ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1976.tb01842.x

年代:1976

数据来源: WILEY

摘要:

ABSTRACTA unique glycoprotein has been isolated from cultured human embryonic fibroblasts and their culture medium. Analysis showed a composition of 17.5 γ/mg uronic acid, 40 γ/mg hexosamine, and 93.5 γ/mg sialic acid. Gas chromatography revealed fucose, mannose, galactose, and glucose. Ten percent acrylamide 0.1% sodium sulfate acrylamide disc electrophorisis of the glycoprotein after treatment with 6 M urea showed a RF ratio of 0.3 and a molecular weight of about 95,000. The protein content was 454 γ/mg and amino acid analysis revealed high contents of aspartic acid, threonine, glutamic acid, alanine, and leucine.The existence of acidic glycoproteins in human fibroblasts suggests an association with collagen fibril maturation and may be of etiologic and diagnostic significance in connective tissue disease of the s

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1976.tb01843.x

年代:1976

数据来源: WILEY

摘要:

ABSTRACTAcidic glycosaminoglycans (GAG) prepared from scar and keloid, was assayed for separation and quantitative measurement with the paper chromatographic characteristic of the unsaturated disaccharide unit after chondroitinase digestion. The following results were obtained:1)It was found that the total amount of GAG in scars was greatly increased, especially in earlier phases, in comparison with that of normal tissue, and it gradually decreased with time. Even in different phases of scar, a decrease of hyaluronic acid (HA) was commonly observed in contrast to an increase in sulfated GAG,i.e.dermatan sulfate (DS) in particular amoung constituents.2)A similar behavior of GAG to their mentioned above was observed in keloid specimens.3)Any striking difference of GAG constituents between the scar in the hypertrophic phase and the keloid remained indistinguishable.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1976.tb01844.x

年代:1976

数据来源: WILEY

摘要:

ABSTRACTUsing horseradish peroxidase (HRP) as antigen, reversed passive Arthus reactions were carried out on the skin of rabbits, in which HRP‐anti HRP immune deposits were observed as brownish granules in sections treated with diaminobenzidine and peroxide. Some immune deposits, presumably insoluble immune complexes, were found to be taken up by neutrophilic leukocytes in the subepidermal papillary layer, then pushed up into the epidermal layer and subsequently eliminated from the epidermal surface as small crusts.In the course of this trans‐epidermal elimination, intra‐epidermal and subcorneal micro‐abscesses were observed. Most of the neutrophilic leukocytes in the micro‐abscesses were shown to contain peroxidase‐positive granules. The trans‐epidermal elimination of immune deposits might represent a way of biological clearance of immune deposits occurring in the sub‐epidermal papillary layer or withi

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1976.tb01845.x

年代:1976

数据来源: WILEY

摘要:

ABSTRACTUltrastructural localization of pemphigus autoantibodies has been studied, using the periodatelysine‐paraformaldehyde fixation method of McLean et al.The deposition of pemphigus autoantibodies was always found in the intercellular spaces of the epidermis, but not observed between the basal surface of basal cells and the basal lamina. Within desmosomes, a linear deposition of antibodies was also found, coresponding to the intermediate dense layer of Odlan

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1976.tb01846.x

年代:1976

数据来源: WILEY

摘要:

ABSTRACTWe have presented here an unusual tumor of the hair follicle which did not correspond exactly to any hitherto described hair follicle tumor. This tumor was characterized by an intimate stromal‐epithelial interaction, unmistakable morphodifferentiation towards hair follicle, formation of hair‐like structures and pluripotential adenomatous deviation. In 1962 Headington and French described a tumor to which they gave the noncommital term “trichogenic adnexal tumor”. They thought it was a hair matrix neoplasm in which morphodifferentiation towards hair follicle is distinct and is induced by intimate stromal‐epithelial relationship. In this sense our tumor is considered to be in the category of trichogenic adexal tumor, or at least its intimate relative, although the tumor presented here was better organized with a more complicated adenomatous deviation. It will surely be important as another example for further analysis of hair follic

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1976.tb01847.x

年代:1976

数据来源: WILEY

摘要:

ABSTRACTIndirect immunofluorescent test was used to study the clinical significance of antinuclear antibodies (ANA) in 116 systemic lupus erythematosus (SLE) patients. It was found that ANA was present in 96.5% of SLE patients and that their presence could serve as an important laboratory characteristics. A titer of 160 or more could also serve as suggestive evidence for SLE. The chief morphological appearance of ANA was peripheral pattern and homogenous pattern. The peripheral pattern was associated with high titer. A change of titer and pattern were interrelated. A change from homogenous to peripheral pattern indicated a downhill course of the disease, and a change from peripheral to homogenous pattern showed clinical improvement of the disease.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1976.tb01848.x

年代:1976

数据来源: WILEY

摘要:

ABSTRACTA direct immunofluorescent test was used to demonstrate the in vivo deposits of immunoglobulin (Ig) and complement in the skin of 30 patients with systemic lupus erythematosus (SLE). These deposits were demonstrated in all skin lesions and in 70% of apparently normal skin of patients with lupus nephritis. On the contrary, they were seldom found in seemingly normal skin of patients without nephritis, though they could be seen in skin lesions. There was significant difference between these two groups and as well as an intimate relation of the deposits to clinical severity. The fixed Ig and/or complement could be found in a band arrangement in the basement membrane zone (BMZ) area, nuclear areas of epidermal and/or dermal cells or both BMZ area and nuclear areas.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1976.tb01849.x

年代:1976

数据来源: WILEY

摘要:

ABSTRACTDuring the last years a relative high incidence of recurrent herpes simplex genitalis has been observed in Jakarta. A total number of 58 adult male and female patients suffering from herpes progenitalis and of herpes on the mons veneris, gluteal region or thighs were treated with “Lupidon G.”*“Lupidon G” is a HSV (herpes simplex virus) type 2‐antigen, to be injected subcutaneously according to a fixed treatment schedule. The first course consists of 12 subsequent weekly injections (1st–3rd month), followed by a second course of 8 fortnightly injections (4th–5th month). This paper will discuss the factors contributing to proneness of individuals to the disease, the average duration of the herpes, the prior treatment, frequency of the relapses and the coincidence with other diseases.Twelve patients terminated before completion of the course of treatment or did not come back for control. In 46 patients a follow‐up during 6–12 months revealed the following clinical results: excellent 34.8% (16 cases), good 52.2% (24 cases), and inefficacious 13.0% (6 cases), all after the first course. Two patients of the last group were cured after a second course of treatment. Trivial side‐effects occured on the site of injection i

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1976.tb01850.x

年代:1976

数据来源: WILEY

摘要:

ABSTRACTThe occurrence of antinuclear antibodies (ANA) in eluates of lymphoid cells and polymorphonuclear leukocytes obtained in seven untreated psoriasis patients could be demonstrated by means of the indirect immunofluorescence technique.These antibodies appear to be non‐species‐specific and are mainly directed against the nuclei of the basal cell layer of uninvolved skin, but also ANA directed against the nuclei of epidermal and dermal cells have been encountered.Moreover a significant decrease of circulating lymphoid cells forming rosettes with sheep erythrocytes have been found in the patients studied, suggesting a diminution of the T‐cell control.The immunological mechanisms in psoriasis may therefore be looked upon as being the result of a lack of certain T‐cell suppressor genes facilitating the recognition of nuclear basal cell self‐antigens and a subsequent production of multiclonal anti‐basal cell nuclear antibodies. These antibodies may interfere with the function of membrane bound enzymes which serve as a regulatory epidermal control mechanism.Various exogenous and endogenous factors may help to initiate the immune response leading to a cell‐mediated immune response at the onset of a lesion and an Arthus type reaction located in the upper layers of the epidermis at a later stage of the disease.Consequently the course of events in psoriasis is a self perpetuating inflamm

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1976.tb01851.x

年代:1976

数据来源: WILEY