摘要:

Peripheral blood lymphocytes from 48 consecutive, newly diagnosed CLL patients were examined by light‐ and electron‐microscopy for the presence of nuclear cleaving or folding. On this basis, the patients could be subdivided into 2 easily separable morphological groups. In 42 patients the lymphocytes had regular round nuclei. In 6 patients (13 %) the lymphocytes of peripheral blood had a highly irregular nuclear outline characterized by pronounced cleaving or folding of the nuclear membrane. In 4 of these 6 patients immunological investigations demonstrated membrane markers consistent with B‐cell monoclonality. Age, sex, distribution between stages as well as mode of clinical presentation were all alike in the 2 groups of patients. In spite of this a statistically significant, highly increased mortality was observed in patients with lymphocyte nuclear irregularities. Thus, 50% of all deaths occurred in this small group of patients.These findings indicate that lymphocyte nuclear cleaving or folding represents a stage‐independent prognostic parameter, which permits selection of a small but easily identified high‐risk group of

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1983.tb00628.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

In a retrospective survey of 334 cases of non‐Hodgkin lymphoma (NHL), 250 cases could be classified according to both Rappaport's classification and the Kiel classification. All patients initially in stage I had an excellent prognosis irrespective of histology, whereas patients in stages II, III and IV had a worse prognosis that did not significantly differ between stages. Rappaport's classification could separate stages II‐IV into 2 different prognostic groups, one favourable and one unfavourable group. According to the Kiel classification, 3 prognostic groups ‐ favourable, intermediate and unfavourable ‐ were found. The survival rate for favourable Rappaport fell in between favourable‐Kiel and intermediate‐Kiel. Both subgroups within favourable‐Rappaport (nodular lymphomas and diffuse well differentiated lymphomas) could actually be subdivided by the Kiel classification into one group with a more truly favourable behaviour and one with an intermediate behaviour. The extent of follicularity among nodular lymphomas and the presence of plasmacytoid differentiation among the small lymphocytic lymphomas were found to be of prognostic importance. In conclusion, we found that the Kiel classification separated the more indolent lymphomas better than Rappaport's classification. No preference was found concerning the high gr

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1983.tb00629.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

A quantitative assessment of total, effective and ineffective erythropoiesis, and mean red cell life‐span was performed in 2 patients with idiopathic cold‐haemagglutinin disease (CHAD) who had severe anaemia, reticulocytopenia and erythroid bone marrow. In both cases, ineffective erythropoiesis represented the major factor in the pathogenesis of the anaemia. The most likely explanation of this was that cold antibodies had some effect on the maturing red‐cell precursors. Ineffective erythropoiesis has already been shown in patients affected by auto‐immune haemolytic anaemia due to warm antibodies with reticulocytopenia and erythroid bone marrow. Therefore, it is apparent that both warm and cold antibodies may cause not only peripheral destruction of mature red cells but also intramedullary death of red‐cell p

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1983.tb00630.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

In a prospective study, the hypothesis of whether the quantitative determination of platelet‐associated IgG (PAIgG) in patients with chronic autoimmune thrombocytopenic purpura (ATP) can predict the efficacy of splenectomy, was investigated. PAIgG levels were repeatedly determined in 16 patients with definite ATP pre‐ and postsplenectomy, and related to platelet counts and platelet mean life span. It was found that patients with an immediate remission after splenectomy tended to have lower PAIgG levels (<6%) than failures, but this difference was not statistically significant. We conclude that PAIgG is of limited value for the prediction of the efficacy of splenectomy in

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1983.tb00631.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

A myeloproliferative condition in blastic phase is described in an 18‐year‐old male who was also found to have a mediastinal malignant teratoma. Myeloid metaplasia was found in the lymph nodes and spleen, and an infiltration of granulocytic blast cells was observed in the bone marrow and the lymph nodes. Aneuploidy with an extra chromosome (trisomy 8) was present in bone marrow cells. To our knowledge the combination of a myeloproliferative disorder and a malignant teratoma has not been earlier descri

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1983.tb00632.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

In a 64‐year‐old female with chronic lymphocytic leukaemia, nearly 50 % of the lymphocytes presented one or several globular intracytoplasmic inclusions resembling Russell bodies which were characterized by immunofluorescence as IgM lambda. On electron microscopy, the inclusions looked like cisternae of the rough endoplasmic reticulum. Surface membrane Ig was not detected and the serum IgM was low. The histology of a lymph node showed a lymphocytic proliferation with evidence of Ig synthesis, compatible with immunocytoma. The aggressive course of the disease necessitated polychemotherapy. This case represents an unusual ill‐defined lymphoproliferative disorder, lying between the B‐CLL and a non‐secretory macroglob

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1983.tb00633.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

33 patients with chronic myeloproliferative disease were investigated for megakaryocytes in cubital venous blood. 6 Patients had chronic myeloid leukaemia, 11 polycythaemia vera and 16 myelofibrosis. In all patients, a significantly higher number of megakaryocytes than in normal adult humans was found. In patients with chronic myeloid leukaemia and myelofibrosis, an increased number of intact megakaryocytes was found, indicating thrombopoiesis in the peripheral part of the upper extremity. 2 patients with myelofibrosis showed a % of intact megakaryocytes>25, as found in blood from the inferior vena cava in normal adults. In patients with chronic myeloid leukaemia, some of the megakaryocytes were smaller than normal, whereas in myelofibrosis, some of the megakaryocytes were larger than normal, with increased lobulation of nuclei. Active thrombopoiesis in the peripheral part of the extremities can be of great importance, when splenectomy is considered in treatment of myelofibrosis.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1983.tb00634.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Cell marker analysis including mouse red cell roesetting ability was carried out on 24 newly diagnosed patients with chronic lymphatic leukaemia. Using a technique not employing neuraminidase a significant difference in mouse red cell rosetting ability was noted between male and female patients. It is well accepted that females often have a milder form of disease and better longterm survival than it is suggested this might be a reflection of the difference found in mouse red cell rosetting ability between the sexes in this condition.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1983.tb00635.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Blood and lymph node T‐lymphocyte subpopulations have been studied in untreated non‐Hodgkin lymphoma (NHL) patients and healthy controls. T‐lymphocytes were determined by the E‐rosette technique and by OKT3/LEU4 monoclonal antibodies; OKT4/LEU3 and OKT8/LEU2 monoclonal antibodies were used to identify T‐cell subsets with helper/inducer and suppressor/cytotoxic activity, respectively. OKT4+T‐cells were low in patients, while OKT8+T‐cell numbers were normal. The OKT4+/OKT8+blood lymphocyte ratio was below the normal range in about 50% of the patients. The ratio was higher in lymph nodes than in blood of patients and controls. The results may suggest that untreated NHL patients have a reduced pool of T‐cells with phenotypic markers of OKT4/LEU3. Monoclonal blood B‐lymphocytes were found in 45% of the cases. The presence of such cells in blood was frequently associated with a low

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1983.tb00636.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

The surface glycoprotein patterns of leukaemic B lymphocytes from 20 patients with clinically progressive or non‐progressive chronic lymphocytic leukaemia (CLL) were investigated. Cells were labelled by the neuraminidase‐galactose oxidase‐tritiated sodium borohydride technique and the radioactive proteins were separated by polyacrylamide slab gel electrophoresis and visualized by fluorography. A total of 13 to 16 bands were detected. A common surface glycoprotein pattern for CLL cells was seen in all patients consisting of 7 proteins with the apparent molecular weights of 210, 200, 185, 150, 135, 110 and 90 kilodaltons, respectively. Interesting differences were, however, observed as cells from patients with progressive CLL in general lacked the glycoproteins 120, 72 and 67 K, which were found on cells from inactive CLL. The possible biological and clinical significance of these findings is disc

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1983.tb00637.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY