摘要:

Erythrophagocytosis in human bone marrow has been studied in selected series of iliacal bone biopsies embedded in methacrylate or epoxy resin. Using established light microscopic criteria for recognition of the early digestive erythrophagic vacuole, intense erythrophagic activity was encountered in a series of 5 patients with haemolytic disease. A series of 32 biopsies, selected to represent ‘normal’ bone marrow, demonstrated low or modest erythrophagic activity in all cases. The findings are in accordance with previous demonstration of erythrophagocytosis in human bone marrow at autopsy and point to the bone marrow as the main site for destruction of aged red cells in the healthy human organ

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb00311.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb00312.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

The occurrence of transient extreme neutrophilic leucocytosis, with white blood cell counts exceeding 100,000/μl in two adult patients with myeloproliferative diseases, polycythaemia vera and acute myelocytic leukaemia in remission, is reported. The reaction was transient, and the subsequent course of the patient's disease did not differ from that prior to the episode. Such extreme leucocytosis is rare in adults, despite the frequency of provoking conditions, and factors which predispose an individual to develop a leukaemoid reaction remain unknown. An increase in the size of the pool of proliferating granulocyte precursors accompanied by a loss of the restraining function of the marrow sinusoidal walls in myeloproliferative diseases may explain the augmented release of myeloid cells in our patients

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb00313.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

Cyanate (NCO), which impedes sickling by increasing the oxygen affinity of sickle haemoglobin, may react with many proteins in the body and potentially interfere with many functional systems. Leukocyte glycogen was studied because elevated liver glycogen has been noted after high dose NCO treatment in rats. Leukocyte function was also studied in peritoneal exudates.In animals given NCO alone, blood leukocyte glycogen was 2.8 ± 0.3 (SE) mg per 109WBC while control values were 3.3 ± 0.6, a difference not statistically significant. In casein‐induced peritoneal exudates, more WBC were recovered from NCO‐treated animals (265 times 106WBC vs. 214 times 106WBC; P = 0.0009). Glycogen in blood leukocytes of casein‐stimulated animals was not significantly different from NCO‐treated animals.Leukocyte glycogen in peritoneal exudates was markedly increased over blood leukocyte glycogen in both controls (19.4 ± 0.6 vs. 2.7 ± 0.3 mg per 109/WBC; P<0.0001) and NCO‐treated animals (17.5 ± 0.7 vs. 3.6 ± 0.5 mg per 109WBC; P<0.001), although levels in exudates from control and NCO‐treated animals did not significantly differ from each other.14C‐glucose incorporation into glycogen was significantly increased (P = 0.020) in exudate leukocytes of NCO‐treated animals. Initial phagocytic rates were equal in exudate leukocytes of control and NCO‐treated animals.Although NCO treatment has demonstrable effects on some aspects of leukocyte glycogen metabolism, exudation of leukocytes and phagocytic fu

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb00314.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

This study is intended to clarify whether or not platelets are essential to endotoxin‐induced precipitation of soluble fibrin representing the second phase of microclot formation. Renal glomerular microclots could be observed in extremely thrombocytopenic rabbits to the same extent as in normal rabbits after the injection of endotoxin into animals with circulating soluble fibrin. Thrombocytopenia was induced by feeding rabbits busulphan, and soluble fibrin was produced by infusing ancrod. The experiments indicate that platelets are not essential to the endotoxin‐induced precipitation of soluble fib

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb00315.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

A single dose of prednisone (2 mg/kg orally) resulted in a mean rise in neutrophil counts to 2.3 (range 1.5–3.6) and a mean decrease in plasma lysozyme to 0.73 (range 0.50–0.87) times the value before the steroid was given in 10 haematologically normal persons. There was no change in the intraneutrophilic lysozyme activity.It is concluded that the decrease in plasma lysozyme is compatible with a reduction in the egress of neutrophils to the tissues and a decrease in the neutrophil turnover r

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb00316.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

Comparative microspectropholometry of the DNA content of Feulgen‐stained megakaryocytes from the bone marrow and from those retained in the pulmonary circulation showed that the ploidy classes 8 N, 16 N and 32 N are found in both bone marrow and pulmonary circulation, whereas the 64 N class is found in the bone marrow only.Electron microscopic examination of megakaryocytes retained in the lungs revealed typical ‘naked nucl

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb00317.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

Cytogenetic studies have been carried out on bone marrow aspirates from 25 patients with myelomatosis. Abnormal stem lines were present in 7 of the patients; the remainder had a diploid chromosome complement. In most patients — also in those without an abnormal clone — some metaphases had a blurred appearance similar to that seen in bone marrow aspirates from patients with acute leukaemia. In many of the chromosome preparations obtained before cytostatic therapy some metaphases with structural aberrations of the chromosomes were seen. Evidence is presented that in the patients with abnormal stem lines in the bone marrow, the chromosome abnormalities are confined to the myeloma cells and are not found in the erythrocytic or granulocytic precursors, which thus do not seem to be involved by the neoplastic process. Based on the present results and on a review of the relevant literature some general cytogenetic features are emphasized which may contribute to a better understanding of the disorder. Especially, it is demonstrated that in myelomatosis the cytogenetic changes are much more uniform than in other malignant disorders with the exception of chronic myeloid leukae

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb00318.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

10 patients thymectomized about 20 years earlier were tested for delayed skin reactivity against mumps, streptokinase‐streptodornase and PPD antigens. Their T and B lymphocytes in peripheral blood were determined. 6 patients were surgically thymectomized and 2 of them received postoperative irradiation. 4 patients were treated with only high dose irradiation to the mediastinal compartment. None of the patients responded to all skin antigens, 1 was positive to two antigens and 3 to one antigen. The remaining patients were non‐responders. The number of peripheral blood lymphocytes was slightly decreased in the patients. A significant decrease in the number of T‐lymphocytes (p<0.005) was found in the whole patient group, and the depression was more pronounced in the surgically thymectomized patients (p<0.001). The number of B‐lymphocytes was unaltered. It is concluded that the abnormalities in this patient group reflect a T‐lymphocyte deficiency. The immunologic status of the patients before treatment is not known. However, in 3 patients with recently discovered untreated thymomas no abnormalities were found. Thus, adult thymectomy in man seem to lead to deficiency of cell mediated immunity, indicating an influence of the thymus in immunological functions during a

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb00319.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

A previous report have shown that both intact red blood cells (RBC) and an equal number of haemolyzed cells were able to inhibit erythropoiesis on ESF‐induced erythropoiesis, suggesting a cell compound to be responsible for the inhibitory effect. Haeme and haeme compounds have been found to stimulate or inhibit both erythropoiesis and haeme synthesis. The present work presents data on the inhibitory effect of haemolyzed RBC and compounds from intact RBC on erythropoiesis. The inhibiting factor was found to be of a small molecular size and of the same range as a urinary erythropoiesis inhibiting factor (EIF). The inhibitor did not contain haeme. Both Fe2+and Fe3+were tested, showing no reduction of the59Fe incorporation into RBC in the test animals. The inhibition could not be due to dilution of the59Fe with unlabelled iron from the haemolyzed cells. On the contrary, Fe3+‐ions rather stimulated erythropoiesis, probably due to increased amounts of available iron. Haemolysates were prepared from RBC with different amounts of immature cells. With increasing amounts of reticulocytes, a reduction of the inhibitory effect occurred. Also foetal cells showed less inhibition than an equal amount of adult cells. After high speed centrifugation, the inhibitory effect of haemolysates was found in the supernatant, while ghost cells exerted no inhibition. No species differences were found using both exhypoxic polycythaemic mice and rats. An inhibiting factor was liberated into the incubation medium when RBC were incubated for 20 h. No haemolysis occurred during the incubation period. Mature, adult RBC therefore contain a substance which is different from haeme, with a negative feedback on erythropoie

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1975.tb00320.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY