摘要:

Myeloperoxidase (MPO) activity of blood granulocytes was estimated in 96 cases of acute myeloid leukaemia (AML), in 35 patients obtaining complete remission and in 14 of these patients during later relapse. As the pretreatment value of MPO activity was the same in patients who died before obtaining remission as in patients obtaining complete remission, determination of MPO‐deficient PMN has no prognostic value with respect to the probability of obtaining complete remission. While half of the untreated patients had increased numbers of MPO‐deficient PMN in the blood, all patients in complete remission had normal MPO activity (P = 0.0002). A normalization of the MPO activity after induction therapy, therefore suggests remission. Positive correlations could be demonstrated between an initially abnormal MPO activity and abnormal activity at relapse as well as between an initially normal MPO activity and normal activity at relapse (P = 0.0004).It is concluded that determination of the % of MPO‐deficient PMN in the blood, may be a useful indicator of complete remission in AML, and in serial determinations during the remission phase also an indicator of threatening re

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1983.tb02128.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

An overdose of CCNU (600 mg over a 15‐d period) was unintentionally ingested by a patient with advanced Hodgkin's disease subjected to combination chemotherapy. A severe bone marrow depression occurred 3 weeks after the start of the CCNU treatment. The nadir of the platelet count was reached after 4 weeks and that of the granulocyte count after 5 weeks. At the nadir of the white blood cell count, colony‐forming cells (CFU‐C) were found in significantly reduced numbers in the bone marrow, and were not found at all in the peripheral blood; the amount of colony‐stimulating activity (CSA) produced by peripheral blood cells was reduced. However, the cells producing CSA recovered earlier than the CFU‐C, and the CSA peak value was reached about 1 week before the peak value for CFU‐C in the bone marrow. Thus, in vivo CSA‐producing cells appeared to be more resistant to CCNU than were CFU‐C, and their recovery appeared to be a prerequisite for the recovery of CFU‐C and m

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1983.tb02129.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

The in vivo and in vitro effects on the aggregatory response of human platelets to ADP and collagen of a series of imidazole and non‐imidazole histamine H2 receptor antagonists, and imidazole derivatives, have been studied. Bolus i.v. administration of the antagonists cimetidine and oxmetidine was without effect. However, inhibition of platelet aggregation was observed in vitro with oxmetidine, metiamide and to a lesser extent burimamide, but not with cimetidine or the non‐imidazole antagonist ranitidine. Of the imidazole derivatives only imidazole and its 1‐methyl analogue significantly affected platelet aggregation. The relationship between potency as a histamine H2 receptor antagonist, the presence of an imidazole ring structure and the antiaggregatory effectiveness of these compounds is discussed. Although certain antagonists clearly inhibit platelet aggregation in vitro, effects are only seen at drug concentrations exceeding those achieved under normal therapeutic conditions; thus the clinical significance of these observations remains to be deter

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1983.tb02130.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

We describe a 10‐year‐old girl with familial haemochromatosis which is associated with a normal concentration of S‐ferritin, myocardial disease and diabetes mellitus. We speculate that iron is accumulated primarily in the heart and pancreas as such isoferritins are not detected by the routine assay of serum ferritin based on antispleen or placenta ferritin. Further, this condition is compared with lysinuric protein intolerance, an inborn error of diamino‐acid transport where an opposite discrepancy between S‐ferritin and storage iro

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1983.tb02131.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Haemopoietic progenitor cells capable of producing granulocyte‐macrophage (GM) colonies have been demonstrated in the ‘null’ lymphocyte population of normal peripheral blood. The helper and suppressor roles of different T cell subpopulations have been implicated in the regulation of granulopoiesis in disease as well as in normal individuals. However, it is not certain whether such interactions between T cells and progenitor cells are HLA‐restricted. We undertook further investigation of the effect of T cell subpopulations (TGand TnonG) on GM colony formation in vitro. In particular, we studied the possibility of HLA restriction in this process. Our results demonstrate that the enhancement of GM colony growth by T lymphocytes is not restricted by HLA compatibility between T cells and null cells, that such stimulation is radio‐sensitive and that it is provided by the TnonGcell subp

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1983.tb02132.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

The effect of hydrocortisone (HC) on the formation of granulocyte‐macrophage (GM) colonies was assessed in vitro in cultures of normal human bone marrow. Subphysiological concentrations (≤ 10−8mol/l) of HC had no impact on GM colony formation, but at physiological and therapeutic levels (10−7mol/l‐10−5mol/l) the hormone stimulated the production of an increased number of colonies. By contrast, physiological concentrations of progesterone (10−9mol/l‐10−7mol/l) were without effect and even at 10−6mol/l there was no influence of progesterone alone on GM colony formation. However, progesterone at 10−6mol/l inhibited the stimulant effect of HC to a degree which was related inversely to the concentration of HC and the duration of the culture interval. The data from this study suggest that the neutrophilia which accompanies glucocorticosteroid therapy is matched by an increase in the production of granulocytes and that HC may play a rôle, which is mediated by steroid receptors, in the physiological regulation o

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1983.tb02133.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

We present the results of the treatment of 15 adult patients with chronic autoimmune thrombocytopenic purpura with high dose i.v. gammaglobulins. In 11 patients, this treatment produced a remission, complete (platelet counts above 100 times 109/1) in 5, partial (platelet counts between 50 and 100 times 109/1) in 6. In all these patients, the response was transient lasting 2–4 weeks. In 4 patients, no response was observed. We used plasmin‐cleaved gammaglobulins which caused rare and mild side effects and gave results similar to those reported with intact human gammaglobulins. The indications of high dose i.v. gamma‐globulins in the management of adult patients with autoimmune thrombocytopenic purpura are disc

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1983.tb02134.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

The excretion of methylmalonic acid was measured and deoxyuridine suppression test (dU‐test) was performed in 5 patients before and after 24 h of ventilation with nitrous oxide. The mean urinary 2‐methylmalonic acid was increased from 90 to 320 μmol/24 h and the dU‐tests became abnormal. These findings indicate that the 2 vitamin B‐12 dependent enzymes, methylmalonyl‐CoA‐mutase and methione synthetase, are depressed by n

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1983.tb02135.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Lactic acid dehydrogenase (LDH) is a glycolytic enzyme that may be elevated in the serum of patients with non‐Hodgkin's lymphoma (NHL). In this investigation LDH was assayed in sera from 155 untreated patients with NHL. Serum LDH (S‐LDH) was found to be significantly correlated both to the spread of the disease and to the histological grade of malignancy i.e. more advanced disease or more aggressive histopathology was associated with higher S‐LDH values. A high pretreatment S‐LDH level (>8.0 μkat/l) in stages III and IV correlated significantly to a decreased survival time. The patients with a pretreatment level of8.0 μkat/l (P8.0 μkat/l) relapsed during or a short time after radiotherapy. In a longitudinal study of 24 patients it was found that S‐LDH reflected in the clinical course. In patients treated to partial or complete remission, S‐LDH decreased and at relapse it rose again. It is concluded that S‐LDH might be useful both as a prognostic marker and to monitor the co

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1983.tb02136.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

The value of a depot cobalamin preparation Betolvex® (cyanocobalamin‐tannin complex suspended in a sesame‐oil aluminium‐monostearate gel) in the maintenance therapy of pernicious anaemia was investigated in 120 patients followed up for between 8 and 15 years. Complete haematological remission was maintained in all patients, and none developed B12neuropathy after the start of treatment. No side‐effects were observed. The mean values of S‐cobalamin were above the reference limits when 1 mg cobalamin was given every 2 months, and high within the reference limits when given at 3 monthly intervals. The cobalamin content in the liver tissue in 2 patients who had received 1 mg cobalamin every 3 months for 6 years revealed normal values. Thus, 1 mg of the depot cobalamin preparation given at intervals of 3 months should provide adequate maintenance therapy in pernicio

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1983.tb02137.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY