摘要:

57Co‐labelled vitamin B12bound to transcobalamin I, transcobalamin II or non‐protein bound, has been mixed with serum from patients with pernicious anaemia treated with long acting vitmain B12preparations. By paper electrophoresis, immunoelectrophoresis and Sephadex G‐200 gel filtration, an antibody of the γG‐globulin type reacting with transcobalamin II was demonstrated. By immunoelectrophoresis the antibody was demonstrated in 8 of 19 patients. Gel filtration experiments showed that the affinity of the antibody to TC II is very sensitive to changes in pH, ionic strength and tem

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1968.tb01712.x

出版商:Blackwell Publishing Ltd    

年代:1968

数据来源: WILEY

摘要:

A large family with a congenital bleeding disorder in 16 of its members was investigated. The disease was dominantly inherited, and the symptoms were ready bruisability, tendency to nose bleeding and bleeding after tooth extraction and operation. The Ivy bleeding time was always, and the Duke bleeding time occasionally, prolonged. The platelet adhesiveness as tested in Hellem's whole blood method was decreased and mostly also in Hellem's plasma‐ADP method and Salzman's method. Spontaneous aggregation in platelet‐rich citrated plasma was absent even when the plasma was placed on a glass slide. After suspension in 3.8 per cent citrate solution the platelets showed an abnormal swelling. The prothrombin consumption test was occasionally pathologic. Factor VIII and other coagulation factors were normal. Moderately severe thrombasthenia is suggested as an appropriate name for the dise

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1968.tb01713.x

出版商:Blackwell Publishing Ltd    

年代:1968

数据来源: WILEY

摘要:

The clinical and laboratory features of a patient with the obstetric defibrination syndrome are described. The abnormal bleeding responded to fibrinogen therapy and epsilon‐aminocaproic acid was not used. The patient's plasma contained little fibrinogen capable of clotting without protamine and it inhibited the thrombin clotting time of normal plasma. Increased fibrinolysis was not observed. Serum obtained from the plasma after addition of protamine and concentrated thrombin contained a substance which reacted with an anti‐fibrin serum when tested by an immunoelectrophoretic technique. The results confirmed that ‘altered’ incoagulable fibrinogen determinants were present in the patient's blood which may have interfered with the thrombin‐fibrinogen reaction. It is suggested that these changes may be due to intravascular coagulation and not only to fibrinogenolysis. Similar but less severe coagulation changes were observed in the infant's c

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1968.tb01714.x

出版商:Blackwell Publishing Ltd    

年代:1968

数据来源: WILEY

摘要:

Following treatment with a vitamin B12preparation with retarded effect, extremely high serum vitamin B12values were observed in several cases. The explanation for this phenomenon is a very high serum vitamin B12‐binding capacity, caused by a vitamin B12binding protein located in the γGarea – presumably an anti

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1968.tb01715.x

出版商:Blackwell Publishing Ltd    

年代:1968

数据来源: WILEY

摘要:

Acute renal failure is reported during a haemolytic crisis in a boy with hereditary spherocytosis since this complication has not previously been published.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1968.tb01716.x

出版商:Blackwell Publishing Ltd    

年代:1968

数据来源: WILEY

摘要:

Plasma clearance of intravenously administered57Co vitamin B12in four patients with fish tapeworm anaemia in relapse was slow in all cases when compared to normal subjects. When labelled vitamin was pre‐bound to normal serum and injected into these patients the clearance pattern became normal. The ability to correct clearance was lost when57Co B12and normal serum were heated to 56° C. for 30 minutes. Addition of an amount of normal serum equal to heated57Co B12serum failed to correct slow clearance in two of three cas

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1968.tb01717.x

出版商:Blackwell Publishing Ltd    

年代:1968

数据来源: WILEY

摘要:

The influence of temperature, pH, incubation medium, and incubation time on the uptake of51Cr by platelets was studied. The uptake of51Cr proceeded for at least 4 hours, most rapid in a modified Ringer solution. The rate of uptake increased with temperature up to 37° C., with platelet number up to 1–2 times 106/μl. and with concentration of51Cr, but was reduced by a surface‐active agent Triton WR 1339.Based on these observations, a modified technique for51Cr‐labelling of platelets is presented. Provided the platelet count is normal, removal of only 120 ml. of blood from the patient is necessary. Even in severe thrombocytopenia, survival of autologous platelets can be estimated by the present

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1968.tb01718.x

出版商:Blackwell Publishing Ltd    

年代:1968

数据来源: WILEY

摘要:

The unsaturated serum vitamin B12binding capacity (UB12BC) of transcobalamin O, I and II (TC O, I and II) of 50 normal subjects was determined by gel filtration on Sephadex G‐200. TC O bound from 0 to 76 pg. per ml. of serum, accounting for up to 5.2 per cent of the UB12BC, while TC I bound from 72 to 1057 pg. per ml., or up to 47.1 per cent of the UB12BC. TC II bound from 650 to 1891 pg. per ml., accounting for up to 90.3 per cent of the UB12BC.There was a significant positive correlation between the binding capacity of TC I and of TC II when each was compared with the total unsaturated B12binding capacity. A positive correlation therefore existed between the binding capacity of TC I and that of TC II.TC O, I and II were the only binders of added B12over a range from 75 to 20,760 pg. per ml. of serum. The relative amount of B12bound to TC I and to TC II remained fairly constant in an individual serum regardless of the amount of B12adde

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1968.tb01719.x

出版商:Blackwell Publishing Ltd    

年代:1968

数据来源: WILEY

摘要:

The purpose of the investigation was to elucidate possible changes in coagulation and fibrinolysis during induced dehydration with Furosemide in 11 normal individuals. The 7 were followed before and for 31/2–4 hours after 80 mg. Furosemide perorally, the last 4 were control patients examined similarly, but with placebo instead of Furosemide. The following changes were observed in all the test subjects:1. Significant increase in the fibrinolytic activity of plasma euglobulin 11/2–2 hours after Furosemide.2. Significant increase in plasminogen and fibrinogen concentration.3. Slight, not significant, increase in urokinase inhibitor. No changes in plasmin inhibi

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1968.tb01720.x

出版商:Blackwell Publishing Ltd    

年代:1968

数据来源: WILEY

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1968.tb01721.x

出版商:Blackwell Publishing Ltd    

年代:1968

数据来源: WILEY