摘要:

A controlled environmental culture system in which consistent growth of fetal liver cells typical of the tissue of origin and capable of producing erythropoietin in culture is described. This system attempts to duplicate the major components of the natural habitat of the mammalian cell allowing for meaningful studies to be conducted on the regulation and control of erythropoietin biosynthesis. The direct effects of changes in pO2, pCO2, pH and various cell nutrients on cells producing erythropoietin is now possible.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01133.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

Hb and PCV values were studied in three groups of Addis Ababa women during their last month of pregnancy and compared with those in a control group of non‐pregnant Addis Ababa women. Irrespective of socio‐economic group and parity, the Hb and PCV values were almost identical in all four groups.In one of the three test groups, a group of ‘non‐privileged’ multiparae, S‐iron, S‐transferrin (TIBC), S‐folate and S‐vitamin B12were also examined and showed normal values.These findings indicate a rarity not only of true anaemia but also of ‘physiological anaemia of pregnancy’. This latter may not, therefore, be an adaptive phenomenon of regular occurrence as has usually been assumed hitherto. The explanation for the aberrant haematological behaviour in pregnant women living in highland Ethiopia is probably the lifelong exposure to a very high iron intake combined with hypoxia due

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01134.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

The hypothesis that transcobalamin I (TC I) originates from granulocytes was tested by comparing the isoelectric focusing (IEF) patterns of the R‐type binder (cobalophilin) of vitamin B12of: 1) lysed granulocytes, 2) 24 h granulocyte output, and 3) plasma. The preparations came from the blood of 5 normal subjects and 4 with myeloproliferative states. The cobalophilin released into a culture of granulocytes resembled the binder in the granulocytes initially. TC I, defined as the α1cobalophilin with components isoelectric between pH 2.9‐3.35 and as a carrier of native plasma B12, was not released by granulocytes. The granulocyte binder of leukaemic granulocytes did not differ from the normal in content per cell or amount rele

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01135.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

Peculiar erythroblasts with multiple vacuoles were observed in the bone marrow of an erythroleukaemic patient.Some erythroblasts contained more than 80 vacuoles on a thin section. The contents of vacuoles were amorphous with low electron density. No transitional form from mitochondria or other organelles was observed. Since these vacuolated erythroblasts were clearly distinguishable from megaloblastoid erythraemia cells, multiple vacuoles formation is presumably attributable to the disturbance of microconnection net work in the normal red cell series.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01136.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

Erythroid regeneration was studied in lethally irradiated mice given transplants containing equivalent numbers of haemopoietic stem cells (i.e. CFU) from fetal liver, neonatal marrow or adult marrow. Adult marrow was taken from normal control mice, whose CFU for the most part were not in active cell cycle, as well as from phenylhydrazine‐treated groups whose CFU were in similar state of proliferation (i.e. ˜40–50% in DNA synthesis) as those derived from fetal liver and neonatal marrow. Splenic and femoral radioiron (59Fe) incorporation were measured at intervals after transplantation and were found to begin earliest in mice given fetal liver, then in animals given neonatal marrow and latest in recipients of adult marrow. Peripheral reticulocytes showed a similar pattern of recovery. The data reported herein suggest that the differences in erythroid regeneration evoked by transplants of fetal liver, neonatal marrow or adult marrow, are not solely attributed to the degree of proliferation in the pluripotential stem cell compartment. These data may, however, suggest a shorter doubling time for cells comprising the fetal and newborn committed erythroid compartm

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01137.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

A rapid method for measuring volume distributions of human, calf and goat lymphocytes and their nuclei is described along with the type of quantitation these measurements can provide by computer analysis. The size distribution studies indicate the presence of two populations of lymphocytes and their nuclei irrespective of the cell source. It is suggested that proliferative fractions of various cell populations may be estimated by determining the nuclear volume distribution.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01138.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

Inherited, acquired and iatrogenic ‘diseases of low antithrombin activity’ share a common denominator: each is associated with an increased tendency toward, or incidence of, thrombosis. This suggested the possibility that these diseases constituted a distinct pathogenetic entity.Molecular models and clinical studies suggested 3 interrelated phenomena: increased serum procoagulants; decreased antithrombin; a tendency to thrombosis. The term ‘Acquired Thrombophilia’ is proposed to designate pre‐thrombotic and thrombotic individuals presenting this non‐inherited combination of laboratory and clinical findings.The antithrombin clotting time is shown to be inversely related to the level of circulating serine procoagulants, directly related to the level of antithrombin, and results from a procoagulant‐antithrombin inter‐molecular reaction. Patients with Acquired Thrombophilia have ‘turned‐on’ or excessive procoagulants resulting in ‘turned‐off’ or tightly b

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01139.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

A 27‐year‐old woman with proteinuria and normal serum creatinine for 10 years developed chronic lymphocytic leukaemia (CLL) and after further 2 years during treatment with prednisone and chlorambucil a malignant histiocytoma appeared. Free μ‐chains were demonstrated in serum, but it is possible that μ‐chain disease (μ‐CD) and Bence‐Jones proteinuria had persisted unrecognized for 12 years, since vacuolated plasma cells were found when the primary bone marrow smear was re‐evaluated and no glomerular disorders were found. The clinical findings from the now 11 known cases of μ‐CD are reveiwed and the immunochemical findings are reported in part II (Axelsen et al 1976).The patient's mother died of acute leukaemia at the age of 24. The mother's sister has mammary carcinoma and several members of the mother's line apparently have increased tendency to infections. Of 27 family members, none exhibited immunoglobulin defects, but 10 members of the mother's line had B‐ as well as T‐lymphocytosis and 5 m

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01140.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

Agarose gel electrophoresis of serum from the eleventh case of μ‐chain disease (μ‐CD) showed two distinct abnormal bands due to free light chains andk‐complexes with other serum proteins. This caused diagnostic difficulties on conventional immunoelectrophoresis, and crossed immunoelectrophoresis with intermediate gel is demonstrated as a superior tool for investigation of μ‐CD. A simple rocket Immunoelectrophoresis method for μ‐CD screening is also shown. The μ‐CD protein had α‐2‐mobility and a part of the protein carriedk‐determinants probably due to secondary binding. Preparative ultracentrifugation and size chromatography on serum demonstrated that the μ‐CD protein was present in serum in several sizes, the smallest being a dimer of μ‐chains. Due to scarcity of material the abnormal protein could

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01141.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

An evaluation of 433 children with thrombocytopenia is presented. The material comprises all cases diagnosed in Denmark during the period 1959–1969. The incidence was found to be 31.9 cases per 1,000,000 inhabitants 15 years of age per annum with a peak at the age of 3 to 4 years; the majority of the cases was diagnosed in the spring. Preceding infection, usually associated with fever and localized in the upper respiratory tract, was the most common cause. The majority of the congenital cases was hereditary and associated with other malformations involving in particular the immune system. In about half of the cases the course was spontaneous and no treatment was necessary; the remaining patients were treated with glucocorticoids, including 46 patients, who had undergone splenectomy. 22 patients died, including 16 cases due to serious underlying disorders; in 6 cases the cause of death was haemorrhage (mortality rate = 1.4%). At the follow up the platelet count was below 50 mia/1 in 14 patients (4%). No difference was found concerning the platelet count at the follow up between the patients with spontaneous recovery, the patients treated with glucocorticoids and the patients, in whom splenectomy had been performed. During the course the disease autoimmune haemolytic anaemia occurred in 2 patients, whereas no patient developed systemic lupus erythematosu

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01142.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY