摘要:

AbstractFecal, urinary and erythrocyte porphyrin analyses using the solvent extraction method were performed in 26 patients with porphyria cutanea tarda (PCT) and 144 normal controls. The levels of fecal uroporphyrin (UP) and coproporphyrin (CP) were markedly increased in the PCT group, especially in comparison with the protoporphyrin (PP) level. The values of the UP/PP and CP/PP ratios in the feces were also elevated over those in the control group. It appears that fecal porphyrin excretion is basically similar to urinary porphyrin excretion in PCT. The analysis of fecal porphyrins and the observation of fecal UP/PP and CP/PP ratios may be helpful in the biochemical diagnosis of PCT. In particular, the elevation of CP/PP ratio is characteristic of PCT.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1987.tb03581.x

年代:1987

数据来源: WILEY

摘要:

AbstractEffect of human fibroblast interferon (IFN‐β) on normal human monocyte activation induced by the monocyte activating factor found in sarcoidosis sera was studied. Spreading was used as one indicator of monocyte activation. IFN‐β was shown to inhibit spreading of normal human monocytes induced by the activating factor. The inhibitory activity of IFN‐β against monocyte spreading was adsorbed with monoclonal antibody to human IFN‐β‐Sepharose beads. Increases in phagocytosis and glucose consumption of normal human monocytes induced by the activating factor was also inhibited by IFN‐β. Recombinant IFN‐β also showed

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1987.tb03582.x

年代:1987

数据来源: WILEY

摘要:

AbstractXanthoma was induced by intradermal dextran sulfate (DS) injections into diet‐induced hypercholesterolemic rabbits (HCR) and a Watanabe heritable hyperlipidemic (WHHL) rabbit. The number of infiltrating foam cells and the accumulation of cholesteryl esters, especially cholesteryl oleate, were more remarkable in a HCR with extremely high serum cholesterol level (1,176 mg/100 ml) than in the WHHL rabbit (serum cholesterol level of 327 mg/100 ml), but the number of infiltrating foam cells and the accumulation of cholesteryl esters were similar in a HCR with a serum cholesterol level of about 300 mg/100 ml and the WHHL rabbit. Apparently, the infiltration of foam cells and their accumulations of cholesteryl esters were correlated with serum total cholesterol level. Cholesterol esterification was stimulated and cholesterol synthesis was suppressed in the histiocyte infiltrating dermis incubated with very low density lipoprotein (VLDL), intermediate density lipoprotein (IDL) and low density lipoprotein (LDL) from either the HCR or WHHL rabbit. But supplements of the LDL from normolipemic rabbits (NLR) did not strongly stimulate cholesterol esterification and scarcely suppressed cholesterol synthesis. This suggests that the lipoproteins of the HCR and WHHL rabbit are more easily taken up by dermal histiocytes than the LDL of the NLR. Among the separate lipoprotein classes of the two distinct types of hypercholesterolemic rabbits, there were no significant differences in cholesterol esterification stimulation or cholesterol synthesis suppressio

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1987.tb03583.x

年代:1987

数据来源: WILEY

摘要:

AbstractThe effects of La3+on the distribution and organization of actin in human keratinocytes were examined with rhodamine‐phalloidin staining. At 0.2 and 0.02 mM concentrations, La3+induced redistribution and organization of actin. Thick bundles of actin filaments appeared, as did fine ripple‐like short bundles and polygonal structures. Thick, ribbon‐like assemblages of actin were crescent‐shaped or semi‐circular. These changes were quite similar to those induced by TPA. The effects of La3+were discussed with referenc

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1987.tb03584.x

年代:1987

数据来源: WILEY

摘要:

AbstractFour monoclonal antibodies recognizing antigenic determinants on the nuclei of cultured mammalian cells were isolated. Immunofluorescence studies using these antibodies demonstrated three immunofluorescence patterns of the nucleus; fluorescence staining on the nuclear envelope (antibody 266), on the nucleus (antibody 182, 261) and on both the nucleus and nuclear envelope during the cell cycle (antibody 108). Antibody 108 stained cytoplasmic vesicles of interphase cells. When using mitotic chromosomes, antibody 108 reacted with the perichromosomal region, while antibodies 182 and 261 stained intrachromosomal substance. Immunoblot analysis showed that monoclonal antibody 108 recognized a protein of molecular weight 40K in both the cytoplasm and the perichromosomal region. The molecular weight of the antigen which reacted with antibody 182 was 37K

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1987.tb03585.x

年代:1987

数据来源: WILEY

摘要:

AbstractWe investigated the ultrastructural distribution pattern ofP97 antigen on the cell surface of cultured human melanoma cells with ferritin‐labelled antibody and confirmed the sialic acid nature of the epitope by use of neuraminidase and wheat germ agglutinin, a lectin which binds specifically to sialic acid.At the ultrastructural level, the melanoma‐associated antigen, p97, exists mainly as a solitary element on the cell surface of the cultured human melanoma cell. In small areas, however, the antigen assembles to form small clusters. Our ultrastructural study supports the thesis that the epitope of p97a consists of sialic a

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1987.tb03586.x

年代:1987

数据来源: WILEY

摘要:

AbstractThe cellular composition of the so‐called “sclerosing haemangioma” (or histiocytofibroma‐HF) of the skin has long been controversial and is still uncertain. Histiocytes, fibroblasts and endothelial cells have been considered as the cell‐type of origin of these lesions. In order to assess the alleged endothelial derivation of HF cells, we performed an immunohistochemical study of 22 cases of HF of the skin using a polyclonal antibody to factor VIII‐related antigen (F VIII RA) and an avidin‐biotin‐peroxidase technique on formalin‐fixed, paraffin‐embedded sections. F VIII RA distinctly decorated the small vessels around and inside the tumours, but HF cells themselves were negative. This result, which agrees with previous enzyme‐histochemical studies, speaks against the endotheli

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1987.tb03587.x

年代:1987

数据来源: WILEY

摘要:

AbstractOne case of extramammary Paget's disease was studied by ultrastructural and morphometrical methods to clarify whether or not Paget cells change qualitatively or quantitatively in passing through the epidermis. Paget cells in living layers were observed as large cells with abundant clear cytoplasm, large nuclei, and prominent nucleoli. The cytoplasm was enriched with well developed rough‐surfaced endoplasmic reticulum, mitochondria, free ribosomes, and glycogen particles. In contrast, filamentous structures were not developed. Paget cells are connected to each other and adjacent keratinocytes by small desmosomes.In contrast, in the horny layer, Paget cells contained shrunken, flattened nuclei. Cell organelles had degenerated and large lipid droplets were present in the cytoplasm. As disruption of the desmosomes took place, intercellular spaces between Paget cells and keratinocytes widened. Based on the morphometrical analysis by a sonic digitizer computer system, there were statistically significant decreases in areas and diameters of Paget cells between the horny layer and the living cell layers, including the basal, spinous and granular layers.In conclusion, it is suggested that Paget cells change qualitatively and quantitatively after they move into the horny layer from the living layer

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1987.tb03588.x

年代:1987

数据来源: WILEY

摘要:

AbstractEpidermal adenylate cyclase systems following dispase treatment were investigated. Dispase is a bacterial neutral protease obtained fromBacillus polymyxa. Following the treatment with dispase, the epidermal sheet is easily peeled off the dermis. Dispase‐treated pure epidermal sheets were shown to contain three major (beta‐adrenergic‐, adenosine‐, and histamine‐) receptor adenylate cyclase systems. Without phosphodiesterase inhibitors, the intracellular cyclic AMP (cAMP) level reached the maximal level at 3 min. This effect was markedly enhanced by the addition of cAMP phosphodiesterase inhibitor. Among these epidermal adenylate cyclase systems, the most marked cAMP accumulation was observed by histamine, followed by adenosine, and then by epinephrine. The separation of epidermis and dermis following dispase treatment revealed that epidermis contained most of the beta‐adrenergic response (87%), whereas the dermis retained a significant proportion of adenosine (26%) and histamine (40%) responses when 0.3 mm thickness skin was studied.Specific antagonists of epinephrine, adenosine, and histamine inhibited the effects of these agents completely. The simultaneous addition of two stimulators into the incubation medium resulted in an additive effect.Beta‐augmentations by hydrocortisone, colchicine, and retinoid all remained in the dispase‐treated pure epidermal sheets, but beta‐augmentations by these drugs were spoiled by trypsin treatment.These results indicate that dispase‐treated pure epidermis contains three major (beta‐adrenergic‐, adenosine‐, and histamine‐) specific and independent receptor adenylate cyclase systems. Dispase is a very useful tool for investigating the metabolism and regulatory system of keratinocytes without any significant damage to epiderma

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1987.tb03589.x

年代:1987

数据来源: WILEY

摘要:

AbstractFollowing a standardized trauma to the clinically uninvolved skin of psoriatic patients, an overresponse of several metabolic processes occurs. In the present study, barrier functions of normal looking skin of psoriatic patients and healthy controls were assessed during 5 weeks following a standardized injury. Surprisingly, recovery rates of both groups proved to be identical. The significance of this finding in the epidermal homeostasis of psoriasis is discussed.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1987.tb03590.x

年代:1987

数据来源: WILEY