摘要:

The regulatory influence of autologous blood monocytes on the PWM‐induced generation of Ig‐secreting cells was assessed using a reverse haemolytic plaque forming cell (PFC) assay. The PWM‐induced PFC responses of monocyte‐depleted cells were low or absent in most cases. Addition of 12–42% freshly isolated monocytes fully reconstituted the IgM‐, IgG‐ and IgA‐PFC responses. With more monocytes added, the PFC responses declined. Monocytes precultured for 48 h supported the PFC responses of monocyte‐depleted cells less well and addition of monocytes stimulated with phorbol myristate acetate (PMA) did not increase the response. The low responses of monocyte‐depleted cells co‐cultured with precultured monocytes were not increased by addition of supernatant from monocyte cultures.The PFC responses of mononuclear cells induced by PWM were significantly inhibited by unstimulated precultured monocytes, and to a larger degree by PMA‐treated monocytes, indicating the presence of suppressor cells among the precultured monocytes. The PWM‐induced thymidine incorporation by monocyte‐depleted cells with precultured monocytes added was only slightly lower than that obtained with freshly isolated monocytes added, suggesting that the suppressive role of precultured monocytes

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1982.tb00594.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

2 patients with early‐stage chronic lymphocytic leukaemia had involvement of the central nervous system causing dementia as the predominant clinical symptom. None of the patients had focal neurological abnormalities. One patient was successfully treated first with irradiation therapy to the central nervous system, and later with intrathecal administration of methotrexate. The other patient died with massive leukaemic infiltration of the central nervous system before effective treatment had been given. Although significant involvement of the central nervous system is rare in chronic lymphocytic leukaemia and usually does not occur until late in the disease, these cases show that clinically important infiltration may happen very early. The clinical picture of dementia has not been described in chronic lymphocytic leukaemi

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1982.tb00595.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

A new autosomally inherited dysfibrinogenaemia was recognized in 3 members of an Italian family. No bleeding tendency or thrombotic disease in any of the affected members were demonstrated.Coagulation tests revealed prolonged prothrombin, thrombin and Reptilase times. Plasma fibrinogen levels were normal with immunologic method and slightly reduced with chrono‐metric assay: the other blood coagulation factors were normal. In addition, cross‐immuno‐electrophoresis performed on patients' plasma was indistinguishable from the normal.Dysfibrinogenaemia was confirmed by studying the purified fibrinogen. The fibrin polymerization curve, measured spectrophotometrically, showed a lower slope than the normal. A delay in fibrin monomer aggregation was revealed when compared to the normal at an equal concentration. The release of fibrinopeptides was normal. SDS polyacrylamide gel electrophoresis, isoelectric focusing and cross‐immunoelectrophoresis of purified fibrinogen were not able to demonstrate any structural abnormality. The fibrinogen was named fibrinogen

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1982.tb00596.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

A mixed B‐cell type centroblastic lymphoma with extraordinarily many histiocytes in a 68‐year‐old man is reported. Multiple skin tumours were the only clinical manifestation during the first 5 months. The disease then progressed to the lymph nodes, spleen, and parenchymal organs, but the bone marrow remained unaffected until death, 11 months after the onset of signs. C3d receptors were the only surface markers of the centroblasts. The histiocytes were normal with respect to morphology, muramidase staining, and Fc and C3b receptors. This highly unusual spread from skin to lymphoid and parenchymal organs is discussed in the light of lymphoid cell kin

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1982.tb00597.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

A 16‐year‐old girl with severe aplastic anaemia was successfully treated with retransplantation of bone marrow from an HLA‐identical sibling after rejection of the first transplantation from the same donor. Cyclophosphamide was used for the first transplantation and cyclophosphamide, 300 rad total‐body irradiation and antilymphocyte globulin were used for the second transplantation. Permanent engraftment was achieved after the retransplantation with normalization of haemopoiesis, which has lasted for over 17 months. The patient is now in excellent clinical condition with minimal signs of chronic graft versus host

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1982.tb00598.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

The prevalence and causes of anaemia among the inhabitants of a Southern Lebanese village were studied. Of the 317 patients examined 75, or 24% were found to be heterozygotes for Hb S. There was a reciprocal relation between the presence of sickle cell trait and of iron deficiency anaemia. The prevalence of iron deficiency anaemia defined as the coexistence of Hb below 13 g/dl in adult males and below 12 in adult females and children with two laboratory indicators of iron deficiency, was 10.3 % in the normal population as against 1.3 % in the sickle cell trait group (P<0.008). The reduced frequency of iron deficiency anaemia in sickle cell trait may be explained by increased iron absorption, or alternatively by reduced iron requirements and a lower risk of discrepancy between iron supply and demand. Because of the central role of iron in the function of a great number of enzymes and proteins, it is possible that protection against iron deficiency anaemia by the sickle cell trait may result in improved working capacity and a lower incidence of infections.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1982.tb00599.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

Thin‐layer chromatography and bioautography were used to study the cobalamin pattern of plasma, erythrocytes, and hepatic tissue from patients with cobalamin deficiency on maintenance therapy with hydroxocobalamin (Vibeden®), a cyanocobalamin depot preparation (Betolvex®), or cyanocobalamin tablets (Behepan®). The cobalamin pattern in plasma is dominated by the form in which it is administered. The results of assaying the erythrocytes and liver biopsies show that the cobalamins administered are converted to the coenzyme forms in vivo, irrespective of the type of cobalamin prepara

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1982.tb00600.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

Growth patterns of marrow and blood erythroid progenitors were studied in 18 cases of pure erythrocytosis using different doses of erythropoietin. 8 cases demonstrated ‘spontaneous’ growth of CFU‐E and blood BFU‐E as observed in myeloproliferative disorders, but without an excess of circulating CFU‐GM. 3 of these patients also had other symptoms of a pan‐myelopathy. All these cases showed good sensitivity to32P myelo‐suppression. 10 cases demonstrated growth patterns of erythroid progenitors similar to those observed in normal subjects, except for an excess of blood BFU‐E, which suggests an abnormality of homeostatic regulation. In 5 of these cases, myelo‐suppression was not effective.It is suggested that a stem cell study could differentiate patients with pure erythrocytosis due to ‘autonomous’ abnormal stem cell growth from cases due to abnormal regulation factors, and that such a discrimination might be useful for

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1982.tb00601.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

We studied circulating erythroid and granulocyte‐monocyte progenitors in 18 patients with idiopathic myelofibrosis and in healthy controls, using the methyl cellulose assay. 9 of the patients had been splenectomized prior to the study. The median number of circulating erythroid burst‐forming units (BFU‐E) was 8 times higher than that of the controls. 12 patients also had CFU‐E (colony‐forming unit, erythroid) in the blood. 10 patients had spontaneous BFU‐E colony formation, and 8 patients had spontaneous CFU‐E colony growth. Granulocyte‐monocyte progenitors (CFU‐GM) were increased 47 times compared to the controls. There were no differences in colony numbers between splenectomized and non‐splenectomized patients. We conclude that in myelofibrosis, circulating erythroid and granulocyte‐monocyte progenitors are usually markedly increased in number, but erythroid precursors to a lesser extent than granulocyte‐monocyte precursors. Many patients, but not all, show spontaneous ery

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1982.tb00602.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

Aprindine, a potent anti‐arrhythmic agent, occasionally seems responsible for agranulocytosis. In order to study its potential haematological toxicity, 3 different in vitro tests were used: (a) the capacity of human and mice bone marrow to incorporate tritiated thymidine (3HTdR), (b) the capacity of stimulated human blood lymphocytes to incorporate3HTdR and (c) the capacity of human granulocyte‐macrophage stem cells to form colonies in agar. For all these tests aprindine was found to be toxic at concentrations close to the clinical therapeutic serum concentration.Moxaprindine, chemically very close to aprindine exhibits also an anti‐arrhythmic activity. It was examined in the same tests in parallel with the study af aprindine. Moxaprindine also exhibited haematological toxicity in the tests but at a significantly higher concentration, approximately twice that of aprindine. Assuming that these in vitro tests are relevant to the in vivo haematological toxicity, moxaprindine could be considered a clinically safer antiarrhythmic agent than apri

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1982.tb00603.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY