ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb02220.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

A case of T cell chronic lymphocytic leukaemia (CLL) with red cell hypoplasia and neutropenia is reported. WBC was 10.0 times 109/1 with 78% being T lymphocytes. These T lymphocytes were positive for Fcγ receptor and had OKT8 and Leu 2a antigens on the cell surfaces. They suppressed both erythroid and granulocytemacrophage colony formation in normal bone marrow cultures and suppressed immunoglobulin production by normal B lymphocytes in vitro. Though the myeloid cells were preserved in the bone marrow and the values of serum immunoglobulins were within normal limits, it can be estimated from these results that red cell hypoplasia and neutropenia were partially due to the suppression of haemopoietic precursor cells by leukaemic T lymphocytes

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb02221.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Factor VIII inhibitors from 4 haemophiliacs have been studied at intervals following transfusion of a factor VIII preparation with an aim to correlate their reaction kinetic and avidity with their immuno‐chemical character. Post‐treatment antibodies are more avid than pre‐treatment antibodies and show a second order reaction while pre‐treatment antibodies produce a graph of complex nature for the time course inactivation of Factor VIII. It seems that IgG3anti VIII:C that forms a significant part of post‐treatment antibody is responsible for these disc

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb02222.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Circulating granulocyte/macrophage progenitor cells (CFU‐GM) were assayed serially during remission in 17 patients with acute leukaemia (9 ALL, 8 AML). In patients with ALL receiving cyclophosphamide, 6‐mercaptopurine and methotrexate, CFU‐GM numbers were significantly lower than in normal individuals; cycles of ‘reinduction’ chemotherapy (vincristine, prednisolone) caused a 10‐fold increase in CFU‐GM per ml of blood. In 2 ALL patients a substantial increase in CFU‐GM numbers preceded the morphologically detectable relapse. In patients with AML receiving repeated courses of cytosine‐arabinoside and 6‐mercaptopurine, circulating CFU‐GM were likewise reduced. In 6 patients who relapsed, a further reduction of CFU‐GM was seen. A complete absence of circulating CFU‐GM was observed in 10 of the 23 investigations performed within 6 weeks prior to the morphologically detectable relapse, while such a ‘zero’‐growth ooccurred in only 1 of 54 experiments performed during stable remission. In summary, in patients with ALL in remission, circulating CFU‐GM are increased following treatment with vincristine and prednisolone. In patients with AML, declining numbers of circulating CFU

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb02223.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Increased iron storage represents a characteristic condition in patients with β‐thalassaemia intermedia. Iron overload is an important factor in cellular damage. Recent studies have shown an enhanced lysosomal fragility due to increased iron storage. 13 patients with β°‐thalassaemia intermedia, aged 17–44 years, were studied. Both serum ferritin and serum N‐acetyl‐β‐D‐glusosaminidase levels were evaluated in all subjects studied. A significant linear correlation (P<0.05) between serum ferritin and serum N‐acetyl‐β‐D‐glucosa

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb02224.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

8 children with acute myelogenous leukaemia were brought into remission with a induction regimen containing cytosin arabinoside (Ara‐C), 6‐thioguanine and adriamycin given as the DNA complex. After remissions were obtained, the children were consolidated with high‐dose Ara‐C (2 g/m2every 12 h for 6 doses) and were given 50000 IU of vitamin A daily as an inducer of differentiation. All children are in continuous relapse‐free survival for periods of 5 to 29 months (mean 1

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb02225.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Serum ferritin concentrations were measured in 909 urban Danish schoolchildren (451 boys, 458 girls) 6 to 17 years old. The children had been randomly selected and served as an urban reference population. Geometric mean ferritin values were identical in boys and girls 6 to 15 years old, while 16‐ and 17‐year‐old boys had higher values than girls (P<0.01). There was a slight rise in mean ferritin values from 6 to 11 years, followed by a temporary fall during the subsequent adolescent growth period. Geometric mean ferritin values and significant differences were as follows: Children 6 to 11 years old (n= 335), 29 μg/l and adolescents 12 to 15 years old (n= 417), 26 μg/l (P<0.01): Adolescent boys 16 to 17 years old (n= 76), 32 μg/l and adolescent girls same age (n= 81), 24 μg/l (P<0.01). The frequencies of low ferritin values60 μg/l (i.e. large iron reserves) were observed in 6.2% of boys and 6.

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb02226.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Immunoprecipitation was performed with platelet‐specific alloantisera (anti‐Zwa, ‐Zwb, ‐Bakaand antiserum Luc) and125I‐labelled platelets of a panel of donors typed for these platelet‐specific alloantigens. This was done by sensitization of intact, radiolabelled platelets with the antisera, solubilization of the sensitized platelets with Nonidet P‐40 and recovery of the immune complexes from the lysate with heat‐killed, formalin‐fixed Staphylococcus aureus, strain Cowan I. In the case of platelet antibodies of the IgM class, bacteria also preincubated with rabbit‐IgG anti‐human IgM before treatment of the lysate were applied. Radiolabelled antigens were then eluted from the bacteria by boiling in an SDS‐mercaptoethanol‐containing buffer. Subsequently, the eluted antigens were analyzed by SDS‐polyacrylamide gel electrophoresis. It revealed that both the Zwaand the Bakaantigens are located on the glycoprotein‐IIb/IIIa complex. The location of the Zwbantigen could not be established in this way.From the intensity of the precipitated glycoprotein bands, it appeared that Zwais probably a marker of glycoprotein IIIa, Bakaor glycoprotein IIb. However, immuno‐precipitates of anti‐Zwaand anti‐Bakasera both carried, to some extent, complexes of glycoproteins IIb and IIIa. Iodinated platelets of a Glanzmann thrombasthenia patient failed to show any material precipitated by these antisera or the antiserum Luc, known to contain an

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb02227.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

The binding of125I‐lactoferrin to a variety of peripheral blood cells was examined. In the concentration range from 10−9mol/l to 10−6mol/l a receptor‐like binding of lactoferrin was observed in monocytes as well as in polymorphonuclear leucocytes. At the low concentrations of lactoferrin in plasma (about 10−9mol/l) the cellular binding to monocytes was about 10 times higher than the binding to polymorphonuclear leucocytes and lymphocytes. A major result from a kinetic analysis was a lower apparent binding affinity to polymorphonuclear leucocytes (KDabout 2 times 10−7mol/l) than to lymphocytes and monocytes (KDabout 2 times 10−8mol/l). Studies in leukaemic cells showed that lymphocytes from patients with chronic lymphocytic leukaemia bound lactoferrin to the same small extent as normal lymphocytes. In contrast, a larger component of binding with high affinity (KDabout 2 times 10−8mol/l) could be demonstrated to lymphoblasts as well as

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb02228.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

We report on a Moroccan family in which the proposita displays a picture of β‐thalassaemia intermedia, associated with heterozygous Hb O‐Arab (β121 Glu → Lys) and a β°‐thalassaemia trait. Hb O‐Arab was ascertained by the disappearance of the Eco RI restriction site that, normally overlaps the β‐globin gene codon 121. The proposita further presents high proportions of Hb F (12.1%) and of Gγchains (68.6%). The transmission of the proband's haemoglobin markers was analyzed (the proband's husband displaying normal haemoglobin). The β°‐thalassaemia andO‐Arabgenes underwent mutual exclusion. A high Hb F (9.28%) level was found in one child, in association with the β°‐thalassaemia trait, while another child carrying the latter trait displayed normal levels of Hb F. This situation suggests that a hetero‐cellular HPFH determinant is involved. However, there was no means to establish whether the high Hb F proportion in the mother results solely from the β°‐thalassae‐mia – Hb O‐Arab association or whether an additional HPFH determinant is present. No DNA deletion was detectably associated with the high proportion of Hb F. In this family, theGγpercentage was high whenever the β°‐thalassaemia gene was present, regardless of total Hb F percentage. This observation is consistent with the view that the control of theGγ per

ISSN:0036-553X    

DOI:10.1111/j.1600-0609.1984.tb02229.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY