viscosity was noted in patients with livedo vasculitis.Plasma viscosity was significantly increased at low (19.2 sec–1), moderate, and high shear rates in patients with leg ulcer, erythema nodosum, and Bürger's disease, in which blood vessels in the lower dermis and subcutaneous tissue were suspected to be affected. Plasma viscosity at low and moderate shear rates was increased in patients with livedo vasculitis and increased plasma viscosity at low shear rate was noted in patients with superficial vasculitis (anaphylactoid purpura and chronic pigmented purpura).Changes in plasma viscosity correlated well with amounts ofγ‐globulin, but not with amounts o

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1982.tb02641.x

年代:1982

数据来源: WILEY

摘要:

ABSTRACTA case of painful eccrine angiomatous nevus of the sole in a five‐year‐old boy is presen

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1982.tb02642.x

年代:1982

数据来源: WILEY