摘要:

A patient with idiopathic thrombocytopenic purpura, known for at least 30 years, developed a sclerosing reticulum cell sarcoma following 7 years of immunosuppressive treatment with azathioprine without steroids.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01441.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

4 patients with chronic severe aplastic anaemia and with persistent foci of intense haematopoietic activity, so‐called ‘Hot Pockets’, in their bone marrow were studied. In all patients the remainder of the bone marrow was morphologically and erythrokinetically hypoplastic. The cellular morphology in these ’Hot Pockets’ displayed megaloblastic features and definite maturation abnormalities in erythroblasts, but no consistent changes in the myeloid or megakaryocytic series.The presence of persistent ‘Hot Pockets’ presents a conceptual challenge since these pockets contain multipotential stem cells capable of differentiation and self‐renewal, but obviously incapable of repopulation of the bone marrow. In view of additional evidence for dyserythropoiesis in aplastic anaemia such as changes in erythroblast morphology and the production of macrocytes, fetal haemoglobin and complement sensitive cells, it seems likely that the bone marrow of aplastic anaemia patients is totally dyserythropoietic rather than hypoactive and that bone marrow transplantation in many cases may be both justifie

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01442.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

In vitro culture technique of bone marrow cells has been applied to study the cause of anaemia in uraemic patients on maintenance haemodialysis. Incorporation of59Fe into haem in bone marrow cells of the patients in the presence of erythropoietin, as well as the inhibitory effect of their plasma on the response of normal bone marrow cells to erythropoietin, was examined.Increase in haem synthesis rate by erythropoietin in the bone marrow cells of uraemic patients (n14; 7.9 ± 1.4) was not significantly different from that in normal bone marrow cells (n9; 5.9 ± 1.4, p>0.05), thus indicating the presence of erythroid precursor cells with normal responsiveness to erythropoietin in uraemic patients.On the basis of these results, the humoral inhibitory factor appears to play a significant role in the pathogenesis of renal anaemia, in addition to the low level of circulating erythropoietin.All the plasma from uraemic patients inhibited, in dose‐dependent way, the response of normal bone marrow cells to erythropoietin. Levels of erythropoietin in the plasma samples of uraemic patients were much lower than those of the patients with iron deficiency anaemia with comparable Hb concentrati

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01443.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

A case of pure red cell aplasia appearing 6 months following thymectomy is reported in a 43‐year‐old man. Immunofluorescence studies of the patient's bone marrow have demonstrated the presence of membrane‐bound IgG on the majority of erythroblasts and some mature erythrocytes. It is suggested that the IgG represents anti‐erythroid autoantibodies. The number of Ig‐bearing lymphocytes in peripheral blood was decreased as was the level of immunoglobulins indicating a B‐lymphocyte deficiency. T‐lymphocyte functions were without remarks. The patient was initially treated with corticosteroids and oxymetholone. He responded well to this therapy but relapsed 9 month later. Cyclophosphamide treatment was started and followed by a complete haematolo

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01444.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

In a series of 128 consecutive patients dying from severe megaloblastic anaemia, over half the deaths occurred within one week of hospitalisation and one third were sudden and unexpected. Congestive cardiac failure was extremely common in these patients and was often associated with pleural effusions. Just over half the patients were examined at autopsy and of these 42% had pleural effusions and 15% had pericardial effusions. The cause of the latter is unclear as is their clinical significance, nevertheless this condition has not previously been reported in the megaloblastic anaemias and may well play a part in the fatal outcome in such patients.Blood transfusion was administered to over half the patients, usually in the form of whole blood. It is recommended that transfusions be used sparingly, and consist of cells or preferably exchange transfusion, the latter being of particular value in the elderly or in those with pre‐existing cardiac failur

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01445.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

A patient with multiple myeloma in whom acute erythroleukaemia developed 5 years following treatment with irradiation and melphalan is reported. Immunoglobulin synthesis and immunofluorescence investigations provided evidence that the blast cells in the peripheral blood did not belong to the plasma cell series; ultrastructure examination demonstrated their myeloid origin.Chromosomally abnormal cells were observed in both the bone marrow and peripheral blood.Light‐ and electron microscopy of erythropoiesis in this case showed distinct features of dyserythropoiesis, similar to those described in other entities.The erythroid cell abnormalities are discussed in the light of their being either indications of malignancy or of a reactive proces

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01446.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

The influence of leukaemie cells from 12 patients with acute leukaemia on normal granulopoiesis in agar culture was investigated using leukeamic cell feeder layers. Leukaemie feeder cells from 7 of the 12 patients elicited no colony growth, while cells from the remaining 5 stimulated normal colony growth. In 3 of the 7 non‐stimulatory patients release of inhibitory factors from the leukaemie cells seemed responsible for the effect on normal granulopoiesis, while inappropriate colony stimulating factor (CSF) production by the feeder cells could not be ruled out in the remaining 4 patients.When the leukaemic cells were cultured with, as well as without, conditioned medium, cells from 5 of the patients formed clusters. Growth in these cultures did not correlate to the effect found in the feeder layer experiment

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01447.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

This paper describes the influence of cells capable of releasing colony stimulating activity (CSA) in vitro on the formation of granulocytic colonies by normal human bone marrow in diffusion chambers in mice. A carbonyl iron method was used to remove phagocytic cells from normal human bone marrow. This treatment prevented spontaneous colony and cluster formation when the cells were cultured in agar in vitro at initial concentrations of 2–5 times 105cells per ml. However, non‐phagocytic bone marrow cells formed granulocytic colonies when inoculated into diffusion chambers at 105cells per chamber and cultured in 450 R‐irradiated or non‐irradiated mice. The formation of granulocytic colonies by carbonyl iron treated marrow in diffusion chamber cultures was not consistently enhanced by the admixture of 1.4 times 1051500 R‐irradiated syngeneic light density blood cells (1.077 g/ml) to the inoculum resulted in a slight, non‐significant decrease in the number of colonies in diffusion chambers. The stimulating effect of host irradiation on neutrophilic colony formation was independent of the presence of CSA releasing cells in the cha

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01448.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

We have obtained antisera from rabbits which react with serum from several leukaemia patients after absorption with normal human serum. The specificities of these rabbit antisera have been shown to be closely related to those of the original anti‐LAA antisera of Viza et al (1970) and Harris et al (1971). Thus, the existence of leukaemia associated (but probably not leukaemia specific) serum antigen in man has been confirmed.One of the animals was immunized with normal amniotic fluid obtained at 15–16 weeks of gestation. Thus, LAA seems to be a normal constituent of amniotic fluid. This suggests that LAA is another onco‐fetal comp

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01449.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

2 patients with associated lymphoproliferative disease and pernicious anaemia are described. The lymphocytes in each were characterized by surface marker studies. In 1 of the cases, therapy with vitamin B12appeared to accelerate the neoplastic growth.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01450.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY