ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1980.tb01311.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Bacterial infections and trauma which increase production of granulocytes and monocytes by the bone marrow, may do so through factors in serum capable of stimulating growth of granulocyte‐macrophage cells in vitro. Human serum possesses two types of colony stimulating activity (CSA), one which stimulates granulopoietic progenitor cells directly, and another which results from the interaction of serum and bone marrow adherent cells (monocyte‐macrophages) or peripheral blood leucocytes:adherent cell dependent CSA. These activities are due to different factors which may be separated by gel filtration.Sera of 7 patients undergoing hystectomy who developed post‐operative infection showed post‐operative elevation of the adherent cell dependent activity in all cases but no change in direct acting CSA. These results suggest that the direct acting CSA in human serum does not represent the principal humoral ‘message’ to the bone marrow from sites of trauma and infection in the tissues and that granulopoiesis may be controlled indirectly by the action of a different humoral factor which increases production of CSA by marrow monocyte‐macrophages. Preliminary experiments suggest that lymphocytes stimulated by bacterial products may be one source o

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1980.tb01312.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Continuous‐flow leukapheresis using the AMINCO Celltrifuge® was performed in 35 adult volunteers for the procurement of large quantities of granulocyte/macrophage progenitor cells (CFUc) as part of the mononuclear leucocytes.The donor blood volume processed was 12 1 at a flow rate of 50 ml per min. The centrifuge speed was 800 rpm. The mean yield of mononuclear cells (MNC) per run was 12.0 times 109and that of CFUc, 8.7 times 105. The leukapheresis did not much affect the donor blood cell concentration except the platelet concentration; this must be considered as a limiting factor for leukapheresis. The mean granulocyte contamination of the leukapheresis‐derived leucocytes was only about 10%.Successive leukaphereses (with short‐term intervals) in 7 donors led to significant increases in CFUc yield.The possible therapeutic application of blood stem cells in the treatment of haemopoietic failure in man is dis

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1980.tb01313.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

An analysis of prognostic factors was performed on a series of 92 adult patients with acute non‐myeloid leukaemia (ANML). Four factors were shown to be correlated with a poor short‐term prognosis: (1) Neuropathy involving a single cranial nerve (numbness of the chin) as a presenting feature. (2) A low percentage blast cell infiltration of bone marrow (>55%). (3) Cytological L2type or L3type of cells according to FAB classification proposals. (4) Increased age.Two factors were shown to be correlated with a poor long‐term prognosis: 1) A high level of circulating blast cells; 2) A high tumour burden, as in childhood ANML. These two long‐term prognostic factors were interrelated. In addition, our results suggested that myelofibrosis could be a poor long‐term prognosis facto

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1980.tb01314.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

2 translocations, t(11;14) and t(1;6), were found in 24 out of 46 metaphases in bone marrow cells from an untreated patient with plasma cell leukaemia. The predominating cell population produced only kappa chains while a minute population produced IgG kappa. All serum Ig's were low and only minimal amounts of monoclonal IgG kappa were found in the serum and very small amounts of kappa light chains in the urine. Analyses of ours and 2 other reported patients indicated the possibility that the location of breakpoints on the chromosomes may be of etiologic importance for the type of light chain and perhaps for the class of heavy chain.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1980.tb01315.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

A case of severe but transient haemolytic disease occurring after a febrile episode is described. The thermal amplitude of the haemolysin was high during the acute phase, since the autoantibody fixed complement at 31° C. After 4 months complement fixation could exclusively be demonstrated at 4° C. The patient was treated in a room heated to 30–32° C. The treatment consisted of prednisone and azathioprine and during the acute phase plasmapheresis was attempted in order to reduce the antibody concentration. However, the haemolysis decreased when the thermal amplitude of the antibody diminished. 1 year after termination of therapy, she developed sarcoid

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1980.tb01316.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

The marrow myeloid precursor cells of a haematologically normal patient were labelled by intravenous injection of tritiated thymidine. Young labelled segmented neutrophils were then released from the marrow into the blood by an injection of Cortisol. These PMN had a significantly lower LAP activity than the older blood neutrophils. It is shown that within the morphologic boundaries of the segmented PMN, the cells are still in different stages of cytoplasmic maturation. In addition, labelled and unlabelled neutrophils showed a linear and parallel increase of LAP activity during the 24 h following Cortisol injection. This observation suggests that the neutrophil prematurely released in the blood can mature into normal LAP‐PMN and more generally that LAP activity of a blood neutrophil increases with tim

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1980.tb01317.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Young red cells obtained by high‐speed centrifugation of a normal blood sample had an MCHC of 31.7 g/dl which corresponds to an internal viscocity of about 9 cP. Old red cells from the same blood sample had an average MCHC of 37.5 g/dl which is equivalent to a mean internal viscosity of about 54 cP. This increased internal viscocity of old cells must result in a prolonged transit time through the narrow channels of the splenic microvasculature and consequently an increased probability of contact with a phagocytic macrophage. This probability of contact increases as the internal viscosity of the cell is further increased and may represent one of the major factors associated with the detection and subsequent elimination of senescent cells by the splee

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1980.tb01318.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Serum selenium levels were measured in 38 patients with malignant lymphoproliferative diseases (MLD) and in 34 non‐hospitalized healthy individuals. Selenium was determined by proton induced x‐ray emission.In Hodgkin's disease and non‐Hodgkin malignant lymphoma the mean serum levels of selenium were not different from those of the control group. On the contrary lowered mean serum selenium concentrations were observed in the group with chronic lymphocytic leukaemia (5.2 + 0.7 μg/100 ml) as compared to normal individuals (7.9 + 0.3 μg/100 ml). The difference is highly significant (P<0.005).A second selenium test was made in 11 out of the 38 patients within 8 weeks from the beginning of radiotherapy or chemotherapy; unchanged levels wer

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1980.tb01319.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

In 19 jaundiced newborns who had no evidence of kernicterus the effect of bilirubin on adenosine‐5′‐diphosphate (ADP), epinephrine and collagen‐induced platelet aggregation was studied. Compared to 20 normal adult controls, no significant difference was found for ADP and collagen‐induced platelet aggregation in jaundiced infants. However, with epinephrine, significantly decreased aggregation was observed, but this was also the case for non‐jaundiced infants. This suggests that the reduced response to epinephrine in hyperbilirubinaemic infants is related to age rather than to bilirubin. Increased bilirubin is therefore not responsible for any increased bleeding tendency due to impaired platel

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1980.tb01320.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY