摘要:

A total of 17 patients with hypertension undergoing renal vein or adrenal vein catheterization were investigated in order to ascertain the number of megakaryocytes in blood from the inferior vena cava, the femoral artery and a cubital vein. On an average 11.9, 3.8, and 4.5 megakaryocytes per ml were found, respectively. In blood from the inferior vena cava, 30 % of the megakaryocytes had copious cytoplasm, while megakaryocytes in arterial and cubital venous blood had sparse or no visible cytoplasm. It was demonstrated that ⅔ of the megakaryocytes were retained in the pulmonary circulation and that at least 70% of the platelets could derive from megakaryocytes in central venous blood or the pulmonary circulation. It was found that megakaryocytes pass through a life cycle in which the differentiation take place in the bone marrow, platelet release occurs mainly in central venous blood and in the pulmonary circulation and the destruction of the megakaryocyte nucleus take place outside the bone marrow, especially in the pulmonary circulatio

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1978.tb00381.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Acute myelogenous leukaemia was diagnosed within a 1 week's interval in a married couple without consanguinity. The husband had acute myelomonocytic leukaemia whereas the wife had acute myelogenous leukaemia of the classical type. Neither the histories nor findings contributed to an understanding of leukaemogenesis. The calculated risk that a husband and wife should develop acute myelogenous leukaemia within the same year is approximately 1:400 millions.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1978.tb00382.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Chronic lymphocytic leukaemia (CLL) has a variable clinical course and there is a great need of new prognostic laboratory parameters in this disease. 24 CLL patients were subjected to routine haematological and clinical investigation. The leukaemic cells were analyzed for surface immunoglobulins, complement and sheep red blood cell receptors, Concanavalin A‐induced agglutination, cytoplasmic glucocorticoid receptor and for proliferative activity by measurement of tritiated thymidine incorporation.The surface markers studied indicated that all cases were of B‐cell origin. Only 5 of 23 patients studied had normal serum immunoglobulin levels. These cases showed a nonprogressive disease. 8 patients had increased infection tendency, all of whom had subnormal IgG levels; 4 of them also had subnormal IgA and IgM and 2 had subnormal IgA levels. 5 out of 6 patients with progressive disease and 3 of 11 with nonprogressive disease had an increased proliferative index, indicating a correlation between this parameter and disease progression. ConA agglutinability was not correlated to disease activity. Cells from 17 of 22 patients showed measurable amounts of glucocorticoid receptor. The 5 patients lacking this receptor had inactive dise

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1978.tb00383.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

The effect of treatment with prednisolone on clinical and laboratory variables was studied in 6 patients with chronic lymphocytic leukaemia (CLL). The laboratory variables analyzed on the leukaemic cells were surface immunoglobulin (S‐Ig), sheep red blood cell receptors (SRBC), proliferative activity (PI) and glucocorticoid receptor (GR).In all cases the CLL was of B‐cell type and the leukaemic cells contained a significant amount of GR. 4 out of 6 patients had a progressive disease with increased PI. On treatment they went into clinical remission, which was paralleled by a reduction in the leukaemic B‐cell number and in PI. The amount of GR was unaffected. In 2 patients with nonprogressive disease, prednisolone produced no clearcut effect on clinical or laboratory vari

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1978.tb00384.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

The prognostic significance of the bone marrow sideroblasts found in aregenerative anaemia with hypercellular bone marrow was evaluated particularly to see if the sideroblasts might be a preleukaemic sign.The study includes 32 patients with abnormal sideroblasts; 14 had mainly ringed sideroblasts while 18 had mainly the intermediate or ferritin type. Basic erythro‐kinetic data (59Fe erythrocyte incorporation and erythroblast labelling indices) were similar in the two groups. The colony forming capacity (CFUC) was abnormal in 2 of 5 patients with ringed sideroblasts compared to 12 of 13 in the intermediate sideroblast group. Patients with mainly ringed sideroblasts had significantly longer duration of disease state, higher platelet and leucocyte counts, no increase in the percentage of bone marrow myeloblasts and did less often develop acute leukaemia. Thus aregenerative anaemias can be divided according to the type of abnormal sideroblasts in two groups with different risk of development of acute leukaemia. The ringed sideroblastic anaemia seems not to be a preleukaemic stage in the prospective sense in contrast to aregenerative anaemia with mainly intermediate sideroblast

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1978.tb00385.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

The effect of endotoxin on the processing of erythrocyte iron by reticuloendothelial cells of the liver and spleen was studied in rats using heat damaged erythrocytes labelled with59Fe.Endotoxin did not alter the uptake of the damaged cells but markedly inhibited the subsequent early phase of iron release from the reticuloendothelial cells. The inhibition seemed to be due to both a decreased rate of labelled haem destruction and an increased incorporation of radioiron into ferritin. Although early iron release was decreased 0–2 h after endotoxin administration, the diversion of iron into ferritin was more marked when endotoxin was given 18 h before. The block in iron release was partially overcome in animals that had been kept on an iron free diet or had been phlebotomised. In these animals the decreased rate of haem catabolism remained unaltered but less iron was diverted into ferriti

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1978.tb00386.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Human mature granulocytes added to in vivo diffusion chamber (DC) cultures of murine bone marrow cells (BMC) led to a late reduction of CFU‐C, day 5, and proliferative granulocytes, day 6.Cell free DC fluid harvested on day 4 from chambers with BMC and mature granulocytes slightly reduced granulocyte formation in new DC cultures, but not more than DC fluid from control cultures (BMC). Mice which had carried chambers of either group for 4 d, were not different when used as hosts for new DC cultures. However, they were less able to stimulate cell growth in DC than untreated mice.Transfer of DC to new host mice on day 2 completely reversed the inhibition by mature granulocytes which otherwise could be detected in numbers of CFU‐C on day 5 and proliferative granulocytes on day 7.However, CFU‐S numbers were reduced on day 5 following reimplantation indicating an increased differentiation of CFU‐S. Transfer of DC as late as day 4, rapidly restored the granulocyte numbers in the granulocyte co‐culture group suggesting that also more differentiated granulocytes were stimulated to further growth and differentiation. It is proposed that factors from mature granulocytes reversibly inhibit myeloid differentiation. Differentiation can again be induced by factors which reach the DC shortly after intraperitoneal implantation i

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1978.tb00387.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

A new case of chronic myeloid leukaemia with a complex translocation involving chromosomes No. 1, 9 and 22 is reported. The striking similarity of this case with another previously reported case and the involvement of band 9q34 in chromosome rearrangements in CML patients indicates clearly a non‐randomness of chromosome abnormalities in this blood disease, even when the translocation is different from the usual t(9;22) one. The usefulness of R‐banding techniques is emphasized in these ca

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1978.tb00388.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Qualitative platelet parameters (volume, 5 hydroxy‐tryptamine (5 HT) organelles studied by the mepacrine test, and density) were evaluated in 31 patients with acute leukaemia: 11 myelomonocytic (AML), 8 lymphoblastic (ALL), 12 granulocytic (AGL). Macrothrombocytosis was observed in most of the cases of AML, was rare in AGL and was never found in ALL. The 5 HT organelles / volume ratio was normal in AGL and ALL but was significantly decreased in AML. In contrast, platelet density distribution was always abnormal whatever the platelet volume and 5 HT organelle concentration. Thus, using simple new methods, convenient even in cases of thrombocytopenia, we demonstrate that qualitative platelet abnormalities are a constant feature in acue leukaemia and that they are more severe in AM

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1978.tb00389.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

The aim of the present work was to investigate the effect of adrenergic alpha‐ and beta‐1‐receptor stimulation on the peripheral platelet count. The experiments were carried out on 8 healthy male volunteers using radioisotopically labelled platelets. 3 subjects received i.v. infusions of adrenaline (0.09 μg × kg‐1× min‐1) before and after the ingestion of 40 mg propranolol. In response to the first infusion there was an instant increase in the venous platelet‐bound radioactivity (PBR) which amounted to 12 % over basal value. This effect of adrenaline seemed to be potentiated by propranolol pretreatment. 5 subjects received i.v. infusions of the highly selective beta‐1‐receptor agonist H 133/22 (prenalterol, Hässle, Sweden). In response to a cumulative dose of 4.75 mg prenalterol a slight but significant (P<0.05) decrease in PBR occurred.It is concluded that alpha‐receptor stimulation causes a depletion of platelets from the exchangeable splenic platelet pool resulting in a concomitant increase in the peripheral platelet count. Beta‐receptor stimulation has an opposite effect on the spleen. The trapping of platelets by the spleen is mediated both via beta‐1‐ and beta‐2‐receptors, but the effect of beta‐2‐recept

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1978.tb00390.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY