摘要:

Increases in plasma concentrations of VIII:C, VIII:CAg, VIIIR:Ag and plasminogen activator (PA) were observed in 50 healthy volunteers given i.v. injections of DDAVP (desaminocys1‐8‐D‐arg‐vasopressin). The PA activity reached its maximum immediately after the injection, VIII:C, VIII:CAg and VIIIR:Ag after 3040 min. However, a positive correlation was found when the PA and VIII:C responses in each of the normals were analysed.DDAVP was also administered to 3 patients with severe von Willebrand's disease. 2 of the patients displayed no changes in VIII:C, VIII:CAg, VIIIR:Ag or VIIIR:RCF and there was no increase in PA. The third patient responded with an increase in VIII:C and to a minor degree in VIII:CAg. This patient developed fibrinolytic activity, but in the lower normal range.In 3 other patients with mild von Willebrand's disease DDAVP caused increases in VIII:C, VIII:CAg, VIIIR:Ag and PA.We feel that the combined data may support the concept that one and the same target cell is involved in the DDAVP mediated release of factor VIII related activities

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1980.tb02749.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

2 patients with pure red cell anaemia (PRCA) have been treated with antilymphocytic globulin (ALG). An 18‐year‐old man with PRCA refractory to corticosteroides and cyclaphosphamide was treated with 16 g of ALG and obtained a complete remission. 3 years later, he was still in complete remission. A 46‐year‐old man with PRCA appearing 6 months following thymectomy was treated with ALG in his third relapse of PRCA. ALG alone had no significant therapeutic effect, but a combination of corticosteroides and ALG induced a remission lasting for 6 months. A second remission was induced when ALG and corticosteroide again were ins

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1980.tb02750.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

α1‐acid glycoprotein (α1‐acid GP) isolated from human plasma was found to inhibit thrombin‐induced aggregation of washed human platelets (final thrombin concentration 0.05 NIH U/ml), and inhibition was complete with physiological concentrations of the glycoprotein (1.0–1.5 g/l final conc.). The inhibitory effect seemed to occur immediately on thrombin addition, thus being similar to the effect of heparin previously observed. As opposed to heparin, however, α1‐acid GP did not affect spontaneous platelet aggregation. Furthermore, α1‐acid GP (in optimal concentrations) reduced the combined inhibitory effect of heparin and antithrombin III on thrombin‐induced platelet aggregation, thus being consistent with the previous findings using heparin thrombin clotting time.Snyder&Coodley (1976) found α1‐acid GP to inhibit platelet aggregation induced by epinephrine and adenosine diphosphate in platelet‐rich plasma. As we also found α1‐acid GP to inhibit collagen‐induced platelet aggregation, this acid glycoprotein may possibly act as an inhibitor of the release reaction although fairly high concentrations (10 mg/ml final conc.) were nee

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1980.tb02751.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

A 30‐year‐old man with clinical and laboratory features of thrombotic thrombocytopenic purpura (ITP) underwent exchange transfusion with 9 units of whole blood over a 24‐h period. Then, the neurological, renal, and haematological manifestations resolved and the patient recovered completely. Serial plasma samples were obtained before and at 8 h intervals during the exchange transfusion. A convalescent plasma sample was obtained 14 and 80 d later. Immune complexes were demonstrated in the pre‐exchange transfusion plasma, by polyethylene glycol (PEG) gradient sedimentation, separated by electrophoresis and analysed immunochemically. There were shown to be light, medium and high molecular weight sedimentation zones in the PEG gradients. The medium zone consisted of immune complexes containing platelet membrane asrociated antigens and IgG antibody; the heavy and light zones only showed the presence of IgG. There was progressive clearance of these zones by the exchange transfusion with the convalescent specimens showing a marked decrease in all zones, especially those of the middle molecular weight (which on analysis represent true immune complexes). The clearance of these immune complexes from the circulation by exchange transfusion could explain the clinical response seen in the

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1980.tb02752.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

The proliferative and differentiating capacity was compared between immature normal marrow cells and marrow, blood or spleen cells from patients with chronic myeloid leukaemia (CML) in chronic and blastic phases. Low density cells highly enriched in myeloblasts and promyelocytes were cultured in diffusion chambers (DC) and implanted into the peritoneal cavity of mice pretreasted with cyclophosphamide. In CML of chronic phase cell production was higher than normal with the exception of spleen cells. Total cell production was lower in blastic phase than in CML chronic phase. The [3H]‐TdR labelling index of myeloblasts of blastic phase CML increased considerably upon implantation in DC consistent with re‐entry into active proliferation. Immature normal cells give rise mostly to macrophages while the corresponding CML cells of chronic phase give a much higher PMN production with peaks after 12–18 d. Also in CML of blastic crisis the blasts differentiate into mature PMNs, but the maturation time is shortened with peaks of PMNs after 5–10 d. DC cultures of spleen cells from patients with CML showed a less differentiating capacity with a very low PMN production compared to cultures of marrow or blood cells. Our results indicate intrinsic differences in growth and maturation capacity between normal immature granulopoietic cells and cells from patients with CML of chronic phase or blastic crisis. The results may also indicate intrinsic differences between spleen, blood or marrow cells

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1980.tb02753.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Immunological methods have been developed for quantitation of a protein, provisionally called L1, which constitutes about 5% of the total protein in granulocytes. The protein has a mol wt of about 51000 daltons and an isoelectric point of about 6.3–6.5. It is readily released from leucocytes during storage and following immunological injury to the cell membrane. The concentration of L1 in normal plasma is about 300 ng/ml, but levels above the normal mean + 5 SD were found in 23 out of 30 patients with various malignant diseases and in 4 patients with septicaemia. It is suggested that plasma levels of L1 may reflect the rate of leucocyte turnover in vivo and that the L1 release phenomenon may be used for studies on leucocyte reactions in vitr

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1980.tb02754.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Mononuclear cells were allowed to adhere to plastic surfaces at 37°C. Lowering of the temperature was followed by detachment of the adherent cells, and recovery as well as purity of monocytes reached a maximum at 4°C. After 10 min at 4°C most of the cells had detached and after 30 min no further detachment could be observed. In this way a mean recovery of 69% of the monocytes was achieved. 18% of the monocytes originally present in the crude mononuclear cell suspension were lost in the non‐adherent cell fraction and the remaining 13% were still adherent at 4°C. Judged from esterase staining 93% of the cells in the detached suspensions were monocytes. The mitogen responsiveness of cells in these suspensions parallelled the number of lymphocytes present. The purity of monocytes in the final cell preparation was inversely proportional to the number of lymphocytes originally present in the mononuclear cell suspen

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1980.tb02755.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Among mononuclear cells isolated from human blood monocytes exclusively mediated antibody‐dependent cytolysis of human type B erythrocytes. Adherent monocytes detached from plastic surfaces by lowering the temperature to 4°C expressed the same cytotoxicity as monocytes contained in the unseparated mononuclear cell fractions. No difference was observed in the cytotoxicity of non‐adherent monocytes and of monocytes remaining adherent at 4°C and subsequently mechanically removed. Removal of carbonyl‐iron phagocytosing monocytes reduced the number of latex‐phagocytosing monocytes without influencing cytotoxicity of the remaining monocytes. In vitro cultivation for 24h of unseparated mononuclear cells and of purified monocyte suspensions increased the number of latex‐positive cells, leaving the number of esterase‐positive cells and the cytotoxic

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1980.tb02756.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Bone marrows from 23 patients with haemopoietic dysplasia were cultured in agar‐gel and the patients were followed for at least 6 months after culture. The incidence of colonies, microclusters and macroclusters and the colony to cluster and microcluster to macrocluster ratios were compared to those of a control group. In the control patients a normal wide range of colonies and clusters was observed and colony growth correlated with cluster growth. About half of the dysplastic patients showed a normal growth pattern and ran a stable and benign course. The other dysplastic patients had defective colony growth, a low colony to cluster ratio, an increased microcluster to macrocluster ratio and a deficient macrocluster growth early in culture. These patients more often presented with cytopenia and had a malignant course with increasing bone marrow dysplasia and a frequent need of blood transfusions. Some patients died of cytopenia or transformed into acute leukaemia.The bone marrow cultures of haemopoietic dysplasia did not predict the short term (within 6 months) risk of leukaemia transformation. However, the results indicate that examination of culture growth can be used to differentiate stable and benign patients from those in whom clonal abnormalities may suggest a progressive and malignant cours

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1980.tb02757.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

From Jan, 1970, to Dec, 1978, 86 previously untreated adolescents and adults with acute lymphoblastic leukaemia were treated in the University Hospitals at Leiden and Nijmegen. Remissions were induced with vincristine and prednisone. If necessary an anthracyclin derivate was added. 73 Patients (85%) achieved a complete remission. CNS leukaemia prophylaxis was given to 64 patients. Two different schedules were used. 35 patients received cranial irradiation with concomitantly intrathecal injections of methotrexate (I+M) after remission had been achieved, and 29 intrathecal methotrexate (M) alone given peridiocally during the remission induction and maintenance treatment. No CNS leukaemia prophylaxis was given to 9 patients (O). Relapses were confined to the CNS alone in 5 cases (group I+M: 1 case; group M: 1 case; group O: 3 cases), to the bone marrow in 40 cases, and to the bone marrow and CNS concurrently in 5 cases (group I+M: 4 cases; group M: no cases; group O: 1 case). A second remission was obtained in 28 patients (56%). The majority of these patients did not receive further CNS prophylaxis. A second relapse of the leukaemia was seen in the majority (23 patients). CNS involvement was found in 6 of these 23 patients. Prophylactic treatment of the CNS in adult acute lymphoblastic leukaemia is mandatory. In our group of patients both schedules of prophylaxis appeared equally effective in preventing CNS leukaemia.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1980.tb02758.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY