摘要:

2 children with cyclic neutropenia were treated with lithium carbonate. In one patient, fever and stomatitis were ameliorated, but the duration of neutropenia was increased and parotitis occurred. In another patient, aphthous stomatitis disappeared but fever persisted. The duration of neutropenia was prolonged and submaxillaritis and sinusitis occurred.Thus, lithium administration may lead to complications when the duration of neutropenia is prolonged. We recommend discontinuation of the drug, even in the presence of amelioration of symptoms.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1983.tb00640.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

The activity of dipeptidylaminopeptidase IV (DAP IV) was investigated by a cytochemical method in isolated fractions of normal peripheral blood mononuclear cells and malignant cells from 9 patients with chronic T‐cell leukaemia. A positive DAP IV reaction was observed in 52 % of normal T cells, a consistent negative reaction in normal B cells and monocytes. 2 distinct T‐cell subsets, helper/inducer cells (T4+/T8‐) and cytotoxic/suppressor cells (T4‐/T8+), were negatively selected by complement‐mediated cytolysis utilizing the monoclonal antibodies OKT4 and OKT8. On examination of these T‐cell subsets a positive DAP IV reaction was restricted to the majority of normal T4+/T8‐cells. The malignant cells from 3 patients with T‐chronic lymphocytic leukaemia, expressing the cytotoxic/suppressor pheno‐type (T4‐/T8+) lacked DAP IV activities. In contrast, almost all leukaemic cells from 3 other cases, expressing the helper/inducer phenotype (T4+/T8‐), were strongly positive. Despite their T4+/T8‐phenotype, the malignant cells from 3 patients with Sezary syndrome were completely DAP IV negative. These findings suggest that the DAP IV reaction may be helpful in the further characterization of normal and m

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1983.tb00641.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Platelet turnover, platelet production, platelet mean life span (MLS), platelet count, mean platelet volume (MPV) and platelet‐associated antibodies have been examined in 26 patients with chronic idiopathic thrombocytopenic purpura (ITP) and in 1 patient with hypomega‐karyocytic thrombocytopenia (HT). 15 ITP patients had normal or increased platelet turnover and platelet production, while 11 had subnormal values despite shortened MLS, while the patient with HT had normal MLS. The differences between the two groups with high and low platelet turnover were statistically significant. No correlation was found between kinetics parameters and bone marrow pattern in a total of 19 patients examined. These data suggest that in some cases of chronic ITP, the pathogenesis of thrombocytopenia can be due not only to the peripheral destruction of platelets, but also to a deficient platelet production by megakaryocytes. Since the number of megakaryocytes in bone marrow slides is not decreased in the low turnover compared with the high turnover group, it is possible that an impaired pattern of megakaryocyte maturation be the cause of the low platelet production in these patients, unlike in the HT patient where megakaryocytes are almost abs

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1983.tb00642.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Pyruvate kinase (PK) and phosphofructokinase (PFK) erythrocyte deficiencies induced by chemotherapy were studied in 6 patients. From immunological tests it may be assumed that the PK and PFK deficiencies were due to different direct mechanisms: a disturbance of the synthesis of one of the PFK subunit; mutation(s) in the structural gene of PK which result in the synthesis of mutant proteins. Several molecular mechanisms are probably at the origin of all the disturbances induced by chemotherapy in the red blood cells. The study of these alterations which mimic those detected in preleukaemic and leukaemic states, provides information on the molecular events in the possible production of chemo‐induced cancer

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1983.tb00643.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

The distribution within the body of autologous leukaemic cells labelled with111In oxine was studied in 4 patients with blast crisis of chronic myelocytic leukaemia (CML) by means of serial samples and gamma camera imaging. Leukaemic cells of the blood initially entered the spleen and liver, and the major site of localization was the former rather than the latter. A portion of leukaemic cells, which rapidly entered the liver, left temporarily 3 h after reinjection. Leukaemic cells entered the spleen maximally at 3 or 24 h and then continued to leave gradually or rapidly up to 48 h. The majority of leukaemic cells in acute myelocytic leukaemia did not leave the spleen for up to 48 h. It is suggested that the destruction of sinusoidal structures of the spleen due to marked infiltration of leukaemic cells in CML impairs the effects of filtration in endothelial cells of sinusoids and facilitates the entry of leukaemic cells into sinusoids from splenic cords.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1983.tb00644.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

The radiosensitivity of human cord blood haematopoietic stem cells was measured by assessing colony formation in methylcellulose cultures and diffusion chambers. The results show that fetal colony forming units, granulocytic, monocytic (CFU‐GM), are radiosensitive: colonies formed by electron‐irradiated CFU‐GM, both in spontaneous and in colony stimulating factor (CSF) supplemented cultures decrease in number in an exponential fashion with increasing doses of irradiation. D0 values up to 800 rads were, for highly purified fetal lymphoid cells, 160 ± 88 rads; for whole buffy coat cells, 155 ± 75 rads; and for buffy coat cells plus exogenous CSF: 155 ± 77 rads. Extrapolation numbers (N) approached the value of 1. Among cord blood diffusion chambers progenitor cells (DCPC), radiosensitive and radioresistant subpopulations of myelopoietic stem cells exist, as tested over a range up to 1500 rads. Monocytopoietic CFU‐D are radi

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1983.tb00645.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

The lactoferrin content of human plasma has been measured by an enzyme‐linked immunosorbent assay. In cord blood the level was 0.02‐0.3 mg/l, corresponding to 3–44 × 10‐10mol/l lactoferrin; in plasma 5 d post partum the level had not changed. In adults the level was 0.02‐0.2 in 29 out of 30 plasma samples and above 1 mg/l in 1 sample. Similar results were obtained with EDTA, citrate or heparin as anti

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1983.tb00646.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

A study of 35 patients with elevated Hb and haematocrit but normal or borderline red cell mass has confirmed that the most common disease association was hypertension and that the peripheral blood findings are reversible when blood pressure is treated, especially when the body haematocrit is low. The situation is probably the result of abnormal distribution of red cells in the circulation. The red cell pool in these patients is normal and they show no evidence of progression to polycythaemia vera. Some cases are associated with smoking and obesity. It is more rational to treat the underlying disease than to attempt to alter the blood volume by venesection.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1983.tb00647.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

4 months after a bone marrow transplantation performed for acute lymphocytic leukaemia, a 28‐year‐old man had encephalitis. A brain CT scan revealed bilateral and symmetrical temporal hypodense areas. Serological studies revealed a recent CMV conversion in serum and a higher conversion in cerebral spinal fluid. All other viral antibodies remained at low levels, especially for herpes simplex virus. Because of a high incidence of CMV infection after bone marrow transplantation, the responsability of CMV as the cause of the encephalitis is discus

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1983.tb00648.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

To determine whether clonal karyotype abnormalities seen in myeloproliferative disorders originate in cells of only one or of more blood cell lines, committed erythroid and granulocyte‐monocyte progenitor cells were cultured in vitro by the methyl cellulose assay, and chromosome studies were carried out on the colonies. 3 patients were studied, 1 with polycythaemia vera and 2 with myelofibrosis, which was preceded by polycythaemia vera in 1 of the cases. All analysable karyotypes from both erythroid and granulocyte‐monocyte colonies in every patient showed the clonal karyotype aberration which had been demonstrated in the bone marrow or blood of the patient. This finding is evidence of the involvement of a multipotent stem cell in the clonal proliferation of these disea

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1983.tb00649.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY