摘要:

In the recipients of an allogeneic HLA‐identical sibling transplant the blood leucocytes were reconstituted within 3 to 4 weeks but the level of lymphocytes remained low throughout the observation period of 20 weeks. Of the different lymphoid cell subsets, the large granular lymphocytes (LGL) reconstituted fastest, followed by DR‐expressing lymphocytes. The reconstitution was accompanied by a significant lymphoid blastogenesis in the blood. The frequency of OKT4 and OKT8 lymphocytes was initially low; the number of OKT8‐positive lymphocytes reached normal levels by the 6–8th week whereas the number of OKT4‐positive lymphocytes and, consequently, the OKT4/8 ratio remained low. The responses to the T mitogens, phyto‐haemmagglutinin and concanavalin A, were strongly suppressed. Only a few significant changes were observed before and during acute graft‐versus‐host disease (aGVHD): the frequency of LGL, lymphoid blasts and OKT8‐expressing lymphocytes was depressed before aGVHD and the frequency of lymphoid blasts remained depressed throughout the episode. We conclude that reproducible changes occur in the leucocyte subset frequencies during reconstitution, but the characteristic changes prior to and during aGVHD are not particularly prominent and hardly

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb02385.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

The association of monoclonal gammopathy and lymphoma was investigated in 100 consecutive, untreated cases of chronic lymphocytic leukaemia (CLL) and non‐Hodgkin's lymphoma (NHL) of B‐cell type, classified according to the criteria of Lukes&Collins (9). The overall incidence of monoclonal immunoglobulins (Ig) was 24%. The highest incidence (57%) was seen in plasmacytoid lymphocytic lymphoma and the lowest (7.9%) in cases of CLL. IgM was the predominant class of monoclonal Ig. It is concluded that the presence of monoclonal gammopathy in NHL reflects the stage of differentiation and maturation of the malignant B‐cell

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb02386.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

8 patients with known sickle cell anaemia were studied immunologically. The concentrations of the main immunoglobulin classes, IgG and IgA, were significantly higher than the levels in 11 normal age‐ and sex‐matched black subjects (P<0.01). IgM levels were not significantly different in the two groups. There was a heterogeneity in the interaction of the IgG subclasses with Protein A, with low levels of IgG2. The IgG2:IgG1 ratios varied from 1:3.8 to 1:6 (normals 1:3). In 4 patients the absolute levels of IgG2 as measured by radial immunodiffusion were lower than normal, thus confirming the chromatographic ratios. Since specific antibody is often restricted to a single subclass, the levels of IgG subclasses may be related to recurrent bacterial infections in these patie

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb02387.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Cytogenetic analyses of bone marrow cells were carried out by means of direct technique and short‐term cultures in 25 acute promyelocytic leukaemia (APL) patients. 16 were affected by the hypergranular form and 9 by the M3‐variant. The t(15;17) was documented at diagnosis in 15 patients. In addition, 2 cases, in which an M3morphology and a normal karyotype on direct preparation were found at diagnosis, disclosed the chromosome anomaly in cultured cells at relapse, in concomitance with a change of blast morphology to the M3‐variant. However, the t(15;17) was a consistent feature in 5 of the 15 patients with cells analysed on direct preparations only and in all cases who had successful bone marrow cultures. Overall, the 15;17 translocation was detected in 7 out of 9 patients with the M3‐variant and in 8 out of 16 patients with typical M3. Whenever the chromosome preparations were made after culture, 7/7 had the translocation in the M3‐V group and 5/5 in the M3group. No obvious differences in clinical features or outcome were evident in the patients, irrespective of their cytogenetic

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb02388.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Complete removal of leucocytes and platelets from erythrocytes and the development of a sensitized procedure for the assay of G6PD activity allowed the biochemical mechanisms of the Mediterranean variety of G6PD deficiency to be re‐evaluated. Activity in the young erythrocytes from 9 G6PD‐deficient subjects averaged 0.1% of the levels observed in the corresponding erythrocyte fraction from normal individuals: moreover, the decline of activity during aging of the G6PD‐deficient erythrocytes was comparable with that observed for the normal enzyme. Mutant G6PD purified from granulocytes of a G6PD‐deficient subject and entrapped within the corresponding erythrocytes was remarkably stable. Exposure of native erythrocytes to an oxidative stress (divicine plus ascorbate) resulted in a decrease of G6PD activity that was significantly more rapid and extensive in control than in G6PD‐deficient cells. These results seem to exclude enhanced intracellular breakdown of the mutant protein within the circulating ery

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb02389.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Platelet aggregation was investigated in platelet‐rich plasma from normal volunteers before and at various times after intake of 10 analgesic drugs. The drugs used were aspirin, piroxicam, naproxen, indomethacin, diclofenac, ibuprofen, diflunisal, paracetamol and oxyphenbutazone. Aggregation of the platelets was induced by adrenaline or ADP and the first and second waves of aggregation were evaluated. It was found that no drug exerted any effect on the first wave of aggregation. The second wave of aggregation was abolished by aspirin that produced a long‐lasting effect for 5–8 d. Piroxicam also abolished the second wave of aggregation and this effect persisted on the 2 following d. Naproxen was normalized in half of the volunteers on the 2nd d. The inhibition caused by indomethacin and diclofenac was corrected on the 2nd d. Ibuprofen and diflunisal produced a definite but short‐term effect. The effect of salicylic acid was weak. Paracematol and oxyphenbutazone did not affect platelet aggr

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb02390.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

A consecutive series of patients (1978–1981) comprising all patients with acute leukaemia from a population of 475000 inhabitants was reviewed. Thus, 94 patients were diagnosed as having acute leukaemia. No patients were lost from follow‐up. The incidence figures of ALL and AML differed significantly from those of Sweden as a whole. 9 patients were<15 years old. The median age of adult patients was 64 years, 60.8% being ≥ 60 years old.Of adult patients with AML, 20% had a preleukaemic history (chronic myeloproliferative disorders, myelodysplastic syndromes and others). None of 6 patients with leukaemia as a metamorphosis of a chronic myeloproliferative disorder achieved a complete remission. The overall remission rate of the remaining adult patients was 25%. Treated patients, 15–39 years old, with AML without any preleukaemic history, had a complete remission rate of 80% compared to 12% for patients ≥ 60 years old with the same diagnosis.Of 60 patients with ‘primary’ AML, 14 were not treated, mainly because of advanced age and complicating diseases. Most of these patients died within a week

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb02391.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

A 73‐year‐old man with lung squamous‐cell carcinoma simultaneously developed a factor VIII:C inhibitor and a transient positive direct Coombs' test. The factor VIII:C inhibitor was characterized as a mixture of IgG1, IgG2, IgG4with χ and Λ light chains. The Coombs' test was IgM‐specific.Treatment: blood transfusions (packed red cells), cyclophosphamide and prednisone. Bleeding subsided without factor VIII‐replacement therapy. Before death, in spite of the tumour‐mass progression, factor VIII

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb02392.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Two‐thirds of osteogenesis imperfecta (OI) patients claimed that they bruised easily. We have studied haemostasis in 58 subjects with OI, tarda type. The most frequent abnormalities were increased capillary fragility (35%), decreased platelet retention (33%) and reduced factor VIII R:Ag (23%). Reduced ristocetin cofactor, deficient platelet aggregation induced by collagen and prolonged bleeding time were less common findings. The combination of vascular, platelet related and plasmatic defects may reflect that OI is a heterogenous group of disorders with common clinical expression. The tourniquet test is the most valuable screening test of the haemostatic defects observed in O

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb02393.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Unsaturated plasma transcobalamin II (UTC II) and cobalamin were measured in two selected age‐groups of like‐sexed mono‐ and dizygotic twins. For UTC II, a higher mean level was found in women than in men, and in the older (57 to 61 years) than in the younger (33 to 39 years) age group. Testing of genetic‐environmental models revealed that variation in plasma levels of UTC II is almost exclusively genetically determined. More than 50% of the variation in cobalamin levels was accounted for by genes, the remainder being due to person‐specific environmental factors (for older males no model gave a good fit). A negative correlation was noted between UTC II and cobalamin levels. The correlation coefficient was low, and the variation in UTC II accounted for only about 4% of the variation in the cobalamin level. This finding suggests that a pathologically high value of one of the variables may have clinical significance, regardless of the value of the other variable. For 22 patients studied longitudinally, a clear tendency to maintain plasma levels at constant levels over long periods of time was found, suggesting that certain degrees of deviation from these levels may have clinical

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb02394.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY