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1. |
Application of the Beckman JE6‐B Elutriator System® in the isolation of human monocyte subpopulations |
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Scandinavian Journal of Haematology,
Volume 34,
Issue 1,
1985,
Page 5-8
Richard R. Mason,
Roy S. Weiner,
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摘要:
Beckman Instruments recently developed an improved version of their JE‐6 Elutriator Rotorr̀. At 3000 rpm (867 x g), lymphocytes were eluted from the chamber at flow rates of 10 to 18 ml/min. Small monocytes (362 μ3) were collected at 18 ml/min over 400 ml while the larger monocytes (395 μ3) were retained in the chamber until the flow rate was increased to 28 ml/min. These parameters differ from those previously reported by us when we used Beckman's original commercial version of the JE‐6 Elutriator Rotor. The new JE‐6B rotor required an increase of centrifugal force from 625 to 867 x g generated by an increase in rpm from 2500 to 3000, as well as an increase in flow rates to collect both small (17 to 18 ml/min) and large (26 to 28 ml/min) monocytes. The monocytes collected using the new rotor with the revised protocol have the same physical and functional properties as those collected with the earlier version using our previously published
ISSN:0036-553X
DOI:10.1111/j.1600-0609.1985.tb00735.x
出版商:Blackwell Publishing Ltd
年代:1985
数据来源: WILEY
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2. |
Extramedullary haemopoiesis after bone marrow transplantation |
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Scandinavian Journal of Haematology,
Volume 34,
Issue 1,
1985,
Page 9-12
R. Arnold,
W. Calvo,
B. Heymer,
T. Schmeiser,
H. Heimpel,
B. Kubanek,
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摘要:
44 patients underwent bone marrow transplantation (BMT) for treatment of severe aplastic anaemia or haematological malignancies. During their post‐transplant phase all patients had erythroblasts and granulocytic precursors in their peripheral blood. 15 patients died between day +6 and d +346 after BMT and autopsies were performed. The sections of all 15 patients revealed extramedullary haemopoiesis in the spleen. Extra‐medullary haemopoiesis in the liver was found only in those patients who died early (between d +6 and d +21 after BMT). Medullary haemopoiesis, normally only occurring in the vertebral body, was also observed in the shaft of the femur.The present data show that after BMT all tissues with a haemopoietic matrix in ontogenesis can be repopulated with haemopoiesis in the early phase of reconstitution, possibly to compensate for the haemopoietic insufficiency after conditioning therapy. The expansion of haemopoiesis in the later period of up to 1 year after BMT, remains to be explai
ISSN:0036-553X
DOI:10.1111/j.1600-0609.1985.tb00736.x
出版商:Blackwell Publishing Ltd
年代:1985
数据来源: WILEY
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3. |
Remission of acquired pure red cell aplasia following plasma exchanges: |
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Scandinavian Journal of Haematology,
Volume 34,
Issue 1,
1985,
Page 13-15
Abderrahim Khelif,
Huan Vu Van,
Jean Pierre Tremisi,
Florence Alfonsi,
Daniel Perrot,
Jean Motin,
Jean Jacques Viala,
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摘要:
A 22‐year‐old woman had an idiopathic pure red cell aplasia that failed to respond to high doses of corticosteroids. After a series of 10 plasma exchange procedures, bone marrow erythropoiesis and reticulocyte blood count returned to normal; the haematological remission has been now persistent for 12 months. The place of plasma exchange in the management of pure red cell aplasia and its mode of action will be discus
ISSN:0036-553X
DOI:10.1111/j.1600-0609.1985.tb00737.x
出版商:Blackwell Publishing Ltd
年代:1985
数据来源: WILEY
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4. |
Immunological markers in acute leukaemia |
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Scandinavian Journal of Haematology,
Volume 34,
Issue 1,
1985,
Page 16-21
S. Avnstrøm,
E. Ralfkier,
N. Clausen,
T. Plesner,
N.E. Hansen,
N.I. Nissen,
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摘要:
61 acute leukaemias were classified by both immunological and morphological criteria. In 58 (95 %) of the cases correlation was found between the immunological and morphological diagnosis. In 3 (5 %) cases, no correlation was found between the immunological subclasses and the FAB‐subgroups in ALL. Neither was a correlation found between FAB‐subclasses in AML and reaction with the myeloid antibodies other than MY‐7 which did preferentially react with M4and M5leuk
ISSN:0036-553X
DOI:10.1111/j.1600-0609.1985.tb00738.x
出版商:Blackwell Publishing Ltd
年代:1985
数据来源: WILEY
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5. |
Kinetic evaluation of four Factor VIII concentrates by model‐independent methods |
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Scandinavian Journal of Haematology,
Volume 34,
Issue 1,
1985,
Page 22-28
M. Matucci,
A. Messori,
G. Donati‐Cori,
G. Longo,
S. Vannini,
M. Morfini,
E. Tendi,
P. L. Rossi‐Ferrini,
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摘要:
Single‐dose kinetics of 4 Factor VIII concentrates (Kryobulin, Hemofil T, Koate, cryoprecipitate) were studied in 41 patients with haemophilia‐A. Model‐independent methods were adopted for calculating the kinetic parameters (area under the curve, clearance, area under the moment curve, mean residence time, volume of distribution at steady‐state). No substantial difference was observed in the kinetic characteristics of the 4 Factor VIII concentrates. A considerable interindividual variability of the calculated kinetic parameters was demonstrated for all concentrates. Our findings support the need to individualize Factor VIII
ISSN:0036-553X
DOI:10.1111/j.1600-0609.1985.tb00739.x
出版商:Blackwell Publishing Ltd
年代:1985
数据来源: WILEY
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6. |
A possible pathogenetic role of cationic proteins (CP) released by stored granulocytes in the development of pulmonary infiltrates after granulocyte transfusions |
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Scandinavian Journal of Haematology,
Volume 34,
Issue 1,
1985,
Page 29-34
D. Campana,
G. Camussi,
L. Bergui,
P. Vallauri,
L. Tesio,
C. Tetta,
F. Caligaris‐Cappio,
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摘要:
The cationic protein (CP) content of polymorphonuclear neutrophils (PMN) prepared for transfusion is depleted after storage. The supernatants from these PMN have in vitro a PMN aggregating activity which is abolished by the preabsorption with a specific rabbit anti‐human PMN CP serum. Furthermore, when the supernatants stored for few hours were injected into New Zealand White rabbits, a marked sequestration of PMN took place in the lung microvascular bed. It is suggested that PMN storage per se can cause the release of intracellular mediators of possible pathogenetic importance in the development of the pulmonary infiltrates observed after PMN transfusion
ISSN:0036-553X
DOI:10.1111/j.1600-0609.1985.tb00740.x
出版商:Blackwell Publishing Ltd
年代:1985
数据来源: WILEY
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7. |
Amelioration of hydroxyurea‐induced suppression of phagocytosis in human granulocytes by free radical scavengers |
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Scandinavian Journal of Haematology,
Volume 34,
Issue 1,
1985,
Page 35-38
Izabella Szczepańnska,
Jolanta Kwiatkowska,
Waldemar M. Przybyszewski,
Ewa Sitarska,
Janina Malec,
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摘要:
The exposure of human circulatory white cells in vitro to 0.1 — 1 — 10 mol/l hydroxyurea (HU) for 20 h induced a progressive dose‐dependent suppression of the phagocytic activity of granulocytes. The suppressing effect of 20 h exposure to 1 mol/1 HU was used to examine the protection afforded by free radical scavengers against HU‐induced cytotoxicity. It has been found that, in the suitable concentration of the protecting agent, a substantial protective effect of sodium benzoate, acetylosalicylic acid, α‐tocopherol, ascorbic acid, catalase, peroxidase or superoxide dismutase can b
ISSN:0036-553X
DOI:10.1111/j.1600-0609.1985.tb00741.x
出版商:Blackwell Publishing Ltd
年代:1985
数据来源: WILEY
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8. |
Kinetics and in vivo distribution of 111‐In‐labelled autologous platelets in chronic hepatic disease: mechanisms of thrombocytopenia |
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Scandinavian Journal of Haematology,
Volume 34,
Issue 1,
1985,
Page 39-46
Kai Gjerløff Schmidt,
Jens Wæver Rasmussen,
Claus Bekker,
Poul Erik Rørbæk Madsen,
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摘要:
The kinetics and distribution in vivo of autologous 111‐In‐labelled platelets were studied in 20 patients with chronic hepatic disease. The patients, 16 of whom were thrombocytopenic, exhibited a shortened platelet mean life time, a reduced platelet recovery and a normal platelet turnover, the latter 2 of which were positively correlated to the platelet count. Platelet in vivo recovery was negatively correlated to the spleen volume. In accordance with this, scintigraphic studies revealed that the spleen was the major organ of platelet sequestration and destruction, the role of the liver being almost negligible. Signs of platelet destruction in the bone marrow were also found. Our results indicate that splenic platelet pooling and accelerated platelet destruction, accompanied by inability of the bone marrow to compensate for the thrombocytopenia are the main causes of the thrombocytopenia accompanying chronic hepatic dise
ISSN:0036-553X
DOI:10.1111/j.1600-0609.1985.tb00742.x
出版商:Blackwell Publishing Ltd
年代:1985
数据来源: WILEY
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9. |
Kinetics and distribution in vivo of111In‐labelled autologous platelets in idiopathic thrombocytopenic purpura |
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Scandinavian Journal of Haematology,
Volume 34,
Issue 1,
1985,
Page 47-56
Kai Gjerløff Schmidt,
Jens Wæver Rasmussen,
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摘要:
The kinetics of autologous111In‐labelled platelets were studied in 26 patients with ITP. The platelet mean life time (MLT) was considerably shortened, the platelet in vivo recovery slightly lowered and the platelet turnover normal. Comparative studies of the kinetics of simultaneously injected111In‐ and51Cr‐labelled platelets in 10 patients showed the MLT and turnover of51Cr‐platelets to be shorter and higher, respectively, than those of111In‐platelets, suggesting that51Cr‐labelling in ITP may underestimate platelet MLT and overestimate platelet turnover. Our results confirm that accelerated platelet destruction is an important pathogenetic factor in ITP, and that the platelet concentration may be influenced by increased splenic platelet pooling and by inability of the bone marrow to respond adequately to the low platelet count. Our scintigraphic studies showed that the spleen played an important role for platelet destruction in most patients, with the liver contributing in so
ISSN:0036-553X
DOI:10.1111/j.1600-0609.1985.tb00743.x
出版商:Blackwell Publishing Ltd
年代:1985
数据来源: WILEY
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10. |
T‐cell chronic lymphocytic leukaemia: Anomalous cell markers, variable morphology, and marked responsiveness to pentostatin (2′‐deoxycoformycin) |
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Scandinavian Journal of Haematology,
Volume 34,
Issue 1,
1985,
Page 57-67
A. S. D. Spiers,
M. P. Davis,
Marilyn Levine,
C. Y. Li,
K. F. Mangan,
J. J. Mazza,
L. T. Neu,
J. O'Brien,
A. E. Rauch,
J. C. Wiltsie,
Leslie Bieler,
Carol A. Lorch,
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摘要:
4 men with an unusual variant of T‐cell chronic lymphocytic leukaemia are reported. The clinical features differed from the virus‐associated T‐cell disease reported from Japan, the Caribbean, and the southeastern United States. Cytology was pleiomorphic: cells with cerebriform nuclei resembling Sézary cells and lymphocytes with cytoplasmic granules resembling T‐suppressor cells occurred in the same patients. Immunofluorescence studies with monoclonal antibodies suggested that the leukaemia cells expressed determinants of both helper‐ (OKT4+) and suppressor‐(OKT8+) related antigens. The cells were TdT‐negative. In all patients the disease was very refractory to conventional cytotoxic agents, but there was prompt and extensive response to the adenosine deaminase inhibitor pentostatin (2′‐deoxycoformycin). This agent merits further study in the treatment of T‐cell chronic l
ISSN:0036-553X
DOI:10.1111/j.1600-0609.1985.tb00744.x
出版商:Blackwell Publishing Ltd
年代:1985
数据来源: WILEY
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