摘要:

The dihydrofolate reductase activity has been studied cytochemically in various haematological diseases. The variation between normal controls, Hodgkin's disease, myeloma, polycythaemia vera, chronic lymphocytic leukaemia and chronic myeloid leukaemia was not significant, comparing the same type of cells.In acute myeloid leukaemia and acute lymphoblastic leukaemia the blast cells were weakly positive or negative. This finding is very interesting as the blast cells are capable of division. Probably the dihydrofolate reductase appears in the blast cells in some stage of mitosis.Lymphocytes stimulated by phytohaemagglutinin showed increased enzyme activity compared with normal non‐stimulated lymphocytes. The ‘blast like’ cells were more strongly positive than the blast cells of leukaemic patients. The patients with acute lymphoblastic leukaemia or acute myeloid leukaemia treated with methotrexate showed increased dihydrofolate reductase activity cytochemi

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1977.tb02712.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

Cell kinetics have been studied at several periods in a patient with typical cyclic neutropenia. Peripheral blood granulocyte labelling showed an increased destruction in the preleucopenic phase. Bone marrow colony‐forming cells and blood leucocyte colony forming activity were normal or above the normal range. The major abnormalities were found in sequential studies of bone marrow proliferation measured by ‘in vitro’3H‐thymidine flash labelling. A fall in the labelling index of promyelocytes was observed in the second part of the cycle. Incubation of normal human bone marrow cells with patient's granulocytes showed a marked decrease in3H‐thymidine incorporation, compared with incubation with the same number of normal granulocytes. Human cyclic neutropenia seems to be due to a factor secreted by abnormal polymorphonuclears inhibiting myeloid prol

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1977.tb02713.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

When stained and unstained sections of hypersiderotic marrow fragments are examined in the electron microscope, some of the ferritin molecules present between a variable proportion of the erythroblasts are found to be aligned more or less transversely. These linearly arranged ferritin molecules are particularly evident in regions in which the opposing cell membranes have been sectioned obliquely and may be confused with the septa‐containing intercellular junctions characteristically present between some types of invertebrate epithelial cell

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1977.tb02714.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

Cytochalasin B (CB), 5 μg/ml (= 1.0 times 10‐5M), inhibited the oxalate‐induced radial segmentation of the nuclei of lymphocytes and monocytes from peripheral blood. The median inhibition was 60 %. The oxalate‐induced radial segmentation (RS) is thought to be due to a microtubule‐dependent contraction of the intermitotic residue of the mitotic apparatus around the nucleus. CB is thought to inhibit cell locomotion and cytokinesis by a centripetal contraction of the membrane‐associated contractile cell layer without subsequent relaxation. It is thus suggested that the CB inhibition of the oxalate‐induced RS was due to a spatial interference of the CB‐induced contraction with the formatio

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1977.tb02715.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

A total of 19 cases of chronic refractory anaemia underwent simultaneous in vivo erythrokinetic study and in vitro bone marrow culture. They were followed up clinically for at least 2 years. Good correlation has been found between erythrokinetic data (simultaneous quantitative and qualitative disorders of erythroblastic proliferation in preleukaemic states; pure qualitative disorders in primary sideroblastic anaemia) and the results of culture of granulocyte precursors in the bone marrow (small number of colonies, reduced size of colonies in preleukaemic states; normal number and growth in non malignant refractory anaemia). It would seem thus that both examinations are of practical interest in clinical haematology, making it possible to foresee the malignant evolution of some refractory anaemias.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1977.tb02716.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

100 patients showing a significant number of stomatocytes were identified from the microscopic examination of 4291 peripheral blood films. The distinctive red cell appearance was most commonly found in patients with neoplastic, cardiovascular or hepatobiliary disease including acute alcoholism and during therapy with certain drugs some of which are known to be stomatocytogenic in vitro.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1977.tb02717.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

A deficient erythrocyte pyruvate kinase observed in a patient with congenital non‐spherocytic anaemia was characterized by the following properties: very low activity in haemolysates, decreased thermal stability, slightly increased urea denaturation, high affinity for PEP, poor FDP activation, normal ATP inhibition, decreased affinity for ADP, normal pH of optimal activity, and presence of an abnormal slow‐moving component in this layer polyacrylamide gel electrophoresis. The patient was probably double heterozygous for two different deficient mutants of erythrocyte pyruvate kin

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1977.tb02718.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

100 cases of autoimmune haemolytic anaemias (AIHA) in Thailand were analyzed. Clinical and immunological aspects of the disease were compared with those described in other parts of the world. Racial differences, especially between Thais and Caucasian, were emphasized. Thai patients show the following distinctions: (1) AIHA tends to occur in people of a younger age group, predominantly in females, (2) a rare occurrence of AIHA of cold‐antibody type, an incidence which significantly differs from that in Caucasians, and (3) the autoantibodies consist of more than one immunoglobulin class in more than 50 % of cases. Possible factors that might contribute to these differences were discusse

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1977.tb02719.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

The effect of an acute myocardial ischaemia on circulating platelet aggregates (CPA) in the coronary sinus and aorta was studied in open chest dogs. Even before induction of myocardial ischaemia significantly more CPA were found in the coronary sinus than in the aorta. Acute myocardial ischaemia produced by a non‐thrombotic coronary artery occlusion increased CPA in coronary sinus, but not in aorta.Administration of acetylsalicylic acid (ASA) reduced the extent of the myocardial ischaemia as evidenced by reduced ST‐segment elevations in epicardial ECG. Before induction of myocardial ischaemia, ASA significantly reduced CPA in coronary sinus, but no significant effect was observed during myocardial ischaemia. In the aorta no effect of ASA on CPA was fo

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1977.tb02720.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

A case of polycythaemia secondary to uterine fibromyoma is reported. Polycythaemia disappeared after hysterectomy. Erythropoietic activity was detected in the cyst fluid of the tumour. Plasma and urine contained no erythropoietic factor. Polycythaemia was related to erythropoietinlike material produced by the tumour.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1977.tb02721.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY