ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1985.tb01563.x

年代:1985

数据来源: WILEY

摘要:

ABSTRACTTo confirm whether cultured human keratinocytes derived from a normal subject and a xeroderma pigmentosum (XP) patient (genetic complementation group A) show differences in their DNA repair systems, as has been shown in normal and XP‐fibroblasts, unscheduled DNA syntheses (UDS) were compared between the primary‐cultured keratinocytes and fibroblasts after UV irradiation. The levels of UDS were low in XP‐fibroblasts but high in normal‐fibroblasts. However, only a slight difference in UDS levels between normal‐keratinocytes and XP‐keratinocytes was found. The levels of UDS were low in both normal‐ and XP‐keratinocytes as compared with nor

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1985.tb01564.x

年代:1985

数据来源: WILEY

摘要:

ABSTRACTFecal flora in two female patients with acrodermatitis enteropathica (AE) were investigated in connection with the efficacies of zinc sulfate and Entero‐Vioform (EV). The following results were obtained.Drastic deviations from normal were found in the bacterial distributions in the feces of patients before treatment. In particular,Eubacterium, one of the commonest anaerobic bacteria in normal human feces, was not detected.This characteristic finding was not altered by administration of zinc sulfate, although all clinical symptoms in the patients clearly improved.The administration of EV, however, did return the numbers of this organism to almost normal levels, with clear improvement of clinical symptoms similar to that obtained with zinc sulfate.These results suggest that intestinal flora may play an important role in the absorption of zinc from ordinary diet

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1985.tb01565.x

年代:1985

数据来源: WILEY

摘要:

ABSTRACTChemotaxis under agarose of psoriatic mixed mononuclear cells (MNs) and pure monocytes against Zymosan treated sera (ZAS), fresh psoriatic serum, and fresh normal serum was studied and compared with that of normal cells. Purification of monocytes was achieved on microexudate coated BHK flasks. Psoriatic MNs were found to be more chemotactic against psoriatic serum (P.S.) than normal MNs against normal serum (N.S.). Psoriatic cells were chemotactically more active than normal ones against psoriatic and normal serum but not against ZAS. Mixed mononuclear cells showed definitely increased chemotaxis over that of pure monocytes. When culture supernatants with lymphocyte derived factor (LDCF) activity were mixed with normal pure monocytes, the chemotactic activity of the monocytes recovered. It is, therefore, proposed that the LDCF fromin vivostimulated psoriatic lymphocytes by antigenic or mitogenic substances of psoriatic plaque might be an essential factor for the increased chemotaxis of mixed psoriatic MNs compared to pure monocytes. This hypothesis also explains the increased chemoattracting capacity of psoriatic serum for psoriatic and normal MNs as due to thein vivorelease of lymphokine.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1985.tb01566.x

年代:1985

数据来源: WILEY

摘要:

ABSTRACTLangerhans' cells were studied in the epidermis of two patients with Granuloma Annulare (GA) of the generalized type and compared with those in two normal controls. The percentage of Langerhans' cells in the GA epidermis population was 3.4% and in the control, 1.8%. The density of Langerhans' cells in different adjacent sites of the same epidermis was not uniform, being in the range of 0.9–5% in GA and 0.6–4% in controls. In the GA epidermis, the Langerhans' cell section area was increased by about 40% and the number of Langerhans' cell granules found per cell section by about 41%. The findings may indicate that, in granuloma annulare epidermis, the Langerhans' cells are in a more active st

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1985.tb01567.x

年代:1985

数据来源: WILEY

摘要:

ABSTRACTThe stability of all‐trans‐retinoic acid, 13‐cis‐retinoic acid and Ro10‐9359 (etretinate) in culture was analyzed by measuring the amount of each retinoid in culture medium with HPLC. In cultures of human keratinocytes and B16 mouse melanoma cells (K440B), all‐trans‐retinoic acid was fairly stable, but it disappeared rapidly in cultures of HeLa cells. In general, 13‐cis‐retinoic acid was more unstable. When all‐trans‐ or 13‐cis‐retinoic acid was used, the 13‐cis or all‐trans forms also appeared in the culture medium as a result of cis‐trans isomerization. Human epidermal keratinocytes were able to de‐esterify Ro10‐9359, and Ro10‐1670 was also detected in the culture medium. K440B cells had a strong capacity for de‐esterifying Ro10‐9359. When 10 nmole/ml Ro10‐9359 was added to the culture, about 1 nmole/ml of Ro10‐1670 was maintained from 24 to 96 h later. In the HeLa cells culture, no Ro10‐1670 was detected after the addition of 1 nmole/ml Ro10‐9359. These results indicate that, in the tissue culture system, the pharmacokinetics of each retinoid depended upon its conc

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1985.tb01568.x

年代:1985

数据来源: WILEY

摘要:

ABSTRACTFifty untreated and uncomplicated leprosy patients comprising three with TT, twenty‐six with BT, ten with BB, four with BL and seven with LL formed the subject material for the study. They were subjected to detailed morphological, bacteriological, histopathological and immunological investigations. Four investigators undertook the study and each one independently investigated one parameter according to a pre‐conceived standard set. The data were pooled and analysed after breaking the code. A good correlation amongst all four parameters was seen only in 44%. A striking variation in three other parameters was found in 56%, primarily in the borderline groups suggesting subclinical bacteriological, histopathological and immunological instability, either in the form of upgrading or downgrading. Similarly, the separation of five indeterminate cases from those of BT group was intrigu

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1985.tb01569.x

年代:1985

数据来源: WILEY

摘要:

ABSTRACTTen psoriatic patients were treated with topically‐applied 3‐carbethoxypsoralen (3CPs) plus UVA‐light (3CPs PUVA). Of the ten patients, five underwent this treatment more than 20 times. Psoriasis was resolved in only two of the five cases, but the effectiveness of 3CPs PUVA was not confirmed, because control sites without treatment showed similar degrees of resolution. In the other three patients, no beneficial effects were observed. One patient dropped out. The remaining four patients discontinued treatment because of severe dermatitis after five to 19 exposures to 3CPs PUVA. Three of the four were proven to be allergic to 3CPs (one, contact and photocontact, and two, photocontact allergy). Surprisingly, the psoriatic lesions in these four patients resolved spontaneously as their dermatitis faded away. UVA induced tight binding of 3CPs with human serum albuminin vitro, indicating that 3CPs can be a potent hapten for photoal

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1985.tb01570.x

年代:1985

数据来源: WILEY

摘要:

ABSTRACTCongenital onychodysplasia of the index fingers, a condition characterized by hypoplasias and dysplasias of the nails peculiarly restricted to the index fingers, is assumed to be related to a peculiar grip of the thumbs leading to ischemia of the index fingers while the baby isin utero. This is based on our observation of a case two days after birth. Major characteristics of this condition, including index finger involvement and radial‐prone involvement of the nails, can be well explained by this hypothesis, as can exceptional cases of middle finger involvement. A case of acquired polyonychia is presented which also appears to favor the ischemia theory for this conditio

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1985.tb01571.x

年代:1985

数据来源: WILEY

摘要:

ABSTRACTWe report a rare case presenting a very early, non‐invasive stage of acral lentiginous melanoma (ALM). A 67‐year‐old male patient came to our clinic 4 days after he noticed the presence of a pigmented lesion on the sole of his right heel.The lesion exhibited an irregular, asymmetrical outline and haphazard combination of colors ranging from tan to brown black; it measured 3.2 times 2.1 cm in its greatest dimensions. It was not elevated. It was surgically removed.Histopathologically, serial sections of the lesion revealed an increase in large atypical melanocytes. These abnormal cells with irregular in shape and relatively hyperchromatic nuclei, were mainly located in the basal layer, resulting in the proliferation of lentiginous type. These findings are clinically and histopathologically identical to those found in ALMin

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1985.tb01572.x

年代:1985

数据来源: WILEY