ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb02202.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

An increased sialic acid content of the fibrinogen molecule is found in foetal fibrinogen and as an acquired disorder in hepatic disease. A qualitatively abnormal fibrinogen was detected in the plasma of a 25‐year‐old man with a thrombotic tendency. The purified fibrinogen had a significantly increased content of sialic acid, an abnormal fibrinmonomer polymerization, and a changed mobility in crossed affino‐immunoelectrophoresis using immobilized helix pomatia lectin. The patient had no biochemical or clinical signs of liver disease. The occurrence of a thrombotic tendency and an increased fibrinogen sialic acid content without signs of liver disease may represent a new variant of congenital dysfibrinogen

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb02203.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

19 patients with myelofibrosis, primary or following polycythaemia vera were studied cytogenetically. Bone marrow cells, unstimulated and stimulated cells from peripheral blood were investigated. 7 patients were found to have clonal aberrations, 3 of whom had a structural rearrangement of chromosome 13. In 2 additional cases single mitoses with 13q‐ were found. Reviewing the files on patients previously studied in our laboratory, 2 more patients with 13q‐ markers were noted. Both had had haematologic disorders in which fibrosis of the bone marrow can be found, but this feature could not be evaluated retrospectively, because no biopsies had been taken. Our data and those found in the literature suggest that rearrangements of 13q12 → q22 are often associated with myelofibrosis, both in its primary form or following polycythaemia

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb02204.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

The records of 91 patients with multiple myeloma were reviewed and staging was performed according to Durie&Salmon (1). Patients with progressive disease were re‐staged. Patients first diagnosed in stage I were younger than patients first diagnosed in stage III and survived longer than patients in stages II or III. The duration of stage I was longer than the duration of stages II and III. When patients in stage I‐II progressed into stage III, their age and remaining survival time were similar to the age and survival of patients first diagnosed in stage III. These findings are compatible with the hypothesis that myeloma stage I prededes myeloma stage III and is often diagnosed by chance. It is suggested that patients with myeloma stage I should be omitted from comparisons between different myeloma populati

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb02205.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

36 acute leukaemias in children, 24 lymphoblastic and 14 myelogenic, have been examined with a set of 10 monoclonal antibodies by indirect fluorescent staining. In the lymphoblastic group the leukaemic cells of 4 children were found to have T‐cell phenotype, while 19 of the other 20 T‐cell phenotype negative cases were found to be positive for the c‐ALL antigen. All 20 were negative for surface immunoglobulin as well as cytoplasmic μ‐heavy chains. However, 17 (85 %) reacted positively with the monoclonal antibody AB‐1 which we have developed against a B‐cell lymphoma, thus revealing B‐lineage specificity. Another B‐lineage‐associated antibody (AB‐2) reacted with 8/20 (40 %) of the cases with distribution similar to B‐1. These findings suggest that the great majority of non‐T‐non‐B acute lymphoblastic leukaemias are neoplasms derived from the B‐cell progenitor compartment. Moreover, monoclonal antibody testing allows further sub‐categorization in this group. Similarly the acute‐myelogenic leukaemia group could be subdivided into phenotypic subsets. The importance of using panels of monoclonal antibodies in the diagnosi

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb02206.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Whereas haemolytic anaemia is commonly encountered in infants and young children with cytomegalovirus (CMV) infections, it is an infrequent complication of CMV‐induced infections in previously healthy adults. The data from 2 such patients are presented. One patient's Hb fell to a level of 36 g/l, and she required prednisone and blood transfusions. Her direct antihuman globulin test (DAT) was positive (IgG), and her red blood cell survival (51Cr) revealed a T1/2 of 5 d. Both saline‐agglutinating and low‐molecular‐weight cold agglutinins (CA) (4°C) that reacted against both cord and adult cells were identified. In the second case, a moderate haemolytic anaemia (lowest Hb 87 g/l) was accompanied by negative DAT and CA studies. 20 other patients with CMV‐mononucleosis were evaluated for evidence of subclinical haemolysis. Reticulocyte counts>3.0% were noted in 9 of these patients. Haptoglobin values were below 0.5 g/l in 13 patients, and a positive DAT was recorded in 3/10 cases. This study documents haemolysis in many non‐immunosuppressed adult patients with CMV infections. The mechanism responsible rem

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb02207.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Iron metabolism during inflammation was studied in normal and stimulated peritoneal mouse macrophages. A model was developed for detection of ferritin synthesis in these cells, and inflammatory stimulation was produced by i.p. injection of thioglycollate broth or i.m. injection of turpentine. The cells were adhered to culture discs and incubated at 37°C with59Fe‐transferrin and3H‐leucine, and after washing the cell lysate was chromatographed. In the lysate from stimulated macrophages iron and tritium activity was found in a joint peak, and both were precipitated by a specific anti‐mouse ferritin antibody. No significant peaks of radioactivity were found in lysates from normal cells. This showed that inflammatory RES cells have an increased ferritin synthesis. The uptake of59Fe was investigated in a separate study and the stimulated macrophages were found to have a much higher iron uptake than normal macro

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb02208.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

The turnover of human tissue‐type plasminogen activator (t‐PA), purified from cell culture fluids and radiolabeled with125I, was studied in 2 healthy males. After injection, the plasma radioactivity initially disappeared with a half‐life of about 3 min, but after 30 min reached a plateau level which persisted for several hours. The radioactive material had then already been converted to forms soluble in 10% trichloroacetic acid (TCA). Surface counting rates indicated a rapid uptake in the liver during the first 10 min after injection, followed by a rapid release from the liver, beginning already at 25 min, which coincided with the appearance of the TCA‐soluble radioactive material. After 18 h, about 80% of the injected dose had already been excreted in the urine. We conclude that, in humans, injected t‐PA is rapidly cleared from the circulation, mainly by the liver, and within 30 min metabolized to low molecular weight forms which ultimately (within 1 d) appear in

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb02209.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

The rare combination of sickle cell trait with hereditary spherocytosis in a Sudanese family is reported. The splenic hypofunction and fibrosis as a result of sickling has ameliorated the clinical course of the 35 year old father. The symptoms of a six years old son seem to be getting milder with age too. The family is managed conservatively and splenectomy was not required.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb02210.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

The prognostic value of different pretreatment laboratory and clinical findings at diagnosis was assessed in a series of 141 patients with generalized non‐Hodgkin's lymphoma. Univariate and multivariate survival analysis (Cox's regression model) was performed, using serum analysis of deoxythymidine kinase (S‐TK), β2‐microglobulin, lactic dehydrogenase, α1‐acid glycoprotein = orosmucoid (S‐α1AGP), haptoglobin and ferritin. In addition, Hb and the erythrocyte sedimentation rate (ESR) were measured. The clinical variables were age, presence or absence of B‐symptoms, histopathology (‘low‐grade’; ‘intermediate grade’ and ‘high‐grade’ malignancy) and bone marrow involvement. Of the 8 biochemical markers, all except Hb and the ESR showed a significant relationship to survival. Among the clinical variables, this finding was made for B‐symptoms and histopathology. Using a multivariate analysis on all variables, S‐TK was found to be the best factor for predicting duration of survival. The only significant additional information was provided by S‐α1AGP. When only the clinical variables were taken into account, it was found that histopathology added significant information to that yielded by B‐symptoms in the prediction of the survival time. When the biochemical variables were added to this model, only S‐TK was o

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb02211.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY