摘要:

Both polymorphonuclear (PMN) and mononuclear (MN) leucocytes constitute targets for glucocorticoid hormones. In order to comparatively characterize the earliest steps of steroid action in these cell populations, we investigated the concentrations and specificities of glucocorticoid receptors in purified human PMNs and MNs by a whole‐cell binding assay using (3H)dexamethasone as the ligand. PMNs and MNs were found to contain the same amounts of glucocorticoid receptors (4720 and 4900 receptor sites/cell, respectively). The equilibrium dissociation constant (Kd) of the interaction between the cellular receptor and (3H)dexamethasone was about the same (1 times 10‐8M) in both cell populations. No significant differences in the specificities of the steroid binding sites in PMNs and MNs were found; competition studies revealed the following order of relative binding affinities for a number of compounds: betamethasone>dexamethasone>prednisolone>cortisol>deoxycortico‐sterone = progesterone>aldosterone>testosterone>estradiol‐17β. We conclude that known differences in the sensitivities of PMNs and MNs to glucocorticoids are apparently not caused by differences in the concentrations or characteristics of their glucocorticoid r

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1981.tb00465.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

2 children with Down's syndrome showed severe anaemia, leucocytosis with blastic cells, thrombocytopenia and hepatosplenomegaly. Bone marrow aspirations were near‐dry tap and marrow biopsy revealed primary myelofibrosis with myeloid metaplasia (MMM). Their course was short with a blood picture similar to that of leukaemia. They expired 2 months and 2½ months after diagnosis, respectively. The cases were thought to represent an acute childhood variant of MMM.Cytogenetic study of circulating white cells by 24 h culture without phytohaemagglutinin stimulation revealed aneuploidy in both cases, the first case showing marked aneuploidy with a predominant karyotype of 50, XX,+8, + 19, + 19,+21 and the second case a mosaic of 47,XX,+G/48,XX, + G, + G. The karyotype of phytohaemagglutinin stimulated lymphocytes was 47,XX,+G in both cases.These findings suggest that the abnormal karyotypes are those of circulating blastic cells which are abnormal clones of haematopoietic cells responsible for MMM. In Down's syndrome, MMM might not be so rare as report

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1981.tb00466.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

A 35‐year‐old woman with severe Glanzmann's thrombasthenia became pregnant involuntarily. As a late abortion might cause a bleeding as severe as a delivery, legal abortion was not considered indicated. Treatment with ordinary platelet concentrates was not considered since even HLA‐matched platelets did not enhance the platelet function, measured as the aggregability of transfused platelets. This inability was thought to indicate that the patient might have developed antibodies against one of the glycoproteins deficient in this disease. A delivery per vaginam was then preferred because of the local compressing effect. Spontaneous delivery occurred at the end of the 42nd week of pregnancy. The patient delivered a boy with transient thrombocytopenia, probably due to maternal antibodies. To stimulate the haemostasis, uterine contracting drugs were used in unusually large doses and for a relatively long period of time. Tranexamic acid was also given. No haemorrhagic complications could be observed during the puerp

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1981.tb00467.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

The levels of serum ferritin were investigated in 44 young men during strenuous ranger training courses lasting 4–5 d. These cadets were continuously subjected to heavy physical activities, almost without sleep and with a caloric deficit amounting to 29000–39000 kJ per 24 h. Ferritin concentrations were consistently increased during the courses, and were still significantly elevated 2 d after the end of the courses. The increase of ferritin appeared to be correlated with decreased Hb values and increased total bilirubin. The results indicate that iron released from disintegrated erythrocytes probably causes increased ferritin synthesis in the reticuloendothelic cells and a subsequent release of ferritin in bl

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1981.tb00468.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Cell‐mediated and humoral immunity to herpes simplex virus (HSV), cytomegalovirus (CMV) and varicella zoster virus (VZV) were examined in 37 patients with Hodgkin's disease in continuous long term remission. This group had lower blast‐transformation than a matched control group to all 3 antigens.Patients originally showing B‐symptoms had higher transformation to VZV than those with A‐symptoms. Patients treated with irradiation only had higher transformation than those treated with either chemotherapy or a combination of chemotherapy and irradiation. There was a clear tendency towards lower transformation in patients having been in remission for 2 years or less.Phytohaemagglutinin (PHA) stimulation gave lower response in the patient group than in the control group. Patients with B‐symptoms had lower response than those with A‐symptoms. Interferon production was specially impaired in patients with B‐symptoms. The patient group had higher CF titers against HSV and CMV while the control group had higher titers against VZV. B‐symptom patients had higher titers against VZV than A‐symptom patients.It is concluded that HD patients have impaired immune function many years after discontinuation of therapy, but there are certain differences regarding the in vitro immunity within t

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1981.tb00469.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Antibody‐dependent cellular cytotoxicity (ADCC) by cells from peripheral blood was studied in patients with: chronic lymphocytic leukaemia (CLL), chronic granulocytic leukaemia (CGL), acute lymphoblastic leukaemia (ALL), acute non‐lymphoid leukaemia (ANLL) and compared with that of healthy donors. The ADCC activity in all the types of leukaemia was on the average lower as compared with the control values, being especially low in both CLL and CGL. Further, it has also been shown that the ADCC activity of non‐leukaemic blood cells in ANLL was lower as compared with control values. The latter observation indicates that, at least in ANLL, the reduced ADCC potential of peripheral blood cells is not due to dilution of effector cells by poorly‐ or non‐active leukae

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1981.tb00470.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Lysozyme positivity in lymph nodes from 33 patients with Hodgkin's disease was examined with an immunoperoxidase technique. Positive reactive histiocytes were found in 18 cases; in 7 cases lysozyme was found also in mononuclear Hodgkin cells and in 3 cases in both reactive histiocytes, mononuclear Hodgkin cells and Reed‐Sternberg cells. There was no clear‐cut correlation between cellular lysozyme positivity and such feature as histological type, clinical stage and plasma lysozyme. The findings support the theory that the malignant cell in Hodgkin's disease is derived from a lysozyme producing macrophage. The finding that lysozyme positivity was an inconstant feature may reflect both varying functional status of the macrophages and varying differentiation of Hodgkin cells. Increased plasma lysozyme in Hodgkin's disease may stem from both reactive histiocytes, mononuclear Hodgkin cells and Reed‐Sternberg cells, but the major part is probably contributed by reactive histio

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1981.tb00471.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

A retrospective study of 44 patients presenting with acquired bi‐ or pancytopenia (B/PC) and no evidence of any causal disorder was carried out. 12 patients developed acute non‐lymphoblastic leukaemia after 2–51 months. Compared to the nonleukaemic subjects the preleukaemic patients at the onset of B/PC had significantly higher counts of myeloblasts and other myelopoietic cells in the bone marrow, more cellular marrows due to more dense cellularities of all compartments, higher blood granulocyte and monocyte counts, more pronounced anisocytosis, and in some cases a small number of circulating myeloblasts. A stepwise multiple logistic regression analysis identified 3 combinations of variables displaying significant simultaneous association with subsequent leukaemic transformation. Among these the combination of marrow myeloblast percentage and blood granulocyte count might be applicable to early prospective identification of patients with preleukaemic

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1981.tb00472.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

The absorption from a 3 mg dose of ferrous iron was measured in 250 male subjects. The absorption was related to the log concentration of serum ferritin in 186 subjects of whom 99 were regular blood donors (r = —0.76), and to bone marrow haemosiderin grading in 52 subjects with varying iron status. The purpose was to try and establish a percentage absorption from such a dose that is representative of subjects who are borderline iron deficient. This information is necessary for food iron absorption studies in order (1) to calculate the absorption of iron from the diet at a given iron status and (2) compare the absorption of iron from different meals studied in different groups of subjects by different investigators. The results suggest that an absorption of about 40 % of a 3 mg reference dose of ferrous iron is given in a fasting state, roughly corresponds to the absorption in borderline‐iron‐deficient subjects. The results indicate that this 40% absorption value corresponds to a serum ferritin level of 30 μg/1 and that food iron absorption in a group of subjects should be expressed preferably as the absorption corresponding to a reference‐dose absorption of 40 %, or possibly a serum ferritin level of

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1981.tb00473.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

The amount of radioiron released from rat peritoneal macrophages after phagocytosis of59Fe labelled erythrocytes can be enhanced by addition of desferrioxamine. The effect is dose dependent and the iron chelated by desferrioxamine appears to be at the expense of ferritin. However, desferrioxamine does not appear to chelate iron already incorporated into ferritin. It seems likely that the iron comes from a labile chelatable pool through which the iron from haemoglobin catabolism passes before being incorporated into ferritin. The desferrioxamine appears to enter the macrophage and chelate iron to form ferrioxamine which subsequently leaves the macrophage. In vivo it was not possible to show substantial iron chelaton by desferrioxamine in rats when59Fe labelled non‐viable red cells were injected intravenously. This suggests that in vivo mobilization of reticuloendothelial iron by desferrioxamine may be of limited significanc

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1981.tb00474.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY