摘要:

The endogenous production of14CO during the first week after administration of glycine‐2‐14C (early labelled peak, ELP) was determined in 13 healthy subjects. The subjects were studied during normal, suppressed or stimulated erythropoiesis induced by hypertransfusion and phlebotomy, respectively. The production of14CO was calculated from determinations of the specific activity of the body CO store and the endogenous production of CO. The incorporation of labelled glycine into circulating red cell haemoglobin haem was calculated from the maximal specific activity of haemoglobin haem and the total amount of Hb. The average ELP was 73.0 × 103disintegrations per min (dpm) and the incorporation of glycine into circulating haemoglobin haem 491 × 103dpm in controls. The ELP and the incorporation of glycine increased by 86% and 92%, respectively, in phlebotomized subjects and decreased by 37% and 62%, respectively, in hypertransfused subjects. A linear regression equation was calculated for the relationship between ELP and erythropoiesis, expressed as the incorporation of glycine into circulating haemoglobin haem. The residual ELP remaining in the absence of erythropoiesis was 24.9 × 103dpm calculated from data of all the subjects, and 32.8 × 103dpm calculated from data of controls and hypertransfused subjects only. In conclusion, erythropoietic haem turnover was found to contribute about 60% and nonerythropoietic (mainly hepatic) haem turnover about 40% of the ELP

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1980.tb01585.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

The determination of the early peak of14CO production that results from haem turnover after administration of glycine‐2‐14C was studied in patients with pernicious anaemia. The accuracy of this technique in measuring ineffective erythropoiesis was assessed by comparison with other measurements of the severity of the disease. 6 patients were studied before specific treatment and 2 of these also after treatment. The endogenous production of14CO was calculated from determinations of the production of CO by a rebreathing technique and of the specific activity of CO washed out from the body CO store by oxygen breathing. The early labelled peak was increased 7–39 times compared to that in healthy control subjects and represented 35–86% of the total production of14CO as compared to 13% in controls. The early labelled peak was significantly correlated to the Hb concentration, mean red cell volume and endogenous production of CO. 2 patients, twice examined, showed a moderately increased early labelled peak also after specific treatment. This was attributed to persistence of some ineffective erythropoiesis in 1 patient and possibly increased hepatic haem turnover in the other. The magnitude of the early labelled peak and the maximal activity of14CO found in the samples of CO collected from the body CO store were significantly correlated. In conclusion, with the present method the early peak of14CO reflected the ineffective erythropoiesis in patients with pernicious anaemia. The early labelled peak could be predicted from the maximal activity of14CO found in the samples of CO washed out from the body C

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1980.tb01586.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

26 children with acute lymphoblastic leukaemia (ALL) and 7 additional patients with lymphosarcoma in leukaemic transformation (LSA) have been studied with respect to the content of myelopoietic stem cell (CFUc) in blood and bone marrow. The methylcellulose culture technique (Iscove et al 1974) was employed in the absence of an exogenous source of colony stimulating factor (CSF). During active disease, CFUc colony formation was absent from patients with ALL, but was present in 2 patients with LSA. 2 therapeutic regimens were employed. Colony formation from bone marrow CFUc was highly variable during remission maintained by either regimen, with no clear relation to clinical stage, number of monocytes or circulating neutrophils. Patients with LSA consistently had high numbers of bone marrow CFUc. CFUc were low or absent from the blood. In conclusion, CFUc are absent from the bone marrow in active ALL, but may be present in active LSA. For the purpose of monitoring children with ALL during therapy, determination of blood or bone marrow CFUc was not found in this study to be helpful.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1980.tb01587.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Surviving macroreticulocytes are released to peripheral blood during the first 4 d following a B12injection to vitamin B12deficient patients with grave anaemia. Marked increase in number of reticulocytes, together with corresponding reduction in reticulocyte size is noted in the top layer of the centrifuged packed red column from d 4 to 6 after medication. This ‘remodelling’ of the ‘stress‐reticulocytes’ seems best explained by a division of the cells into 2 equal, surviving, normal‐sized reticulocytes. The macroreticulocytes, which probably result from skipped division in the marrow, may thus complete their last cytoplasmatic division in the peripheral blood. The numerous schistocytes, which are found in the top layer of the centrifuged packed red cell column, disappear during the first few days after treatment, and it is assumed that these cells are fragments from the relatively few megalocytes which have reached the peripheral blood prior to medication.Macroreticulacytes seem to have a maximum deformability on d 5 after the B12injection, shortly after the cell division

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1980.tb01588.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Erythrocyte glucose‐6‐phosphate dehydrogenase (G‐6‐PD) activity was measured in 16 non‐dialysed patients with chronic uraemia, 17 patients on regular peritoneal dialysis, 18 patients on regular haemodialysis, 10 renal transplanted patients with normal renal function, and in 41 healthy control subjects. In non‐dialysed uraemic patients G‐6‐PD values were not significantly different from those in the control group. Peritoneal dialysed patients had slightly, but significantly higher G‐6‐PD values than controls (P<0.01). Haemodialysed patients demonstrated the highest G‐6‐PD values of all groups, being significantly higher than in both controls (P<0.001), non‐dialysed (P<0.01), peritoneal dialysed (P<0.01), and renal transplanted patients (P<0.01). Renal transplanted patients had G‐6‐PD values which not differed significantly from controls. In all uraemic patients G‐6‐PD activity was positively correlated to serum creatinine (rs= 0.64, P<0.001) and negatively correlated to haemoglobin (rs= ‐0.61, P<0.001). In the peritoneal and haemodialysed groups Gd‐PD activity was correlated to the reticulocyte counts (rs= 0.54, P<0.001). The results indicate that a younger mean red cell age is responsible for the increased G‐6‐PD activity in perit

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1980.tb01589.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

In 57 cases of B‐type chronic lymphocytic leukaemia (B‐CLL), the size of peripheral blood lymphocytes was estimated by means of transmission electron microscopy. The mean lymphocyte diameter (MLD) of 50 cells was correlated with the clinical staging as well as the survival. 30 out of 38 patients found in stages 0, I, and II displayed a normal or increased MLD. Conversely, this value was decreased in 12 out of 17 cases in stages III and IV. MLD of patients in clinical stages III and IV was significantly lower as compared with MLD of patients in stages 0, I, and II (P<0.001). The actuarial curve of 57 patients showed a roughly estimated median survival probability of 43 months. This was of more than 54 months in patients with normal or increased MLD, but only of 22 months in those with a decreased MLD. The difference between these 2 survival curves was statistically significant (P<0.01). A reduced peripheral blood lymphocyte size, as estimated in suspension by means of transmission electron microscopy, appears to have a bad prognostic significa

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1980.tb01590.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

During the last 8 years (1971–1979) all newly diagnosed previously untreated patients with Hodgkin's disease in Denmark have been centralized to uniform staging procedures and treatment. A total of 802 patients were registered, or 2 patients /100 000. Lymphangiography was performed in 708 patients (88%), and 437 patients (55%) underwent laparotomy with splenectomy. Treatment included radiotherapy, combination chemotherapy (MOPP or similar programmes), and combined modality treatment. The overall 8‐year actuarial survival for all stages combined was 66%, and relapse‐free survival was 55%. 144 patients died of Hodgkin's disease, 23 from complications to therapy and examination procedures, and 54 died of unrelated causes. Survival was significantly better for patients without B‐symptoms, and decreased gradually with advancing age. There was a strong correlation between unfavourable prognosis and advancing stage and/or histology, but mediastinal involvement had no influence upon the prognosis. Staging laparotomy was associated with 4 deaths due to infection, and splenectomy with 10 cases of severe pneumococcal infections, 4 of which were fatal. Fatal complications due to subsequent treatment included 2 cases of cardiac arrest following mantle‐field irradiation and 3 cases of haemorrhage or sepsis following Chemotherapy. 5 cases of acute myeloid leukaemia were

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1980.tb01591.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Bone marrow smears from 72 consecutive children with a recent diagnosis of acute lymphoblastic leukaemia (ALL) have been morphologically analyzed. A high incidence of lymphoid cells with amoeboid movement configuration (AMC) seems to indicate a more favorable prognosis independently of other prognostic factors e.g. age, WBC or sex. A positive correlation between the AMC and the mitotic indices (MI) of the lymphoblasts indicates some connection between the motility and the proliferative activity of the leukaemic cell clone.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1980.tb01592.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

The in vitro properties of 5 factor VIII preparations (AHF‐Kabi, Hemofil Hyland, AHF‐Profilate Abbott, Kryobulin Immuno and Factorate High Purity Armour) and an ordinary cryoprecipitate were studied with reference to factor VIII clotting activity (VIII:C), factor VIII clotting antigen (VIILCAg), factor VIII related antigen (VIIIR:Ag) (EI, IRMA, CIE), ristocetin cofactor activity (VIIIR:RCF), fibrinogen and factor XIII. All the preparations with the exception of Factorate had higher levels of VIII:CAg than VIII:C indicating inactivation of the biological activity of VIII:C during the procedure. AHF‐Kabi (fraction I‐0) and the cryoprecipitate, the only preparations capable of normalising the defect in patients with von Willebrand's disease, showed the same level of VIIIR:Ag determined by EI and by IRMA, while all the other preparations (i.e. cryoprecipitates purified further in different ways) had considerably lower levels of VIIIR:Ag determined by IRMA than by EL Based on these in vitro techniques it seems to be possible to predict which preparations can be used successfully in patients with von Willebrand's disease, while no such conclusions can be made from VIIIR:RCF determinations.EI yielded similar concentrations of factor XIIIasubunit in all the preparations tested. 3 functional assays showed high factor XIII activities in AHF‐Kabi but low or no activities in the others.Thus, considerable differences were found of the in vitro properties of the proteins in 5 factor VIII concentrates and a cryoprecipitate. The action of proteases and the techniques used in the purification procedure are probably of crucial importance for the properties of the variou

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1980.tb01593.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1980.tb01594.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY