摘要:

The electrophoretic mobility of erythrocytes from 3 patients with PNH was measured by standard techniques. Erythrocyte mobility is a measure of the net negative surface charge of these cells. In each of the 3 patients studied the mobility was significantly increased over normal cells indicating that there is an increase in negative surface charge on PNH erythrocytes. Following the Ham's and sucrose lysis tests, surviving cells showed mean mobilities that were not statistically different from normal cells. This suggests that these cells with higher surface charge are vulnerable to complement lysis.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01144.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

A patient with leiomyomata of the skin and erythrocytosis is reported. After surgical removal of the larger skin tumours there was remission of the haematologic abnormalities and the erythrocytosis and the red cell mass decreased to normal levels. The raised serum erythropoietin levels were reduced but remained slightly elevated and less than those recorded prior to surgery. Tumour extracts contained significant erythropoietic activity. This seems to be the first documented case of erythrocytosis associated with skin leiomyomata.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01145.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

Two patients with mild chronic haemolytic anaemia, a mother and her son, were found to be heterozygous for erythrocyte pyruvate kinase deficiency.In the red blood cells the enzymatic activity was reduced by about 50 % and the residual PK had normal kinetic properties, stability and electrofocusing pattern. The PK antigen concentration was also decreased by half, so that the ratio of the enzymatic activity to the immunological reactivity (i.e. the molecular specific activity) was normal.In the son's liver PK enzymatic activity was slightly reduced and, above all, an abnormal active form, more anodic than normal PK, was detected by electrofocusing. The propositus's liver PK was also slightly thermo‐unstable.It is suggested that the patients were heterozygous for an unstable PK variant which is found in liver, nucleated tissue actively synthesizing proteins, but which disappeared from the erythrocytes because of its unstabilit

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01146.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

24 patients with Philadelphia chromosome positive chronic myeloid leukaemia (CML) in blast crisis were treated with intensive chemotherapy. 16 patients showed either partial or complete response to this treatment, but median survival remained short (13 weeks), and much of this time was spent in hospital. These results were not significantly better than those obtained by others using vincristine and prednisolone alone, and this combination of drugs can often be given on an outpatient basis. It is concluded that until more effective intensive therapy becomes available patients in CML blast crisis should be managed in such a way that the quality of life is not impaired; and that at present vincristine and prednisolone appears to be the most appropriate initial treatment, though even this is far from satisfactory.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01147.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

Calcium ions induce retraction of reptilase clots. This could also be induced by thrombin. Heparin inhibited the reptilase clot retraction induced either by thrombin or calcium, but did not influence the clot retraction induced by ADP. This indicated that the clot retraction induced by Ca2+ was mediated by an activation of the coagulation system with the formation of thrombin.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01148.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

Human blood platelets were separated into 4 populations by centrifugation on a sucrose gradient. Serotonin uptake expressed against cell protein content is the greater as platelets become lighter. The relation between these facts and ATP content in the various platelet populations is discussed.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01149.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

Preincubation of unsensitized sheep erythrocytes with 25 μg/ml of the polycations DEAE‐dextran or polybrene prevented immune haemolysis. This inhibition could be reversed by polyanion. When already sensitized cells were incubated in polycation or when polycation was added to complement, less effect was found on haemolysis. Cell electrophoresis and immunoelectrophoresis showed that polycation does not prevent atachment of antibody to the cel

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01150.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

The presence of FDP in the urine, known to be a sensitive indicator of various kidney disorders, was studied in 14 well‐trained men participating in a 70‐km cross‐country ski race.None of the urine samples contained FDP on the day before the race. Immediately after the race, 4 samples contained FDP. High molecular weight FDP were found in 2 of them, whereas 2 others contained low molecular weight products only. 2 days after the race, 1 of these subjects still had FDP in the urine. In addition, 2 newly FDP positives were observed.Serum FDP were slightly elevated in 2 subjects before the race, and in another subject immediately after the race.The presence of urinary FDP did not correlate either with urinary albumin or uromucoid, or with serum FDP. A drop in plasma fibrinogen immediately after the race was noted in all subjects.It is suggested that the present observations may reflect a transient hyperproteolysis (coagulation and fibrinolysis) in the glomerular circulation, including fibrin formation and dissolution, associated with a transient damage of glomerular capill

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01151.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

Some new techniques for estimating B‐ and T‐lymphocytes in lymphoid tissue are introduced: The density of marker erythrocytes on closed chamber incubated sections was estimated by the light transmission in relation to the number of the respective lymphocyte subpopulation, measured on suspensions of eluted lymphocytes. Using51Cr labelled marker erythrocytes, the number of adsorbed marker erythrocytes per lymphocyte could be estimated from the γ‐irradiation of the section. The number of lymphocytes in the section was determined by the amount of protein in the section compared with the protein amount of a tissue specimen of known wet weight and known lymphocyte content. Furthermore, the number of adsorbed marker erythrocytes per rosette in suspensions of eluted lymphocytes was estimated by means of radioactively labelled marker erythr

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01152.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

A study of membrane receptors on peripheral blood mononuclear cells from a patient with acute monocytic leukaemia is presented. Fc and (C) receptors which are found on normal monocytes were strongly expressed on the leukaemic cells. Electron microscopy and the cultural characteristics of these cells are also described.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01153.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY