ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01170.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

The measurement of59Fe clearance from the plasma is a well established means of investigating erythropoiesis. The effect of variation in plasma iron concentration, contamination by59Fe not bound to transferrin and59Fe counting accuracy on the59Fe clearance curve are described. A valid analysis of the clearance curve cannot be made unless these errors are eliminated. A technique is presented which allows the clearance of [59Fe] transferrin to be studied free of extraneous influences.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01171.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

In previous studies, we observed an increase in intracellular cyclic AMP in platelets during thrombin‐induced nucleotide and calcium release. Our present work suggests that platelet prostaglandins are directly involved in this phenomenon. Washed human platelets were incubated at 37°C with human thrombin for times ranging between 5 s and 5 min. The release of nucleotides and calcium in response to thrombin, determined spectrophotometrically, reached 40–60% of maximal plateau levels by 5 s, 70–80% by 10 s, and 90–100% by 15 s. The production and secretion of prostaglandin E (PGE), measured by radioimmunoassay, parelleled this rapid time course. After 10 s, however, the amount of extracellular PGE began to decrease, continuing to do so through 5 min of incubation. Intracellular cyclic AMP accumulation, determined by radioimmunoassay, lagged behind the time course of nucleotide and calcium release, and that of PGE secretion, by several seconds. This lag in cyclic AMP accumulation appeared to correlate closely with the disappearance of PGE from the supernate in the same platelet samples. In view of the inhibitory effects of cyclic AMP and PGE on platelet release and aggregation, these interrelated time course curves suggest the existence within platelets of a mechanism for the modulation of platelet

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01172.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

The activity of the erythrocyte enzymes: glucose‐6‐phosphate dehydrogenase, pyruvate kinase, glutathion reductase and ATPase were measured in 8 patients with untreated myelomatosis. Glucose‐6‐phosphate dehydrogenase was significantly increased. Glucose‐6‐phosphate dehydrogenase values were negatively correlated with the glomerular filtration rate as measured by51Cr‐EDTA clearance. The results support the existence of a shortened red cell survival in peripheral blood related to the degree of renal

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01173.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

Bone marrow chromosomes were studied in a series of 50 consecutive, unselected cases of untreated polycythaemia vera (PV). A pathological cell line was present in 7 patients (= 14%), 3 patients each had 1 extra chromosome no. 8 and 1 extra no. 9. 2 patients had a similar but not identical marker chromosome including material from the long arms of chromosome no. 1. 1 of these patients also had a 20q – deletion. 1 patient had an extra unidentified isochromosome. 1 patient had a monosomy 16. In 5 cases the abnormal clone dominated over cells with a normal karyotype. In each of 6 patients a singular hyperdiploid cell was observed. 1 or several polyploid or randomly hypodiploid cells were found in most patients.The findings in our material are compared to other reports of chromosome studies in PV and allied disorders. Except for the 20q – deletion no abnormality typical for PV has so far been detected, but some chromosomes seem to be involved more often than others (1, 8, 9, 20). Patients with chromosome aberrations did not show any clinical, laboratory or morphologic features which could separate them from cases with normal karyotypes. The prognostic significance of the chromosome abnormalities encountered in early PV cannot be defined at pres

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01174.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

Bone marrow chromosomes were studied in 2 patients with polycythaemia vera (PV) at the time of diagnosis and several times subsequently during therapy with chlorambucil. Both patients were normal in the initial study, but later developed pathological diploid cell lines with structural chromosome abnormalities. Case 1 presented after 25 months of myelosuppressive therapy a deletion of chromosome no. 12, del (12) (p11), in 60% of the diploid cells. 10 months later all studied cells in the marrow contained this deletion. In addition, the deletion of chromosome no. 20, described as characteristic for PV, was found in 3 cells. Case 2 demonstrated after 40 months on chlorambucil the presence of a balanced translocation, t (1; 5) (p36; q31) in 90% of the cells. In none of the patients were any definite clinical, laboratory or morphological signs of an approaching leukaemic transformation of the polycythaemic disorder present.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01175.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

The relationship between platelet function and plasma free fatty acid concentration has been studied serially during the initial 24 h in 11 patients suffering from acute myocardial infarction and in 5 patients with severe angina pectoris. Similar results were obtained in the 2 groups. Plasma free fatty acid concentration was initially high, and decreased significantly. The distribution of plasma free fatty acids remained unchanged. Platelet concentration was constant, whereas the percentage of reversible venous platelet aggregates initially was higher than in 11 healthy persons matched for age and sex. Platelet aggregates decreased transiently at 16 h. Venous reversible platelet aggregates correlated significantly with concentration of plasma free fatty acid, thus establishing a possible link between a change in lipid metabolism and platelet function. Plasma concentration of platelet factor 4 increased slightly but significantly during the initial hours. This may indicate an increased platelet release reaction.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01176.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

5 healthy male volunteers received i.v. infusions of isoprenaline in doses of 0.03, 0.06 and 0.09 μg × kg−1× min−1over a period of 6 min. A statistically significant decrease in the peripheral platelet count was obtained, but no dose‐response‐curve for isoprenaline was observed. It is suggested that while an i.v. infusion of epinephrine causes a release of platelets from the exchangeable splenic platelet pool, isoprenaline might have the opposite effect on the spleen, thereby causing a decrease of platelets in the periph

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01177.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

The effect of 10 days of total fasting (energy deprivation) on blood polymorphonuclear granulocyte functions, leukocyte numbers, iron and transferrin levels was evaluated in 14 healthy, normal‐weight males. Granulocytes from 7 of the subjects were tested in vitro. A statistically significant depression was noted in their bactericidal capacity against Staph. aureus. The 14 subjects showed a marked decrease in the stainable activity of granulocyte alkaline phosphatase and decreases were noted in plasma iron and serum transferrin levels. The iron saturation of serum transferrin was unchanged. Thus, impairment of granulocyte bactericidal functions may occur secondarily to short‐term total energy deprivation, in the absence of iron deficie

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01178.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

A 42‐year‐old male patient became hospitalized with severe back pain and marked bleeding tendency from disseminated intravascular coagulation. The bone marrow aspirate showed numerous nests of cancer cells presumably from a prostatic carcinoma. After only 4 days of treatment with diethylstilbestrol his condition was markedly improved, and a new bone marrow aspirate showed extensive necrosis of the cancer ce

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1976.tb01179.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY