摘要:

A quantitative evaluation of erythropoiesis was carried out in 12 patients with hairy cell leukaemia (HCL). The results were compared with those obtained in eight patients with aplastic anaemia (AA) in order to define the characteristics of erythroid failure in HCL. Discriminant analysis was applied to both haematological and erythrokinetic parameters of the two disease groups. Plasma iron concentration and MCV were significantly higher in AA, and allowed a perfect separation of the patients. As regards erythrokinetics, values of ineffective erythropoiesis and peripheral haemolysis were able to separate completely the two disease groups, being significantly lower in HCL than in AA. A true erythroid failure was the peculiar erythrokinetic pattern of HCL. This conclusion allows one to speculate on the nature of the stem cell damage in this disease.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1982.tb00501.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

The karyotypic pattern in 69 patients with Ph1‐positive chronic myeloid leukaemia (CML) was investigated during the blastic phase (BC) and correlated with survival and certain parameters of potential prognostic significance, including blast morphology, basophilia and thrombocytopenia.There was no difference in median survival in BC between patients with and without aberrations in addition to the Ph1. Nor were there any differences in this respect among patients with the specific aberrations +Ph1, +8, iso(17q), or other abnormalities. There was no correlation between the incidence of thrombocytopenia and any particular karyotypic change. However, the incidence of basophilia was a characteristic feature for patients with an iso(17q).The survival time in BC was considerably longer in patients with a lymphoid morphology of the blastic cells compared to the myeloid varieties, and within the myeloid varieties the survival in BC was longer in patients with granular differentiated blasts than in those with granular atypical blast cells.No obvious correlation was apparent between blast morphology and karyotypic pattern. However, a pattern was discernible regarding survival and certain chromosomal changes within some morphologic groups: in patients with granular differentiated and lymphoid morphology, the median survival in BC was considerably longer when the bone marrow cells had a Ph1as the sole abnormality compared to patients who had additional aberration

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1982.tb00502.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

Ivy bleeding time, capillary fragility, threshold ADP concentration for secondary platelet aggregation and platelet adhesiveness were found to be unchanged by 2 d and 6 weeks of treatment with prednisone in 22 consecutive patients with collagen or haematological diseases. Initial high platelet counts were unchanged after 2 d of treatment, but fell significantly to normal values after 6 weeks of treatment. Initial high levels of factor Vlll‐related antigen increased insignificantly following 2 d of treatment, but after 6 weeks of treatment the increase was significant. It is concluded that in patients of this category a 6‐week treatment with commonly used doses of prednisone does not significantly affect platelet funct

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1982.tb00503.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

The effects of serum from patients with chronic lymphocytic leukaemia (CCL) on normal polymorphonuclear leucocyte migration (PMN) were examined by means of the leading front technique, using a modified Boyden chamber. 18 randomly selected patients were studied. 13 patients had a reduced chemokinetic activity. The defective migration was explained by the finding in serum from these patients of cell‐directed inhibitory activity which was destroyed by heating (56°C, 30 min). The B‐lymphocytes as the origin of the inhibitory activity was suggested by the presence of a similar activity in supernatants from cultured tumour cells. 6 of the 18 patients had the combination of a defective chemokinetic activity and low levels of immunoglobulins. These 6 patients had an increased tendency towards infect

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1982.tb00504.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

A 73‐year‐old previously healthy woman was admitted because of severe bleeding from esophagitic lesions and intraabdominal bleeding following hysterectomy. Acquired hemophilia, probably due to an IgG antibody to factor VIII (64 inhibitor units/ml) was noticed, the VIII:C in the patient's plasma being 18% of normal.Immune complexes isolated by polyethylene glycol precipitation had only a weak factor VIII inhibiting activity whereas IgG purified from the complexes and monomeric IgG present in her plasma exerted a strong inhibition. Removal of the complexes from plasma had no effect on the inhibitor titer thus indicating that only a minor part of the antibody was circulating as immune complexes.Plasma or purified IgG from the patient decreased the VIII:C of normal plasma to 18 og 14%, respectively, total inhibition being impossible ot achieve even in antibody excess, probably reflecting residual activity of factor VIII bound to the patient's antibodies. The ristocetin cofactor activity of normal plasma was unaffected by the antibodies. Transfusion of factor VIII concentrate to the patient resulted in therapeutic levels of circulating factor VIII and transfused factor VIII circulated longer than usual. Partial remission of the disease with adequate levels of VIII:C occurred after 3 mon

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1982.tb00505.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

The diurnal variations in cobalamin‐binding plasma proteins were studied in 10 healthy volunteers. The unsaturated cobalamin‐binding capacities of transcobalamins (TC‐II and R‐binders), P‐cobalamin and P‐protein were measured every third hour during 24 h. Only small variations within an interval of ± 20% were found. Highly significant decrements during the night were a uniform finding. After correction for variations of total plasma protein, the changes became less prominent, but still significant for P‐cobalamin and P‐R‐binders. Hence the mean values of P‐R‐binders and P‐cobalamin were 113 and 533 pmol/1 at 9:00 h, and decreased 12 and 8%, respectively, at 3:00 h.When using cobalamin binding plasma proteins in the monitoring of various malignant, haematological and autoimmune disorders, the specimens should, therefore, be collected at defined hours

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1982.tb00506.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

The detection of a γ‐heavy chain disease protein in the serum and urine of a patient with a history of Hodgkin's disease in complete remission is reported. The protein has been characterized as a γ1dimer, MW 66000, beginning at Asp 221 residue in the hinge region. No clinical or laboratory findings of malignancy have been observed in the patient since the abnormal protein was detected. The pathogenesis of this association, unreported to date, is discussed on the basis of previously administered high‐dose anti‐neoplastic treatments and of immunological imp

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1982.tb00507.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

In order to describe the changes of the human bone marrow compartments after repeated courses of adriamycin/cyclophosphamide in quantitative terms, 2 methods have been studied for their usefulness as indices of bone marrow cellularity: cell counts per ml of bone marrow aspirates obtained under standardized conditions, and cell counts per μl of bone marrow spicules evaluated by morphometric techniques.Applied to a sufficiently large group of patients, both methods were useful to describe the cytotoxic drug‐induced changes of the bone marrow compartments in accordance with the following indirect criteria: (a) the results in man are quite compatible with the changes of haematopoiesis described in animal experiments after cytotoxic drugs; (b) the changes of the peripheral blood cell pools ‐ PMN and reticulocytes ‐, which can be assessed quantitatively, correspond to the changes of the respective bone marow pools as determined by the described indices of cellularity; (c) this quantitative description of drug‐induced changes in the human bone marrow compartments yields ‘reasonable’ results on the basis of both the mechanism of action of the cytotoxic drugs employed and the kinetics of the haematopoietic sy

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1982.tb00508.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

Peripheral blood lymphocytes from 22 consecutive patients with chronic lymphocytic leukaemia were stimulated with the polyclonal B‐cell mitogens lipopolysaccharide from E. coli (LPS) and Epstein‐Barr virus (EBV). Stimulation was successful for chromosome analysis in 14 patients. Eleven patients had chromosomal aberrations and 7 of these had an extra chromosome 12. In 2 patients an extra chromosome 12 was the only abnormality, while additional aberrations were found in 5 patients. 3 patients had complex aberrations involving deletion of chromosome 6. 1 of these patients also had a translocation between chromosomes 12 and 14. 1 patient had a translocation between chromosomes 11 and 14. In 3 patients no aberrations were detected. The time elapsing between diagnosis and appearance of clinical symptoms which were indications for treatment was significantly shorter in patients with an extra chromosome 12 than in those without this abnormality. Thus, it appears that an extra chromosome 12 is associated with a more rapid course of the disease, and may therefore be of importance for the prediction of progno

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1982.tb00509.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

A patient with thalassaemia intermedia and haemosiderosis is reported. This patient did not receive transfusions or iron therapy. The iron absorption and the plasma iron turnover (PIT) were increased. Transfusions were carried out in order to decrease the amount of abnormal erythropoiesis. After that, the erythropoietin and PIT were decreased to normal levels and the iron absorption also returned to normal. The data presented suggest that increased erythropoiesis was responsible for the abnormaly high iron absorption and subsequently for the haemosiderosis of the patient presented.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1982.tb00510.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY