摘要:

AbstractTo investigate the mechanisms responsible for mosquito bite reaction, 120 subjects were experimentally exposed toAedes albopictusbite and two types of immunological tests were performed. Specific IgE antibodies to salivary gland extract (SGE) antigens of femaleAedes albopictuswere measured by enzyme‐linked immunosorbent assay (ELISA) in all subjects. Lymphocyte transformation tests (LTTs) to the same antigens were measured in 51 of the 120 subjects. The relationships between the results of ELISA and LTTs with bite reactions were statistically analysed. The amount of specific IgE antibodies correlated well with immediate bite reactions (r=0.55) (p<0.01). Stimulation indices (SIs) to SGE antigens correlated with delayed bite reactions (r=0.54) (p<0.01). The results suggest that type I hypersensitivity plays a role in the immediate reaction to mosquito bites and a cellular immune mechanism is responsible for the pathogenesis of delayed bite reaction

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1989.tb01277.x

年代:1989

数据来源: WILEY

摘要:

AbstractWe determined the chemotactic responsiveness of peripheral eosinophilic granulocytes (eosinophils) isolated from patients with inflammatory dermatoses and healthy volunteers. Ten patients with atopic dermatitis, five patients with drug reactions, ten patients with psoriasis, and fourteen healthy volunteers were studied. Well characterized chemotaxins, the complement split product C5a, leukotriene B4(LTB4), platelet activating factor (PAF), and N‐formyl‐methionylleucyl‐phenylalanine (FMLP), were used as chemoattractants. Eosinophils from healthy volunteers showed strong migratory responses towards C5a and PAF but responded poorly to LTB4and FMLP. When patients were grouped by disease severity, eosinophil chemotactic responses to PAF were significantly enhanced in severely affected patients (p<0.05); this was not true with C5a, LTB4or FMLP. This enhanced eosinophil chemotaxis to PAF was not related to a specific disease. No correlation between eosinophil chemotactic activity and peripheral blood eosinophil count was observed. The increased responsiveness of circulating eosinophils towards PAF may be related to altered receptor expression during cutaneous inflamm

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1989.tb01278.x

年代:1989

数据来源: WILEY

摘要:

AbstractOn the fourth day after a single painting of 2,4,6‐trinitro‐chlorobenzene on the abdominal skin, inguinal lymph nodes were removed, and a single‐cell suspension was prepared. The cells were analyzed flow cytometrically, using monoclonal anti‐protein kinase C antibody. It was found that the number of lymph node cells in which protein kinase C was detected on the cell surface was significantly increased over that in non‐treated mice (p<0.01). On the basis of our results and discussions in the literature, it is thought that protein kinase C is related to the initiation of the contact hypersensitivity

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1989.tb01279.x

年代:1989

数据来源: WILEY

摘要:

AbstractWe established a dermatofibrosarcoma protuberans (DFSP) cell line derived from a DFSP tumor from a 69‐year‐old female. The population doubling time of the cells was about 45 hours. The cells were mostly round, although a few triangular or spindle‐shaped cells were also found. They could not be transplanted into nude mice.These cells had some histiocyte characteristics which are not observed in fibroblasts: 1) Electron microscopically, there were more lysosomes and phagolysosomes than in fibroblasts. Dense bodies and myelin figures derived from lysosomes were also observed. 2) Staining with acid phosphatase (ACPase), esterase, and α‐1‐antitrypsin was positive. 3) The cells had C3 receptors but no Fc receptors. However, there were two differences between DFSP cells in the established line and those in the early stages: in the former, the capacity for immunophagocytosis (Fc receptors and phagocytosis ofCandida albicans) and collagen synthesis were almost entir

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1989.tb01280.x

年代:1989

数据来源: WILEY

摘要:

AbstractEighty‐six cases of erythema multiforme (EM) were studied based on the distribution of skin lesions. Twenty‐nine patients had distinct facial skin lesions as well as lesions on the extremities (Group A) and 57 patients had skin lesions only on the extremities (Group B). Patients in Group A were younger than those in Group B; the average ages being 7.5 and 25.2 years, respectively. In Group A, the disease occurred mainly in winter, while, in Group B, it began most frequently in summer. Preceding ulcerated pernio was seen in 11 patients of Group A, but only one of Group B. Histopathologically, both groups are compatible with a diagnosis of EM, although the seasonal occurrence of facial involvement in Group A may suggest a specific etiological relationship to c

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1989.tb01281.x

年代:1989

数据来源: WILEY

摘要:

AbstractThe authors studied the bioavailability of minocycline in sebum and serum. Blood and sebum samples were collected weekly for 6 weeks from ten healthy volunteers taking 200 mg of minocycline every day for 4 weeks. Sebum was collected by direct extraction with petroleum ether from the forehead. After evaporation, sebum was weighed on a scale accurate to 10 μg. Determination of minocycline in serum and sebum was performed using a high performance liquid chromatography technique (HPLC), with a better detection limit at 352 nm than at 400 nm (20 ng/ml and 0.324 μg/ml respectively). Our results contrast with other studies since no minocycline was detected in the sebum samples of treated subjects and microbiological assays of minocycline in sebum were also negative. In our opinion, the current hypothesis claiming that the effectiveness of minocycline in the treatment of acne vulgaris is based on sebaceous secretion should be reconsidere

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1989.tb01282.x

年代:1989

数据来源: WILEY

摘要:

AbstractA patient with SLE which was complicated by a characteristic cutaneous mucinosis is reported. The skin lesions were composed of intradermal soft nodules, purple‐red plaques, and alopecia of the scalp. Acid mucopolysaccharides accumulated in each lesion. PUVA provoked an infiltrated skin lesion in which acid mucopolysacchraides were deposited in the dermis. Deposition of immunoglobulins and complements were also observed at the dermo‐epidermal junction and on the vascular walls. Some immunological processes which may stimulate the synthesis of acid mucopolysaccharides in the dermis were sugges

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1989.tb01283.x

年代:1989

数据来源: WILEY

摘要:

AbstractA male adult patient with discoid lupus erythematosus (DLE) developed lupus erythematosus profundus. We decided to treat him with oral administration of dapsone, which proved to be very effective.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1989.tb01284.x

年代:1989

数据来源: WILEY

摘要:

AbstractA 36‐year‐old woman visited our hospital with a five month history of persistent pustulation, crusting, and alopecia on the vertex of the scalp. No pathological organisms were isolated from the lesions. Histological examination revealed non‐specific changes of chronic inflammation with destroyed follicles. Antibiotic therapy produced no response, but steroid therapy was effective. From these observations, a diagnosis of erosive pustular dermatosis of the scalp (EPDS), as described by Pye et al., was made. The patient also had Hashimoto's thyroiditis, autoimmune hepatitis, and Takayasu's aortitis. The laboratory studies revealed an increased erythrocyte sedimentation rate, C‐reactive protein 3+, hypergammaglobulinemia, and various auto‐antibodies, suggesting the possibility of a pathogenesis common to both this dermatosis and the autoimmune

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1989.tb01285.x

年代:1989

数据来源: WILEY

摘要:

AbstractA 5‐year‐old Japanese girl developed follicular pustules with erythematous plaques on the lower extremities. The histopathology was compatible with eosinophilic pustular folliculitis (EPF). However, the clinical findings were not typical of EPF; the pustules did not appear in crops, the pustules became ulcers, and the skin lesions appeared exclusively on non‐seborrheic

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1989.tb01286.x

年代:1989

数据来源: WILEY