摘要:

ABSTRACTCombination chemotherapy (DTIC and VCR plus a new derivative of nitrosourea, ACNU) with a new immunoadjuvant of OK432 were evaluated as treatment for disseminated malignant melanoma.Eight subjects with this chemoimmunotherapy showed a significant increase in survival rate (p=0.037 at 8 months), when compared with 11 subjects on other therapy. The regimens were minimally myelo‐suppressive and toxic to liver functions. Cellular immunity with specific mitogens of autologous and allogeneic melanoma cells (MLTC) and non‐specific mitogens of PHA, Con A and PWM was well preserved. Cell‐mediated cytotoxicity against autologous melanoma cells appeared to be stimulated in a way similar to that which occurs with BCG immunoadjuvant th

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1981.tb02544.x

年代:1981

数据来源: WILEY

摘要:

ABSTRACTIn the organ culture of adult human skin, long‐term preservation of cultured epidermis was obtained when the culture temperature was shifted from 37°C to 32°C. The epidermal cell kinetics were compared for both culture temperatures using the findings of mitotic rate, mitotic distribution and autoradiographic labelling index with tritiated‐thymidine (3H‐TdR). As a result, it appeared that a lowered culture temperature controlled the cell cyclein vitroto maintain long‐term, active epidermal prol

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1981.tb02545.x

年代:1981

数据来源: WILEY

摘要:

ABSTRACTApproximately 1250 cases of strawberry mark were encountered during a 16‐year period. The lesions were classified into three major types, (1) plaque type (d), (2) tumor type (Ds, DS, dS) and (3) subcutaneous type (S). The frequency of each clinical type (d, Ds, DS, dS, S) was studied for 1000 lesions. The plaque type was the first in frequency (57%) and the tumor type was the next (40%). The subcutaneous type was much less frequent (3%). The tendency toward spontaneous regression was studied for 308 lesions in 255 cases, which revealed differences in the natural course among clinical types. Lesions of the plaque and subcutaneous types regress with good end results. Those of the tumor type often regress incompletely. The prognosis of a lesion can be predicted fairly well once its clinical type is known. The clinical features of residual lesions have been briefly described. They are telangiectasia, skin redundancy with wrinkles, and protuberance. Histological investigation of 65 lesions in 62 cases including residual lesions showed hyaline degeneration in capillary walls. The pathological aspects of spontaneous regression have also been discusse

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1981.tb02546.x

年代:1981

数据来源: WILEY

摘要:

ABSTRACTSeveral distinct morphological abnormalities of hair were discussed. Most of congenital hair defects appear to be related to anatomic (defective cuticle formation) and biochemical (reduced sulfur content) properties.The major structural hair abnormalities include defects in the cuticle, cortex and medulla. Our studies have revealed that there were defects of the cuticle in each case of a major structural hair defect.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1981.tb02547.x

年代:1981

数据来源: WILEY

摘要:

ABSTRACTThe levels of 5‐S‐cysteinyldopa (5‐SCD), 3,4‐dihydroxy‐phenylalanine (DOPA), and dopamine (DA) in the urine were determined by means of high‐performance liquid chromatography (HPLC) and fluorometry. There was a definite correlation between the data obtained by means of HPLC and those by fluorometry, suggesting that both methods are favorable for determining the levels of 5‐SCD, DOPA, and DA in the urine. However, HPLC is more useful than fluorometry for the following reasons: HPLC is easy for clinical practitioners to use; each substance shows good recovery; the values of 5‐SCD, DOPA, and DA can be determined in a single chro

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1981.tb02548.x

年代:1981

数据来源: WILEY

摘要:

ABSTRACTWhen café au lait spots or pigmented nevi were combined with Mongolian spot lesions, whitish haloes were observed in the areas surrounding these café au lait spots and pigmented nevi. The pathogenesis of this halo‐like phenomenon was investigated. The materials were two Japanese girls who had Mongolian spots present at birth over the sacrum and buttocks. In case 1, an eight‐month‐old, café au lait spots and in case 2, a six‐month‐old, brownish black pigmented spots appeared about one month after birth and gradually increased in size and number on the trunk and extremities. In the café au lait spot and pigmented nevus (histologically compound type) lesions and their boundary areas, a whitish halo‐like zone, dermal melanocytes were small and round, as compared with those of surrounding Mongolian spots, although only slightly decreased in number. The characteristic bipolar dendrites were almost imperceptible. The dopa reaction of dermal melanocytes was also decreased. In the boundary halo‐like areas in both cases, epidermal melanin granules and dopa‐positive melanocytes were almost identical to those of the surrounding Mongolian spot.Mongolian spots were already present at birth in both cases, but the café au lait spots and pigmented nevi definitely appeared to arise on these Mongolian spot after birth. The Mongolian spots seemed to disappear at the sites of café au lait spots, pigmented nevi and boundary areas, perhaps a kind o

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1981.tb02549.x

年代:1981

数据来源: WILEY

摘要:

ABSTRACTUltrastructural studies of vitiligo with inflammatory raised borders were performed. A 41‐year‐old Japanese female had developed vitiligo on the back and cervical region about six months previously and it gradually increased. Slightly elevated red borders were observed on the periphery of some of the vitiligo lesions. Electron microscopic findings showed a few melanocytes with decreased and irregular melanosomes in the basal layer and dissociation of keratinocytes in the spinous layers. Melanosomes in keratinocytes were markedly decreased. Lymphocytes were frequently observed intraepidermally, and the process by which lymphocytes passed to the basal lamina from the dermis was also observed. Lymphocytes were seen in apposition to some melanocytes in the basal layer. These melanocytes, as a result, may degenerate. The formation of many vacuoles in the cytoplasm was observed. Many typical Langerhans cells with many racket bodies were observed in the upper spinous layers. On the other hand, in the basal layer, Langerhans cells with only a few racket bodies were generally observed. These Langerhans cells were also frequently in contact with lymphocytes.The cause of this inflammatory reaction with a raised border is not known. However, these ultrastructural findings firmly suggested a relationship to some type of immunological mechanism for this occurrence of vitil

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1981.tb02550.x

年代:1981

数据来源: WILEY

摘要:

ABSTRACTIn an attempt to clarify the role of prostaglandins in the pathogenesis of psoriasis, indomethacin, a potent inhibitor of prostaglandin synthesis, was administered orally to 6 psoriatic patients and topically to 5 patients. In all cases remarkable exacerbation of pre‐existing lesions was observed within 1 to 4 weeks. The possible mechanism of the clinical exacerbation was discussed with particular reference to arachidonic acid‐prostaglandin sys

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1981.tb02551.x

年代:1981

数据来源: WILEY

摘要:

ABSTRACTMembraneous fractions were isolated from human stratum corneum. The fractions obtained after frequent reextraction with urea and 2 ME at pH 9.0 (A), and further digestion of (A) by trypsin (B) were compared using electron microscopy and amino acid analysis. Low molecular weight proteins or amino peptides were solubilized from fraction (A) following digestion with trypsin, chymotrypsin and pronase P. The amounts of solubilized material showed a plateau after 30 minutes of digestion with trypsin. A small fraction of contaminants thought to be the fibrous and interfibrous materials were still observed in Fraction (A) by electron‐microscopy and SDS gel electrophoresis, but no remarkable contaminants were observed in Fraction (B), which was composed of trilaminar membraneous parts including the marginal band. Fraction (A) contained 43 half cystine residues per 1000 amino acid residues, whereas Fraction (B) contained 81 half cystine residues per 1000 amino acid residue

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1981.tb02552.x

年代:1981

数据来源: WILEY

摘要:

ABSTRACTUsing light and electron microscopy, we studied five cases of tyrosinase positive albinism, with special reference to melanization of the hair‐bulbs before and after incubation with L‐tyrosine. Electron microscopic autoradiographs of the hair‐bulbs after incubation with3H‐L‐tyrosine were also investigated. As a result, it was found that three cases could be categorized as ordinary tyrosinase positive albinos, but the other two could not. It was concluded that tyrosinase positive albinism might not be a homogeneous disease, but a disease which can be divided into several s

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1981.tb02553.x

年代:1981

数据来源: WILEY