摘要:

A 41‐year‐old man developed intense itching without visible cutaneous changes, epigastric pressure pain, and a slight intolerance to alcohol. He was found to have persistent blood eosinophilia.The eosinophil granulocytes were of abnormal appearance in the light microscope: larger than normal, the nuclei were multilobulated (4–6 lobes), the cytoplasm contained atypical, large granules, ample glycogen, and up to 12 vacuoles.In the electron microscope too the eosinophil granules were entirely atypical, having an electron‐dense matrix, often with a light central inclusion body which was inhomogeneous, having longitudinally oriented structures with a periodicity of about 10 nm. These findings are quite contrary to normal eosinophil granules.Enzymic studies of cytoplasmic enzymes from the granulocytes revealed a greatly reduced content of eosinophil cationic proteins, whereas 5 (7) other enzymes were present in a normal or slightly reduced quantity. The phagocytic capacity of the eosinophils against latex particles was normal.The patient developed generalized lymphomas, histologically very malignant, of the convoluted, acid phosphatase positive cell type (T‐cell lymphoma). Sub‐population studies of lymphocytes from a lymph node revealed 58 % TEcells, while the remainder were B cells. At death, 31/2 years after the onset of symptoms, severe endomyocardial fibrosis was found. The thymus could not be identified.It is concluded that lymphomas should be described on the basis of clinical, histological, and histochemical criteria as well as studies of lymphocyte sub‐populations and that the highly unusual eosinophil granulocytes still deserve particular attention. The endocardial fibrosis is assumed to have been due to substances liberated from the eos

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1977.tb02338.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

Human granulocytes release 25–30 % of the granular neutral proteases, collagenase and elastase, to the exterior of the cell during phagocytosis of yeast cells or immune complexes. Similar amounts of myeloperoxidase and lactoferrin are released. Crossed immunoelectrophoresis demonstrated that collagenase and elastase released extracellularly formed complexes with serum α1‐antitrypsin. The presence of α1‐antitrypsin complexes with granulocyte collagenase and elastase were also demonstrated in inflammatory processes, e.g. in the peritoneal exudate of acute peritonitis. The reactivity of neutrophil proteases with natural plasma protease inhibitors must be considered in assessing the role of these proteases as the etiologic agent of tissue damage and degradation during the inflammatory

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1977.tb02339.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

Serum ferritin (SF) is elevated in adults with malignancies, chronic inflammatory disease, liver disease and iron overload. The purpose of this study was to determine whether the concentration of SF in children with a variety of malignancies correlated with the activity of their disease. Patients with acute lymphoblastic leukaemia (ALL) at initial diagnosis (n = 11) and relapse (n = 15) had a mean SF of 238 and 338 ng/ ml, respectively, compared to the normal mean of 31 ng/ml and range of 7 to 140 ng/ml in children. In 30 patients with ALL in remission the mean SF was 109 ng/ml, less than the values in patients with active disease and greater than the normal mean (P<0.001). The concentration of SF was also increased in a group of 77 patients with a variety of solid tumors. The 28 cases with active disease had a mean SF of 242 ng/ml, significantly higher (P<0.001) than the value of 84 ng/ml in 49 patients with no evidence of residual tumor. The differences in SF concentration did reflect the activity of disease in the groups as a whole but it remains uncertain whether the assay will prove useful in following the response to treatment of patients with certain types of tumor.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1977.tb02340.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

A follow‐up study of 10 patients suffering from acquired aplastic anaemia, comprising methacrylate‐embedded bone marrow biopsies and CFU cultures, is presented. The haematopoietic recovery patterns and changes in the inflammatory infiltration after permanent engraftment could be distinguished from those in non‐transplanted patients. After anti‐thymocyte globulin treatment followed by allogeneic bone marrow infusion, the recovery pattern resembled that in non‐transplanted patients. The persistently low colony‐forming capacity in some patients could be explained by the existence of lymphoid inhibitory cells, which suggests an immunologic auto‐destruct

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1977.tb02341.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

Human lymphoid cells were examined by scanning electron microscopy (SEM) to see if a correlation existed between surface morphologic features and the presence of various surface markers and receptors. When viewed by SEM thymocytes appeared as smooth‐surfaced cells with few surface microvilli; peripheral blood lymphocytes (PBL) on the other hand were moderately to densely villate with no entirely smooth‐surfaced cells observed. Surface morphology within PBL samples was not uniform, due mainly to variations in the shape and number of microvilli. However, 2 distinctive types of surface morphology (termed Types 1 and 2) were discernable with a small number of cells displaying features of both groups (Type 3). The majority of E‐rosette forming cells (T lymphocytes) displayed Type 1 and the majority of cells bearing demonstrable surface immunoglobulin (B lymphocytes) displayed Type 2 morphology. Exposure of PBL to anti‐T cell specific ALG resulted in cytolysis of cells with Type 1 morphology while cells with Type 2 morphology appeared largely unaffected. PBL with Fc and C3receptors displayed all 3 types of morphology. It is concluded that T and B lymphocytes do have subtle but nevertheless discernable differences in surface morphology and within these 2 groups, variations in surface morphology are probably associated with changes in the physiological status of t

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1977.tb02342.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

A modification of the screen filtration pressure technique has been developed to allow platelet response to collagen to be measured. Using a commercial collagen suspension this method has been found to be both simple to perform and to give highly reproducible results. Changes in platelet response to collagen after administration of acetylsalicylic acid were clearly detected and this suggests that collagen screen filtration pressure may provide a sensitive method for evaluating the effect of platelet release inhibiting drugs.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1977.tb02343.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

Recycling of radioiron by renal tubular cells was studied in rats labelled by the i.v. injection of free59Fe‐Hb. Within 24 h, most of the renal radioactivity has been transferred from Hb to cellular ferritin and haemosiderin. Between day 1 and 14 of the study, urinary loss of radioactivity was less than 1 % and most of the reduction in renal radioactivity represented transfer of renal iron into the circulation. An unexpected flexibility in the ability of rat kidney to recycle iron into the body has been found ranging from 13 % in hypertransfused rats to 70 % in bled animals. The high rate of renal iron transfer in bled animals was maintained under experimental conditions simulating chronic haemoglobinuria when most of the Hb removed by bleeding has been reinjected in the form of soluble Hb. Thus, epithelial cells in rat kidney as well as in the gut, appear to be able to transport iron with a much greater efficiency than in man, thereby contributing to the supply of iron for the rapid rates of growth and reproduction characteristic of this animal specie

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1977.tb02344.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

A new radioisotope dilution assay for vitamin B12‐intrinsic factor complex is described. The method is based on the use of the binding type intrinsic factor antibody (the binding reagent), which when combined with the intrinsic factor‐vitamin B12complex (labelled ligand), is quantitatively adsorbed onto zirconium phosphate gel at pH 6.25. The new assay has been shown to provide a measure of intrinsic factor comparable with other intrinsic factor assays, but it has the important advantage of being able to measure the unlabelled vitamin B12‐intrinsic factor complex (unlabelled ligand), and will, therefore, be valuable in the study of physiological events in the gastrointestinal tract.During the study, it was found that there is some evidence for at least two types of binding intrinsic factor antibody: One which combines preferentially with the intrinsic factor‐vitamin B12complex and one which combines equally well with this complex or with free intrinsic

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1977.tb02345.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

Comparative study of isocnzymic patterns of acid phosphatase, β‐glucuronidase and α‐naphthyl acetate esterase of normal and chronic lymphocytic leukaemia (CLL) lymphocytes was carried out by means of polyacrylamide gel electrophoresis. The isoenzymograms of acid phosphatase and β‐glucuronidase of leukaemic cells were similar to that of normal lymphocytes. However, different patterns were found in the case of α‐naphthyl acetate esterase. Two prominent anodal bands were present in CLL lymphocytes; these bands were either absent or very weak in n

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1977.tb02346.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

Phagocytosis of small latex particles was demonstrated by light and electron microscopy in a proportion of cells from B‐lymphoproliferative disorders including chronic lymphocytic, prolymphocytic and hairy cell leukaemia. It was not observed in a case of T‐chronic lymphocytic leukaemia or in normal lymphocytes. A significant correlation was observed between the binding of latex to the lymphocyte surface and the degree of phagocytosis. It is suggested that the phagocytic activity of leukaemic B‐lymphocytes is mediated through their Fc receptor and that this property may also be present in normal B‐cells.Among mononuclear cells, therefore, phagocytosis can no longer be considered exclusively a property of mo

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1977.tb02347.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY