摘要:

An ultrastructural study of diffusion chamber granulocytic colonies is reported. The colonies were derived from normal rat bone marrow cells and the chambers were inserted into the peritoneal cavity of lethally irradiated rats.The colonies were processed en bloc for electron microscopy enabling observation of cellular relationship to be made. Ultrastructural analysis of colonies disclosed normal maturing cells at all stages of maturation, with the exception of occasional granulocytes of neutrophil type that presented narrow strips of chromatin (‘nuclear blebs’). The cells in colonies generally occurred in close apposition to one another and to occasional macrophages. This organization possibly enhanced transmission of factors of maturation.Advantages of diffusion chamber haematopoietic cultures are discussed; among the most important are the occurrence of fibroblasts originating from implanted marrow cells and the local formation of fibrin within the chamb

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb01079.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

Two patients with acute myeloblastic leukaemia were found to have a population of bone marrow cells with C‐group monosomy. Using the Quinacrine Mustard fluorescence technique the lacking chromosome was in both patients identified as a number 7. Both patients entered a complete remission. Monosomy 7 may indicate a separate entity of acute myeloblastic leukaemia associated with a comparatively favourable prognosi

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb01080.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

Lymphocyte death in peripheral blood of the mouse represents the death of both short‐ and long‐lived lymphocytes. The present data were based on lymphocyte separation, supravital dye exclusion, and autoradiographical procedures. The data strongly indicate that the frequency of intravascular cell death in the population of short‐lived lymphocytes is 4–10 times higher than the frequency of intravascular cell death of long‐lived ly

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb01081.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

A patient with clinical and haematological findings compatible with congenital dyserythropoietic anaemia (CDA) most probably type II is described. In contradistinction to previous observations, electron microscope examination of the erythroid cells in the bone marrow showed the concomitant appearance of nuclei at different maturation stages in a single cell. In addition, a marked tendency toward multinuclearity of the megakaryocytes was found. The mature erythroblasts showed an increased uptake of3H‐uridine, while the proerythroblasts revealed an uptake of3H‐thymidine less than is normally found in these cells. Thes findings suggest an impairment in RNA and DNA synthe

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb01082.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

A 13 year old girl with the typical clinical and haematological picture of congenital dyserythropoietic anaemia (CDA) is reported. The bone marrow is highly cellular with 70 per cent erythroid cells, and 28% bi‐ and multinuclear cells among the orthochromatic and polychromatic erythroblasts. Moderate megaloblastoid changes are present. On light microscopy the findings are in agreement with those described in CDA type II. On electron microscopy both the cytoplasmatic changes described as typical of CDA type II, and the nuclear changes found in type I can be demonstrated. The acidified serum test (Ham test) is negative with normal sera. The patient's red blood cells show increased agglutinability with anti‐I and anti‐i antibodies, but no haemolysis. Total serum lipids are about 50% of the normal average. All lipoprotein classes are lowered to about the same extent. The total phospholipid content of the erythrocytes is slightly reduced, with a moderate, relative increase of the lecithin fraction and a decrease of the sphingomyelin fraction.Doubts can be raised about the tenability of the current classification of CDA based on morphological and serological criteria, especially about the distinction between types I a

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb01083.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

22 HL‐A antigen and mixed leukocyte culture‐matched sibling bone marrow transplants were attempted in patients with acute leukaemia (at the National Cancer Institute) to define the toxicities of four different immunosuppressive regimens, the complications associated with warrow engraftment and antileukaemic effect. 73% (16/22) were engrafted as indicated by a change to donor red blood cells (RBC) type, leukocyte, immunoglobulin allotype or by the speed of marrow repopulation and the occurrence of the Graft Versus Host Disease (GVHD). 12 of 16 (75%) successful engrafted patients developed GVHD.The current published results of clinical bone marrow transplantation from major centers has been reviewed and will be discussed in relationship to current clinical complications associated with bone marrow transplantat

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb01084.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

Rabbits with Streptococcus viridans aortic valve endocarditis develop anaemia and reticulocytosis which increase with the duration of infection. Mean red cell counts decreased from 6.05 ± 0.29 × 106per μl before infection to 4.10 ± 0.18 × 106per μl after 11 to 20 days of endocarditis and reticulocytes increased from 1.16 ± 0.14 × 105per μl to 4.91 ± 0.83 × 105per μl after more than 20 days of endocarditis. The anaemia could not be explained by intravascular haemolysis. Anti‐erythrocyte antibodies were not detected. Splenomegaly was a consistent finding and also increased with the duration of infection. Red cell half life (T½) was shortened to 4.7 ± 0.3 days in rabbits with endocarditis compared with normal T½ of 11.1 ± 0.5 days. The T½ of red cells from infected animals was prolonged when measured in noninfected rabbits and splenectomized animals had a mean red cell T½ of 9.25 days after three weeks of infection. These studies suggest that splenic enlargement associated with infection results in red cell sequestration, a mechanism not well recognized as contributing to the anae

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb01085.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

Cytogenetic study by a chromosome banding technique has been attempted in 93 cases of acute leukaemia at diagnosis. Banding patterns were difficult to visualise in the bone‐marrow chromosomes of patients with acute leukaemia because of the fuzzy appearance of the fixed metaphases. The proportion of patients with abnormal chromosomes was higher in acute lymphoblastic (ALL) than in acute myeloid (AML) leukaemia. Abnormalities were present in all cases of other cytological types. Hyperdiploidy was the most commonly found numerical error in both ALL and AML but a larger proportion of patients with ALL had hyperdiploidy in more than 30% of the cells. In ALL it was generally found that the higher the frequency of hyperdiploidy the greater was the number of chromosomes per cell. Hypodiploidy not attributable to random losses was found in only 6 patients. Clones identified by rearranged or marker chromosomes were found in all types of leukaemia. Clones marked by a 7q‐chromosome, in which the break point was the same, were identified in 1 adult with ALL and 2 children with AML. The high frequency of randomly distributed chromosomal breakage found in the bone‐marrow chromosomes of a high proportion of the patients may be related to the disease pr

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb01086.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY