摘要:

The usefulness of the epinephrine stimulation test in detecting granulocytic hypersplenism is evaluated. In 8 hypersplenic patients, an increment of the granulocyte count of 170 % over baseline values was found, compared to 38 % (P<0.001) in normal subjects and 44 % (P<0.002) in patients neutropenic from other causes. No overlap of individual test results was found among the hypersplenic and the control groups. Epinephrine test discriminates between neutropenia from hypersplenism and neutropenia from other causes. Furthermore, this test is found to be a valuable adjunct to the hydrocortisone stimulation test in kinetic evaluation of neutropenia from any cause.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1981.tb00477.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

A human haemopoietic cell line (K562) which exhibits various erythroid characteristics, has been utilised as a model system for studying erythropoietic differentiation. We have analysed the alterations in expression of cell membrane determinants which accompany the induction of Hb synthesis. We confirm that the K562 cell line exhibits a number of erythroid features: it expresses immunologically detectable membrane proteins, glycophorin A and spectrin and can be induced, by addition of haemin or n‐butyrate, to synthesise Hb. N and i‐like blood group activities are demonstrable in uninduced K562, but band 3, ABH and other major alloantigens characteristic of mature erythrocytes are lacking. The acquisition of cell surface antigens typifying erythroid or other haemopoietic lineages has not been demonstrated following induction, although certain properties not associated with morphologically recognisable red cell precursors are lost. The relevance of these findings to the foetal nature and possible multipotentiality of the K562 cell line are discus

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1981.tb00478.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Phenotypic changes in blast crisis of a case of Philadelphia chromosome (Ph1)‐positive chronic myelogenous leukaemia were characterized by serial terminal transferase (TdT) determinations simultaneously related to cytochemical and cytogenetic data. At the onset of the blast crisis, 90% of the blast cells were acid phosphatase‐positive (focal pattern), Ph1‐positive, lymphoid cells. The TdT activity amounted to 29 units/108mononuclear cells in the peripheral blood and to 57 units/108mononuclear cells in the bone marrow. Therapy with vincristine and prednisone caused the elimination of the TdT‐positive cell population. 4 months later, there was an increase in TdT‐negative, myeloid blasts which was brought under control with busulfan. Cytogenetic analysis of the myeloid blasts still revealed Ph1positivity in 100 % of the metaphases examined and the lack of additional chromosomal abnormalities. A second relapse was again dominated by TdT‐containing cells with the 46,XX,Ph1karyotype. This time, the patient failed to achieve remission with vincristine and prednisone. Even though the TdT activity was markedly decreased, the lymphoid blast count remained elevated and the cells showed resistance to further therapy. This failure of morphology, cytochemistry as well as cytogenetics to distinguish between the individual phenotypes emerging during the course of blast crisis of CML characterized the TdT as a cell marker of important diagnostic and therapeutically progn

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1981.tb00479.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Ferritin estimations were performed in serum from 38 Ethiopian and 10 Swedish pregnant women and in cord blood from their newborn infants. The mean ferritin level in the Ethiopian mothers was significantly higher than in the Swedish mothers as well as in a non‐pregnant population of apparently healthy Swedish women. The Ethiopian mothers consisted of two groups with different economic situations, a privileged and a non‐privileged group. The non‐privileged group consumed the iron‐rich traditional diet unique for Ethiopia, whereas some of the privileged mothers had abandoned this menu in favour of more refined food items with a lower iron content. Subnormal ferritin levels, indicating iron depletion, were found in 33 % of the privileged and in 8 % of the non‐privileged group, in spite of the fact that the infants of the privileged group had a significantly higher mean birth weight, indicating a better nutritional standard in general. It is concluded that the previously reported rarity of gestational anaemia in Ethiopia is due mainly to the good iron state of Ethiopian women, especially those who still eat the traditional cereal bread. No correlation was found between maternal and cord blood ferritin. The relevance of cord blood ferritin in the diagnosis of the iron state is

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1981.tb00480.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Bleeding is a common complication in patients suffering from multiple myeloma. In some cases a defect in fibrin formation has been suggested as one possible cause of haemorrhagic tendency. As shown in this investigation the defect in fibrin formation, ascertained using PAGE, is due to a lack of α‐chain polymerization of fibrin monomers in 5/11 patients with IgG myeloma and in 2/5 patients with IgM paraproteinaemia. No disturbed fibrin polymerization could be observed in IgA myeloma (n = 6). Factor XIII concentrations of subunit A and to a lesser extent of subunit S (Laurell technique) were highly elevated in all cases with regular fibrin formation. Comparable values were obtained by measuring the transamidase activity of factor XIII by incorporation of14C‐labelled purtrescin into casein. Levels up to 600% of normal could be recorded. In contrast, all patients with a lack of α‐chain polymerization had a factor XIII activity within the normal range. Addition of factor XIII concentrate to plasma from patients with defective fibrin formation led in 5/8 cases to a partial cross‐linking of α‐monomers. We conclude that in some cases paraproteins can inhibit the factor XIII and prevent its acti

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1981.tb00481.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Giant lymph node hyperplasia is a lesion of lymphoid tissue, which may be accompanied by systemic manifestations like fever, anaemia and hyperglobulinaemia. A case is described with temporal arteritis, an association hitherto not described in the literature.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1981.tb00482.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Rats were maintained in an atmosphere of equal volumes of oxygen/nitrous oxide (1/1) for up to 7 d and plasma levels of methionine, glycine, serine, histidine, homocysteine and S‐methylcysteine were measured. There was a fall in plasma methionine and a rise in plasma serine levels. There were no significant changes in glycine and histidine levels. Homocysteine and S‐methylcysteine were not detected in rat plasmas. The fall in plasma methionine was due to loss of cobalamin‐dependent methionine synthetase activity. The rise in plasma serine may be due to decline in its metabolism via methenyltetrahydrofolate cyclohydrolase which is concerned in oxidizing the methenyl‐carbon (=CH–), initially derived as a methylene‐carbon (–CH2–) from serine, to

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1981.tb00483.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Antibody‐dependent cell‐mediated cytotoxicity (ADCC) by monocytes was determined in 45 adults approximately 4 years after splenectomy for either abdominal trauma or accidental injury during operation for benign gastroduodenal ulcer. Compared to controls, both groups of splenectomized persons showed increased numbers of monocytes and decreased monocyte ADCC. The decrease of monocyte ADCC was only significant in those splenectomized persons in whom accessory splenic tissue was not detectable by isotope scanning. Addition of the spleen‐dependent peptide tuftsin to monocytes in vitro did not augment their ADCC. An increased serum concentration of immune complexes was demonstrated in 18 % of the splenectomized persons, but this increase was neither releated to the presence of splenic tissue nor to the monocyte cytotoxicity. Vaccination with pneumococcal polysaccharide had no effect on monocyte ADCC.It is suggested that the defective monocyte cytotoxicity observed in splenectomized persons without detectable splenic tissue might be relevant to post splenectomy infectious susceptib

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1981.tb00484.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

102 patients with chronic lymphocytic leukaemia underwent clinical staging according to 2 systems: the Rai staging system with 5 stages (0: lymphocytosis, I: palpable lymph nodes, II: hepato‐ or splenomegaly, III: Hb below 110 g/1, IV: platelet count below 100 times 109/1) was compared to a staging system recently proposed by Binet, with only 3 stages (A: not more than 2 areas of palpable nodes or organs, B: at least 3 areas, C: Hb below 10 g/1 or platelet count below 100 times 199/1). With the 3‐stage system statistically significant differences between the stages were obtained, whereas some of the Rai stages (I‐II‐III) were only poorly separated. Non‐haemolytic anaemia with Hb below 100 g/1 was associated with a far graver prognosis than milder anaemia (Hb between 100 and 110 g/1) or auto‐immune haemolytic anaemia. Chest X‐ray findings did not influence the c

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1981.tb00485.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

20 patients with benign monoclonal gammopathy (BMG) have been studied for blood lymphocyte and subpopulations. 4 patients had slightly decreased T‐lymphocyte values. Total B‐lymphocytes were within the normal range. In 3 BMG patients an abnormal ratio between χ‐ and Λ‐bearing lymphocytes was detected indicating circulating monoclonal cells. 41 patients with untreated multiple myeloma have also been analysed for blood monoclonal lymphoid cells using the χ:Λ‐ratio. 54% of the patients had monoclonal blood cells at diagnosis. The incidence and numbers of such cells increased with advanced clinical stage. Thus, it seems as if tumor volume is the main factor responsible for the appearance of monoclonal blood cells in mul

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1981.tb00486.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY