ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1986.tb00816.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Chronic lymphocytic leukaemia (CLL) is a lymphoproliferative disorder which sometimes also affects the erythrocytes. In this study we investigated erythrocyte involvement in 23 CLL patients. We found increased erythrocyte osmotic fragility in 15 CLL patients, but this finding was not accompanied by increased permeability to acidified glycerol (decreased AGLT50). Only those CLL patient who had positive direct antiglobulin test (DAT) had significantly decreased AGLT50. AGLT cannot serve as a predictor test for the future development of autoimmune haemolytic anaemia in CLL patients. ATP content was unaffected by the lymphoproliferative disease, but whole blood filterability was markedly decreased in CLL patients. Our study supports the hypothesis that erythrocytes are indeed affected by the lymphoproliferative disorder, even in the absence of overt autoimmune haemolytic processes.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1986.tb00817.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

A patient is described who presented a thrombocytopenia with thrombocytopathy followed by the development of a leukaemia. The disorder was characterized by decreased aggregation in the presence of ADP, and a lack of aggregation in the presence of arachidonic acid, natural endoperoxide or collagen. In parallel,14C‐serotonin release was severely decreased or nil in response to these inducers. Thrombin induced a slightly decreased aggregation and a normal14C‐serotonin release. Thromboxane B2(T × B2) synthesis was normal after stimulation by arachidonic acid, natural endoperoxide or thrombin showing a normal arachidonate metabolism. In addition, the mepacrine test showed no significant decrease of the number of dense bodies with an average of 4.6 per platelet (versus 5.4 ± 0.8 sd in controls). Stimulation by ionophore A 23187 failed to induce aggregation,14C‐serotonin release, or T × B2synthesis. Furthermore, in the presence of EDTA, A 23187 did not provoke activation as reflected by14C‐serotonin release or T × B2synthesis. Thus, in this case of thrombocytopathy, the hypothesis of abnormal intracellular Ca+ +fluxes responsible for the defective platelet release phenomenon, wa

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1986.tb00818.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

2 patients with acute megakaryoblastic leukaemia (AMKBL) were successfully treated with a combination of aclarubicin hydrochloride (an anthracyline), enocitabine (a derivative of cytosine arabinoside) and 6‐mercaptopurine (6‐MP) or 6‐thioguanine (6‐TG). They achieved a complete remission following 1 or 2 courses. They remained well and in complete remission throughout 3 courses of consolidation therapy, a total of 9 weeks.The results of remission induction therapy of AMKBL have been reviewed in the literature. 4 of 7 adult patients, including our cases, treated with 3 drugs, anthracycline, cytosine arabinoside or its derivative and 6‐TG or 6‐MP, achieved a complete remission. AMKBL may not have so poor a prognosis as previous

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1986.tb00819.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Immune cells cultured in vitro against autologous blast cells were infused twice in 8 patients with ANLL after achieving complete remission. 3 of the 8 patients remained in first remission for 74–94 months and they appeared to be cured of their leukaemia. These 3 patients reveiced significantly higher numbers of in vitro sensitized immune cells as well as having a better recovery of the in vitro cultured cells. The other 5 patients relapsed within a year of diagnosis; 3 of them had had organomegaly and a preceding myeloproliferative disorder at diagnosis. None of 20 ANLL patients in complete remission treated simultaneously with similar chemotherapy at our institution remained in first complete remission, nor were long‐term survivors. Adoptive immunotherapy with in vitro sensitized immune cells may be effective treatment in acute leukaemia. The therapeutic potential of infusion of in vitro stimulated autologous immune cells requires further investigat

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1986.tb00820.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

3 patients with acute leukaemia, HLA antibodies and thrombocytopenia refractory to random donor platelet transfusions were treated with high‐dose i.v. immunoglobulin. All 3 patients responded favourably with improved post‐transfusion recovery of random platelets. In 1 patient, the recovery of transfused histocompatible platelets was also enhanced. Treatment was followed by reduction in the total lymphocyte count and marked changes in lymphocyte subsets in 1 pati

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1986.tb00821.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

A case of persistent cerebellar toxicity following systemic high dose cytosine arabinoside (HDCA) treatment of acute myelogenous leukaemia is reported. The symptoms, which developed at a cumulative dose of 32 g/m2, subsided to some extent following discontinuation of the drug, but left signs of cerebellar dysfunction 10 months later. A review of previously published reports indicates that the cerebellar toxicity of HDCA, which has been ascribed to loss of Purkinje cells, usually occurs when the accumulated dose exceeds 36 g/m2. That it may occur at even lower doses is supported by the present case. In addition, available evidence of re‐appearance or worsening of previously induced signs of toxicity following its repeated administration stresses the importance of immediate and permanent cessation of cytosine arabinoside therapy, when signs of cerebellar dysfunction develo

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1986.tb00822.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

We investigated the recloning capacity of normal and chronic myeloid leukaemia granulocyte‐macrophage colony‐forming cells (CFU‐GM) after 7 d culture in methyl‐cellulose. Normal CFU‐GM were recloned with the formation of 3.52 ± 1.12 secondary CFU‐GM per primary d‐7 colony. A frequency distribution of the colony‐forming cells within d‐7 colonies showed a heterogeneous distribution with the majority of d‐7 colonies containing 1 or zero CFU‐GM and a few colonies containing more than 30 CFU‐GM. Separation of marrow cells by velocity sedimentation demonstrated that the recloning capacity was primarily expressed by the smallest colony‐forming cells. In contrast, marrow or blood cells from 18 patients with Ph1‐chromosome‐positive CML in the chronic phase produced colonies with defective recloning capacity. Only 1 patient had a recloning value within the normal range; the others had a mean value of 0.35 (range 0–1.28) secondary colonies per primary d‐7 colony. The recloning defect was not related to WBC or treatment, since 5 newly diagnosed patients with CML also showed defective recloning before any treatment was given, which is compatible with the defect

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1986.tb00823.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

We report 3 cases of allogeneic bone marrow transplantation in multiple myeloma that we have recently performed. Following conditioning treatment with i.v. cyclophos‐phamide (60 mg/kg/d, for 2 d), oral melphalan (1.0 mg/kg/d, for 5 d), i.v. BCNU (5.5 mg/kg, in a single dose) and total body irradiation (10 Gy in a single fraction) we observed in all 3 cases the disappearance both of serum M component and of monoclonal bone marrow plasma cells. 1 patient died of acute GVH disease, grade IV, at 2 months, while the other 2 patients are in good health and in unmaintained complete remission at 4 and 20 months, respectively. The usefulness of allogeneic bone marrow transplantation in the management of multiple myeloma is emphasize

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1986.tb00824.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

The effect of peripheral blood mononuclear cells (PBMCs) from bone marrow recipients on in vitro growth of erythroid burst‐forming units (BFU‐E) was studied. PBMCs were obtained from 5 allogeneic, 1 syngeneic and 1 autologous bone marrow recipient(s) at different intervals after transplantation. The number of BFU‐E was significantly increased when donor bone marrow cells were co‐cultured with PBMCs obtained from allogeneic marrow recipients in the early post‐transplant period. No effect was observed using PBMCs obtained in the later post‐transplant period, PBMCs from a syngeneic marrow recipient, or PBMCs from an autologous marrow recipient. The BFU‐E enhancing activity was present among T cells and was abolished by treating them with OKT3 or OKT4 antibody and complement. These observations suggest that chimeric T lymphocytes, probably of the helper/inducer subset, from allogeneic marrow recipients in the early post‐transplant period have a potent enhancing effect on in vitro

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1986.tb00825.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY