摘要:

The purpose of this study was to examine the accuracy of iron absorption calculated from the incorporation of radioiron into red cells (RCI), compared to measurement by the whole body counting technique (WBR). RCI of orally administered59Fe, and absorption of59Fe assessed by WBR were measured simultaneously in 53 chronic uraemic patients (16 non‐dialysed, 18 peritoneal dialysed, 19 haemodialysed), 14 renal transplanted patients with normal renal function, and 27 healthy subjects. In the majority of subjects RCI values were lower than corresponding WBR values, with mean red cell59Fe utilization values (RCI/WBR ratio x 100) in the various groups from 78% to 93%. All groups demonstrated significant correlations between RCI and WBR with r values from 0.963 to 0.996 (P<0.001). RCI was higher in patients with reduced marrow iron stores than in patients with adequate iron stores (P<0.001), and was correlated both to plasma transferrin (r = 0.59, P<0.001) and serum ferritin (r = ‐0.88, P<0.001). In all groups there was good accuracy of calculated iron absorption (from RCI) compared to ‘true’ iron absorption (by WBR) with r values from 0.963 to 0.995 (P<0.001). Iron absorption measurement based on red cell incorporation appears to be a practical and accurate alternative to whole body counting, both in healthy subjects and in patients with chronic

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1982.tb00555.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

Ferritin and ferric hydroxide represent two forms of iron which are less available for absorption than that present in the ‘common pool’ of non‐haem dietary iron. In the present study the absorption of iron from these two compounds was compared in 35 multiparous women when fed in water, in maize porridge and in maize porridge containing 100 mg ascorbic acid. The geometric mean absorption for 3 mg ferritin iron was 0.7% and for ferric hydroxide, 2.4%. Comparable figures when fed with maize porridge were 0.4% and 0.4% respectively. When 100 mg ascorbic acid was present in the porridge, absorption was enhanced from both sources, being 12.1% for ferritin and 10.5% for ferric hydroxide. These results indicate that the fraction of iron in ferritin and ferric hydroxide that enters the ‘common pool’ of non‐haem dietary iron is profoundly influenced by the nature of the diet. The greater the concentration of enhancing ligands, the closer does the absorption of iron from these compounds approximate that of the non‐haem diet

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1982.tb00556.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

A 17‐year‐old girl swallowed at least 50 Duroferon duretter®. Each tablet contains ferrous sulphate equivalent to 0.1 g Fe2+. Thus, a total of 5 g Fe2+was ingested. Vitamin K‐dependent coagulation factors dropped within the next hours to very low levels. Thrombotest showed less than 3% of normal coagulation activity 8 h after oral intake. Recovery was uneventful except for laboratory evidence of transient liver damage. The rapidity by which the early coagulation deficiencies developed and the lack of evidence of disseminated intravascular coagulation suggested a direct effect of iron on coagulation factors. In vitro studies confirmed that iron in concentrations that may have been attained in vivo, altered the functional activity of several coagulation factors. Monitoring the vitamin K‐dependent coagulation factors may be a simple and useful parameter in acute iron into

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1982.tb00557.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

Sera from 21 patients with systemic lupus erythematosus (SLE) were tested with a platelet facor 3 (PF3) assay. A control serum (pooled blood group AB serum) was always run in parallel with the test sera. The test and control sera were run in duplicate and for each patient the mean recalcification time was used for the calculation of the test serum: AB serum ratio. Concomitantly, the patients' serum concentrations of circulating immune complexes (CIC) were measured by using the Raji cell radioimmunoassay. 20 healthy hospital employees served as controls. In the latter subjects the mean test serum: AB serum ratio was 0.99 (range 0.92‐1.09), their CIC concentrations being ≤ 16 μg/ml. 7 of the SLE patients had CIC values ≤ 16 μg/ml, and their test serum: AB serum ratios ranged 0.95‐1.09. All the remaining SLE patients had elevated values for CIC. In the total material of SLE there was a highly significant (r= ‐0.87; P<0.001) negative correlation between the test serum: AB serum ratios and the values for CIC. It is widely held that the so‐called PE3 immunoinjury test detects circulating antiplatelet antibodies. The present results, however, strongly suggest that the assay rather or also detects CIC which like antiplatelet antibodies may damage platelets as we and others have shown in prior

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1982.tb00558.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

An unusual case of ‘non‐excretory’ myeloma is described in which, using immunofluorescen‐ce, only ‘\i’ heavy chains were detected in almost all bone marrow plasma‐cell cytoplasm. Cytoplasmic light chains were completely lacking, and neither monoclonal whole immuno‐globulin (Ig) nor free heavy nor light chains were detected in serum and urine, although the clinical and morphological features showed the classical pattern of myeloma. The possible mechanism which could play a role in the disturbance of the Ig‐chain secretion observed in this

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1982.tb00559.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

Triton X‐100 resistant isozymes of amino acid naphthylamidase were studied by polyacryla‐mide gel electrophoresis, gel filtration and affinity chromatography in samples from 12 human haematopoietic cell lines, 8 leukaemic patients and 4 healthy persons. Affinity to WGA‐Sepharose seemed to be correlated to differentiation in the myeloid series, indicating differences in the N‐acetyl‐glucose‐amine content of the enzyme molecules in mature and immature cells. A high carbohydrate content might be characteristic for mature myeloid cells. By trypsin digestion of intact cells and analysis of released enzyme activities into the medium new information on isozyme expression of amino acid naphthylamidase in the granulocyte‐monocyte lineages coul

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1982.tb00560.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

2 cases surviving acute fatty liver of pregnancy are reported. Both cases had signs of disseminated intravascular coagulation, and extremely low plasma concentration of antithrombin III. One of the women received antithrombin III concentrate. The rationale of this therapy is discussed.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1982.tb00561.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

The function of111In‐labelled platelets has been assessed by collagen‐induced aggregation of platelets in samples of whole blood. The blood samples were drawn after injection of autologous111In‐labelled platelets in 19 subjects undergoing platelet kinetic studies. It was thus possible to measure the aggregability of labelled and unmanipulated platelets simultaneously.111In‐labelled platelets aggregated to the same extent as unmanipulated platelets when tested from 10 min to 24 h after injection of the labelled platelets. The results confirm the assumption that minimal damage is inflicted on the platelets during the isolation and labelling procedures, and support the concept that platelets manipulated in vitro may recover in vivo within a few minutes after rein

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1982.tb00562.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

Subpopulations of human T lymphocytes were analysed by monoclonal antibodies (OKT3, OKT4, OKT8) in healthy controls and in patients with multiple myeloma (MM), Walden‐ström's macroglobulinemia (WM) and benign monoclonal gammopathy (BMG). Lymphocytes forming rosettes with SRBC correlated well with T cells staining in indirect immuno‐fluorescence by OKT3 monoclonal antibodies. The relative and absolute numbers of OKT4+T cells were significantly lower in patients than in controls. Though, the percentage of OKT8+T cells was increased in patients, the total OKT8+cell counts were normal in all patient groups. The ratio between OKT4+and OKT8+lymphocytes was low in the groups of treated MM and of WM patients compared to controls (P<0.001). Moreover, the ratio was lower than the normal range in 27% of BMG and 38% of untreated MM patients. The imbalance between OKT4+and OKT8+T cells in untreated MM was more pronounced in clinical stage III patients than in stage I and II patients. The most pronounced changes were noted in treated MM patients. The significance of the altered T cell subsets in monoclonal gammopathies with regard to polyclonal and tumor B cell regulation remains to be establi

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1982.tb00563.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

A 73‐year‐old patient who had polycythaemia vera (PV), and who 6 years later developed chronic lymphocytic leukaemia (CCL) is described. 2 years after the appearance of CLL, the polycythaemic phase showed a remission following treatment with phlebotomy and busulfan. CLL did not make all the characteristics of PV disappear: leucocyte alkaline phosphatase and fibrosis of the bone marrow remained increased and the erythrocyte sedimentation rate remained low. The abnormal lymphocytes were B‐cells and had the peculiarity that most of their nuclei showed a deep cleft, and some were bilobed. Chromosomal studies of the bone marrow showed the presence of a metacentric chromosome in the C‐group, while the karyotype of the peripheral blood was normal. This case is described because of the rarity of the coexistence of these 2 diseases in the same

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1982.tb00564.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY