摘要:

A 40‐year‐old woman splenectomized 17 years previously for hereditary haemolytic anaemia was investigated in our laboratory because of persistent conjunctival subic‐terus associated with compensated haemolysis. The results of the autohaemolysis and osmotic fragility tests were similar to those usually observed in hereditary spherocytosis. Red cell enzyme assays indicated a decreased amount of kinetically normal enolase. The genetic transmission of this defect could not be established since the only other affected member of the family was the proposita's father who died several years ago after splenectomy for an undefined haemolytic dis

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb00716.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

11 patients with chronic neutropenia were evaluated. In 6 patients low numbers of CFU‐DG were observed. Only 3 of these patients also had low CFU‐C numbers, suggesting that these two precursors are not identical. Bone marrow samples from 2 patients were restudied a year later. The number of CFU‐DG remained low while CFU‐C numbers increased to control range in 1 patient. Studies for serum inhibitors were negative. No cytogenetic abnormalities were observed.This study suggests that abnormalities at different levels in the haemopoietic precursor cell hierarchy can be detected either simultaneously or independently in patients with chronic neut

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb00717.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Antithrombin III (AT‐III) deficiency may be due to quantitative or qualitative AT‐III reduction. The diagnosis of qualitative disorder is suspected when a discrepancy is found between immunological and functional levels of AT‐III. Heterogeneity has been hypothesized in both quantitative and qualitative deficiency of AT‐III. A technique based on crossed immunoelectrofocusing (CIEF) was applied to investigate molecular variants of AT‐III. 3 families with low functional and immunological levels of AT‐III and 1 family with only a low functional AT‐III level were investigated. An abnormal AT‐III pattern was found with CIEF in the family with suspected qualitative disorder and in 1 of the families with quantitative disorder. The 2 abnormal patterns were different. Thus the use of CIEF AT‐III patterns could help to define congenital AT‐III deficiencies and could serve as a basis for classification of different forms o

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb00718.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Liver and bone‐marrow of 20 human foetuses between 15th and 20th week of gestational age were examined. Mononuclear cells were labelled with murine monoclonal antibodies to reveal surface antigens and with a rabbit antiserum to TdT for the detection of the nuclear enzyme, using a double colour indirect immunofluorescence technique. The results have revealed that TdT+cells express the phenotype of B cell precursors HLA‐DR+, CALLA+, FMC8+, LEU‐1‐, LEU‐5‐, LEU‐9‐‐. It suggests that foetal liver and bone‐marrow have an active function in B‐lymphopoie‐sis in humans. The involvement of TdT in the process of B‐cell immunological acquisition is uncertain because only humans and Xenopus embryos, among the species examined, express TdT outside the

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb00719.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Selected functions (i.e. phagocytosis and chemotaxis of circulating blood monocytes were studied on cells obtained from 20 patients with untreated multiple myeloma (MM) and the results were compared with those obtained on cells from 60 healthy persons. The mean number of circulating monocytes was only slightly increased over the normal value although a marked monocytosis was demonstrated in all of the 4 patients with M‐components of the lambda light chain type. Plasma cells isolated from peripheral blood and from bone marrow of patients with MM were found to possess a strong a‐naphthyl acetate esterase activity. No differences were observed in the phagocytic activity of monocytes from patients compared with those of the controls, whereas the chemotactic responsiveness of the monocytes from the patients was slightly increa

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb00720.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Ferritin was extracted from human hepatocellular carcinoma tissue and purified using column chromatography, gradient gel electrophoresis and cadmium sulphate crystallization. DEAE cellulose chromatography showed a difference between hepatoma and normal liver ferritin, indicative of a more acidic isoferritin profile in the tumour. Column‐purified and crystalline ferritin and that remaining in the mother‐liquor after crystallization was subjected to isoelectric focusing. Hepatoma ferritin showed higher concentrations of acidic isoferritins than liver ferrtin. This was most obvious with mother‐liquor ferritin, as crystallization tended to select out more basic isoferritins. Subunit analysis of hepatoma and liver ferritin showed a higher proportion of heavy subunits in the tumour ferritin, in keeping with the presence of acidic isoferritins. An antibody against hepatoma mother‐liquor ferritin was raised in rabbits. However, hepatoma ferritin proved to be antigenically identical with normal liver ferritin, and we were thus unable to develop a specific radioimmunoassay for hepatoma f

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb00721.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

The alterations of surface morphology of human platelets during the course of the aggregometer tracing were studied by scanning electron microscopy (SEM). Prior to activation the platelet rich plasma was pre‐incubated at 37°C for at least 30 min in order to obtain a sufficient number of discoid platelets. Immediately following the addition of collagen or ADP platelets showed slender pseudopods which thereafter were replaced by bulbous protrusions. The decrease in percent light transmission (%T) in the aggregometer tracing was characterized in SEM by a significant enlargement of bulbous protrusions and by the transformation of platelet shape from disc to sphere. During the increase in%T platelets forming primary aggregates displayed spiny pseudopods at their surface indicating that, during in vitro aggregation induced by collagen or ADP, two generations of pseudopods are formed. Using a low dose of ADP, the return of aggregometer tracing to the base line was regularly accompanied by dissociation of primary aggregates, but platelets remained spheroid and displayed pseudopods for a long time. Our study indicates that the course of aggregometer tracing is closely associated to the surface morphology of platelets. Single morphological changes, however, are not reflected by the aggregometer meth

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb00722.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

We report a case of Hodgkin's disease associated with Epstein‐Barr virus (EBV) infection in a 75‐year‐old male. The patient was given a 5‐day course of acyclovir to which he responded well, with partial resolution of the lymphoma and return to well‐being. A biopsy from the lymphoma after acyclovir treatment showed a change in the histological picture with a reduction in the number of giant cells. Relationships between EBV infection and Hodgkin's lymphoma are

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb00723.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

12 patients with symptomatic chronic myelofibrosis were treated with either busul‐phan or 6‐thioguanine. Therapy was without significant side effects and resulted in a reduction in the size of liver and spleen and an improvement in well‐being in all cases. In 7 patients the Hb value rose and the extent of marrow fibrosis was reduced in the 5 patients in whom bone marrow examination was repeated after treatment. Chemotherapy is an effective and safer alternative to splenectomy in patients with myelofibrosis with symptomatic anaemia, symptomatic splenomegaly or constitutional symptoms such as fever, weight loss and night s

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb00724.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb00725.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY