摘要:

Human endothelial cells were isolated from the umbilical cord vein by collagenase treatment and cultured for periods up to 6 weeks. The cultured cells were identified as endothelium by cell morphology and growth pattern, the presence of Weibel‐Palade bodies, and their ability to stimulate allogeneic lymphocytes (Hirschberg et al 1974). Cultured fibroblast‐like cells derived from the umbilical cord were clearly different in all three respects. Approximately one third of the primary endothelial cultures showed clear evidence of proliferation during the first 3–4 days in culture as judged by cell counting. Replicating ability in a culture was correlated with cell density at the time of seeding. Autoradiography of endothelial cells after exposure to3H‐thymidine showed a 30‐fold increase in nuclear labelling from day 1 to day 3 in culture. The endothelial cells have so far been subcultured th

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb02422.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

A new case of erythrocyte pyruvate kinase deficiency was described. – A 9‐year‐old male patient was hospitalized because of anaemia, jaundice and splenomegaly. Diagnosis was made primarily on the basis of the erythrocyte enzyme studies. Because the pyruvate kinase of this patient demonstrated certain different characteristics from the other variants described previously, it was tentatively designated PK ‘Ma

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb02423.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

The case of a 27‐year‐old woman with pancytopenia, revealing acute monocytic leukaemia and haemolytic anaemia, is described in detail. The underlying cause for the red cell destruction was found to be a pyruvate kinase (PK) instability. Further investigation into three generations of her family (n = 12) disclosed a hereditary PK instability. This was proven by performing biochemical studies to elucidate mutants representing a structurally defective enzyme. Since conversions of pancytopenia with acquired red cell enzyme deficiency into leukaemia have been described, our observation emphasizes that hereditary red cell enzymopathy might also be associated with adult acute leukae

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb02424.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

Spleen size, stomatocytosis, macrothrombocytopenia, haemoglobin level, white cell count, and abdominal pain episodes were assessed in a coded study of healthy Mediterranean immigrants to Australia. Spleen size was estimated from a length measurement, L, on a standardized plain abdominal radiograph and expressed both as spleen weight and as a spleen length index, L/l/BSA; the platelet count and size parameters were determined electronically and the presence of stomatocytes was evaluated in stained blood films. In relation to 16 Northern European control women 12 of 25 Mediterranean women had radiographic splenomegaly, 10 had macrothrombocytopenia, 9 had stomatocytosis, but none had episodes of abdominal pain. The median spleen weights of the two groups were estimated as 157 and 247 g with ranges from percentile 2.3 to 97.7 of 75 to 328 and 112 to 669 g. Within the Mediterranean group splenomegaly correlated with macrothrombocytopenia (P<0.001) but not with stomatocytosis, haemoglobin values or white cell counts. Thus, mild splenomegaly may be expected in Mediterranean macrothrombocytopenia, Mediterranean stomatocytosis appears unrelated, and all of these apparently benign anomalies may be incidental findings in patients from the Italian and Balkan peninsulas.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb02425.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

A case of chronic cold‐haemagglutinin disease is reported in which an agglutinin apparently carrying two specificities was present. At first, specificity was anti‐not‐I and anti‐I respectively: in the course of time anti‐I was replaced by anti‐A1. Both anti‐I and anti‐A1could be demonstrated at room temperature only while specificity was anti‐not‐I at 4°C. The antibody displayed haemolytic activity at room temperature and gave stronger reactions when treated biphasically. The same pattern of specificity was apparent in haemolysis tests, i.e. anti‐not‐I in the biphasical reaction 4°C ° 37°C and first anti‐I and later anti‐A1at room temperature and at 22°C → 37°C. Anti‐A1was not neutralized by A bloodgroup substance of

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb02426.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

An N‐terminal fragment of human prothrombin (m.w. 19,600) had no detectable effect on the aggregation of platelets by ADP, collagen or thrombi

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb02427.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

A potent inhibitor of factor V was discovered in a middle aged woman shortly after streptomycin therapy. The patient suffered from severe bleeding over a period of 10 days and the inhibitor was demonstrable for 6 weeks. By use of specific antisera the anticoagulant was found to be of an IgG nature. In agar electrophoresis the inhibitor mowed with the immunoglobulin fraction and was separable from factor V activity in the pathological plasma although no factor V activity was demonstrable in untreated patient plasma. It is suggested that this method should be used to distinguish between cases of inhibitors in patients with congenital factor V deficiency and cases of transient inhibitors in patients with normal factor V production.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb02428.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

A survey was conducted among 46 pediatric institutions in the United States and Canada to evaluate the presentation, therapy and survival of children with thrombocytopenic purpura (TTP) seen since 1960.TTP is an uncommon disease in the pediatric age group, and the clinical and pathologic findings in adults and children are almost identical. It can be distinguished from haemolytic uraemic syndrome in that it usually occurs in older children, the renal disease is milder, and the central nervous system symptoms appear to be related to vascular occlusive disease, and not to the complications of severely compromised kidneys. Appropriate biopsy specimens which reveal typical widespread hyaline occlusion of arterioles may confirm the diagnosis.Combinations of corticosteroids, splenectomy, and heparin have been used as therapy. Prognosis has improved, and is probably related to improved supportive care.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb02429.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

In order to evaluate a method permitting quantitation of splenic red blood cell destruction, a model of erythrocyte destruction in enlarged spleens was created: Erythrocytes are destroyed in the splenic erythrocyte pool at a constant rate, producing in labelling studies hyperhaemolysis due to random destruction. A mathematical analysis of the model shows that the splenic destruction rate can be calculated with great accuracy from quantitation of the initial excess radioactivity, measured over the spleen during the first days after infusion of51Cr‐labelled autologous erythrocytes.18 patients with splenomegaly (479–4700 g) were investigated. The splenic erythrocyte destruction rate was estimated to be between 0.5–4.4% of the total erythrocyte mass per day, increasing significantly with increasing splenic weight. The results indicate that erythrocyte destruction takes place almost exclusively in the enlarged spleen in cases of predominant splenomegaly without complicating immunohaemo

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb02430.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

Chromosome analyses using the Giemsa banding technique were performed on bone marrow cells in a patient with the association of Hodgkin's disease and acute myeloid leukaemia. All cells had an abnormal karyotype showing an extra chromosome No. 14, loss of one chromosome No. 17 and gain of one chromosome No. 18. These abnormalities are in many respects similar to the karyotype changes of lymphoid cells in malignant lymphomas, suggesting a pathogenetic relationship between the two disorders.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb02431.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY