摘要:

Groups of mice have been autopsied at regular intervals during the period of lymphomyeloid tissue regeneration which follows the phase of hypocellularity induced by the i.v. injection of 100 μg (4 mg/kg bodyweight) nitrogen mustard. The marrow cellularity recovered to levels in the normal range by the 8th day and remained in this range up to the 40th day. Subsequently, the marrow showed a slight degree of hypocellularity up to day 120. Granulocytes were predominant during the initial phase of marrow regeneration from days 5–12. The thymus, lymph nodes, and spleen commenced regeneration during the second week post‐injection. The thymus exhibited periodic size variations such that it was substantially larger than the thymus of age‐matched controls from 20–30 d, 46–73 d, and 75–100 d after injection. The lymph nodes and lymphoid tissue of the spleen regenerated only slowly to reach control values by 40–50 d. Superimposed on the recovering lymphoid tissue of the spleen was a phase of erythroid hyperplasia lasting from 10–18 d post‐injection. This coincided with a shift from granulocytosis to erythroid hyperplasia in the marrow. This erythroid hyperplasia lasted until day 30 when the cellular composition of the marrow and spleen returned to normal. A possible explanation of these results is that nitrogen mustard introduces a degree of synchrony into stem cell proliferation and differentiation. Additionally, these results emphasize the role of the haematopoietic microenvironment in the control of stem ce

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb02702.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

A patient with hepatocellular carcinoma, megaloblastic anaemia and increased concentration of serum cobalamin is described. Plasma TC I was increased to 10,000 times the normal concentration, thus explaining the increased concentration of serum cobalamin and a false Schilling test. The increase in plasma TC I in concurrence with undetectable amounts of plasma TC II was a likely explanation for the anaemia. The electron microscopic picture of the hepatocellular carcinoma was in accordance with TC I being produced by the tumour cells.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb02703.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

This report demonstrates a previously disregarded source of error in the count of lymphocytes that have been fixed in Türk's dilution fluid (acetic acid and gentian violet). If the dilution fluid does not contain any serum protein or other proteins, a large proportion of the lymphocytes will stick to the walls of the mixing vessel. The use of plastic or siliconized vessels does not prevent this.The number of cells which in that way will be eliminated from the suspension is dependent on several variables, such as the type of mixing procedure and mixing vessel, the composition of the dilution fluid, and the cell density. As a consequence it is difficult to give any correction factor for such a count in a haemacytometer counting chamber. More than 30% of the cells may disappear under generally obtained conditions. This source of error is to some extent also involved in the white blood cell count, despite the presence of about 1% protein in the dilution fluid from the serum and lysed erythrocytes.To prevent this loss of cells either bovine serum albumin, to a concentration of 5%, or the detergent Certrimide, to a concentration of 2–5 mM, may be added to the dilution flu

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb02704.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

The total amount of iron absorbed from a total supplementation of 2000 mg of iron was studied after a blood donation. 10 regular blood donors and 10 subjects without previous blood loss were included in the study. No subject had received any iron treatment before the investigation. Both groups were given one sustained release tablet containing 100 mg of iron as Fe SO4once daily for 20 days. The iron in these tablets was labelled with59Fe. The total amount of iron absorbed from the tablets was measured by a whole body counting technique. Laboratory tests including haematological data, desferrioxamine tests and sternal marrow punctures were made before and after the iron treatment. The non‐donors lost a mean amount of 215 mg iron by phlebotomy and the total iron absorption from the tablets was only 94 mg (4.7%). In the regular blood donors the corresponding iron loss was 276 mg and the absorption was 280 mg (14%). The iron supplementation given in this study covered the iron losses in the regular blood donors but not in the non‐donors. This iron prophylaxis will prevent a depletion but not a reduction of the iron stores in blood donors with high donation freque

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb02705.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

The defect in iron (Fe) absorption in X‐linked anaemia (sla) remains an enigma; absorption of a tracer dose of Fe is impaired in mice raised on an iron‐containing cube diet but not in those raised on an iron‐deficient diet. Because cobalt (Co) shares a similar intestinal transport pathway with Fe, a study was made of the effect of iron deficient diet on Co absorption. The duodenum ofslaand genetically normal mice was perfused for 30 min with labelled solutions containing Co or Fe. Co uptake and transfer were similar inslaand normals fed cubes whereas Fe uptake and transfer were less inslathan in normals. The iron deficient diet caused an increase in the uptake and transfer of Co and Fe inslaand normals. When Co and Fe were perfused together inslafed deficient diet, the uptake and transfer of each metal was less than when perfused alone. The distribution of Fe and Co in subcellular mucosal fractions was determined by a differential centrifugation technique. Deficient diet resulted in a directionally similar change in the subcellular dstribution of Co and Fe inslaand normals.The increase in Co as well as Fe absorption in theslaon an iron deficient diet to the same high level found in genetically normal animals, and the inhibitory effect of each metal on the absorption of the other suggests that the absorption defect inslais unlikely to be due to a primary defect in the function of the transport ca

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb02706.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

A patient with Weber‐Christian disease (syn. nodular nonsuppurative panniculitis) is reported. The generalized cellular destruction in this patient resulted in liberation of proteolytic enzymes into the circulation, which led to multiple haemostatic disturbances with haemorrhagic diathesis. The most prominent haemostatic defects were thrombocytopenia with a normal life span of isologous platelets, high levels of AHF‐related antigen, hypofibrinogenaemia with short fibrinogen survival, low levels of Factor XIII (fibrin stabilizing factor = FSF) and increased amounts of fibrin/fibrinogen degradation products (FDP). Proteolytic enzymes, other than thrombin and plasmin which especially degrade Factor XIII and fibrinogen, derived from destroyed cells (probably leukocytes) seem to have been involved in the pathogenesis of the bleeding disorder in this pati

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb02707.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

The in vivo effect of a single i.p. injection of cytosine arabinoside (250 mg/kg body weight) on the rat bone marrow is reported. Cytosine arabinoside (ara‐C), a potent inhibitor of DNA‐synthesis, was seen to produce selective necrosis of fast cycling bone marrow erythroblasts. The depleted erythroid marrow is replenished by stem cells which are members of the lymphoid‐transitional cells compartment. An indirect correlation between the degree of erythroid cell depletion and the extent of marrow lymphocytosis was noted 6–12 h after the administration of ara‐C. At 24–48 h, however, the marrow returned to its normal activity.The rat bone marrow 6–12 h following a single injection of ara‐C is suggested as an experimental model for providing stem cells committed to the

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb02708.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

Ultrastructural observations of erythroid colonies in diffusion chambers implanted in lethally irradiated rats are reported. The donor cells were obtained from rats treated with cytosine arabinoside (250 mg/kg body weight) 6 h prior to seeding. The marrow of donor rats contained a high proportion of stem cells committed to the erythroid line.9 days after implantation, vast numbers of erythroid colonies developed in the diffusion chambers. The erythroid cells were observed arranged around a central macrophage resembling erythroid islands in bone marrow. Erythroid colonies most probably differentiated from committed stem cells in the inoculum.It is postulated that development of erythroid colonies in diffusion chambers is conditioned by, among other factors, the presence of a special type of macrophages. These macrophages have the same function as those of central reticular cells (CRC) in bone marrow erythroid islands.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb02709.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

Aging of rat erythrocytes was studied in vivo by two experimental models. In the first, a single cohort of erythrocytes labelled with radioiron was followed; in the second, sequential hypertransfusions were employed to obtain a defined red cell population, which was continuously transferred from host to host. The results are compatible with a normal red cell life span of about 60 days and with sequestration of senescent erythrocytes in the spleen and in the liver. Red cell aging was associated with a decline in cell volume, haemoglobin, G6PD and ATP content. There were no measureable changes in osmotic fragility.Thus, changes in osmotic resistance do not appear to be critical factors limiting the life span of circulating erythrocytes in the rat.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb02710.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb02711.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY