摘要:

SUMMARYIn kidney sections treated with the tannic acid‐phosphomolybdic acid‐Levanol fast cyanine 5RN procedure, myoid fibrils were observed in tubular and glomerular cells. The myoid fibrils in tubular epithelial cells were delicate in normal kidneys, but became conspicuous under pathological conditions. Under certain conditions myoid fibrils were found in capsular epithelium and mesangial cells of glomeruli; in some cases podocytes also contained prominent myoid fibrils. There seemed to be a continuous system of myoid fibrils from the media of the afferent arteriole into the mesangium and through the capsular into the tubular epithelium. These myoid fibrils could not be identified with certainty in sections treated with conventional staining methods.Correlations of light‐microscopic observations with electron‐microscopic data indicate that the myoid fibrils in renal epithelial cells visualized by the TP‐Levanol fast cyanine 5RN stain correspond to the myofilamentous system described in electron‐microscopic studies by Pease (1968a). The myoid fibrils in tubular and capsular epithelial cells resembled the long forgotten ‘Basalreifen’ described around the turn of the century. A review of the literature shows that structures corresponding to the basal myoid fibrils were known to early histologists and that contractile functions had

ISSN:0022-2720    

DOI:10.1111/j.1365-2818.1970.tb02209.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

SUMMARYA method for specific, cytochemical staining of cystine residues was applied to thin sections of human hair keratin, two yeasts, one fungus and one green alga, for observations in the electron microscope. The method, which deposits granules of metallic silver at or close to the site of cystine residues, may be a useful means of investigating the detailed distribution of disulphide bonds in cells provided that the following criteria are rigorously applied. First, grains of metallic silver must remain after exhaustive sodium thiosulphate extraction; second, the deposition of grains of metallic silver must be inhibited by prior alkylation of the disulphide linkage. Finally, the method should be used in conjunction with other available means of analysis.

ISSN:0022-2720    

DOI:10.1111/j.1365-2818.1970.tb02210.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

SUMMARYSynaptic vesicles from rat pineal nerves can be stained by Champy‐Maillet mixtures containing osmium tetroxide and a soluble iodide. Two components of the vesicle (the matrix and the dense core) are revealed in a different way depending on at least two factors: the iodide employed and the final pH of the mixture. We have tried the following mixtures: zinc iodide at pH 5·5 (ZIO), cadmium iodide at pH 6·0 (CIO) and potassium iodide at pH 7·2 (KIO‐7) or pH 5·5 (KIO‐5). The matrix is more reactive with ZIO, while the dense core is more reactive with CIO and especially with KIO‐7. With the only difference of a lower pH, KIO‐5 stains the whole matrix of some vesicles and also reveals some tubular structures which are more frequently found in pret

ISSN:0022-2720    

DOI:10.1111/j.1365-2818.1970.tb02211.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

SUMMARYAn apparatus is described which facilitates the sectioning of unfixed and unembedded biological tissues for electron microscopy. The specimen is maintained at the temperature of boiling liquid nitrogen; the knife temperature may be controlled between −50 and − 150°C. Under the worst operating conditions specimen drift with respect to the knife is about 5 nm/sec, and suggestions are made on how this drift may be further reduced. We propose to call the apparatus an ‘ultracryotome’.Electron micrographs of a variety of unfixed, unembedded and unstained tissues are shown and commented on. Some developments of experimental electron microscopy which may be expected to develop from use of the ultracryotome ar

ISSN:0022-2720    

DOI:10.1111/j.1365-2818.1970.tb02212.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

SUMMARYA method for demonstrating collagen and reticulin fibres and the network of brain capillaries has been worked out on the basis of a new silver staining principle. It may be applied to sections as thick as 200 μm from both frozen and embedded material. The principle is as follows: the 1,2‐glycol groups of tissue elements, including connective tissues, are converted to aldehyde groups by periodic acid. The aldehyde groups form colloidal silver particles in an ammoniated silver nitrate solution of pH 13. At this pH value, however, it is only the connective tissue that can bind the colloidal silver which is being formed. After the silver ions have been washed out of the sections, the silver grains deposited in the connective tissue are finally enlarged to microscopic dimensions by physical developme

ISSN:0022-2720    

DOI:10.1111/j.1365-2818.1970.tb02213.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

SUMMARYThe conditions of use of the X‐ray projection microscope for the investigation of bone and cartilage sections have been examined. For the non‐calcified tissues an aluminium target operating at 6 kV has been found the most satisfactory. Aluminium at 15 kV and a copper target at 6 kV are also useful. The instrument is not so satisfactory as conventional microradiography for examination of fully calcified bone, but provides useful information about the softer tissues.In the X‐ray projection microscope a beam of X‐rays produced as nearly as possible from a point is used as a source of illumination, and objects to be photographed are placed between, but not in contact with, the source of X‐rays and the photographic plate. All parts of the specimen or section are equally in focus, so that details from one surface to the other are recorded on the plate with equal sharpness. Because of this depth of focus stereoscopic photographs can be taken to give additional information.The X‐ray microscope is capable of giving useful information where density differences between tissue components are of importance. The resolving power is governed by the size of the X‐ray source, while another factor that influences resolution is the X‐ray scattering factor of the tissue components. The microscope is designed to give X‐rays of different wavelength by change of target material, and of different penetrating power by altering the voltage. At the time this work started, there was insufficient information available about the conditions required for obtaining useful photographs of longitudinal and cross‐sections of bone, so that the first need was to investigate the effect of variations in the experimental method. These will be considered under three main headings: the instrument, preparation and photography of specimens, target material and voltage. An attempt will also be made to give a preliminary assessment of the areas of investigation where the instrument is likely to prove of greatest u

ISSN:0022-2720    

DOI:10.1111/j.1365-2818.1970.tb02214.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

SUMMARYMicroscopical examination of cells, particularly spermatozoa, often necessitates the preservation of a temperature of 37°C on the stage itself. A microscope warm stage should provide a surface at this temperature and allow microscopical examination of a series of pre‐warmed sample slides. A further desirable quality would be the total absence of ancillary apparatus. An apparatus has been designed and built which effectively provides these features. It measures 10·5 × 6·5 × 1·3 cm, weighs 320 g with wiring and plug and provides a thermostatically controlled temperature of 37 ± 0·5°C 5 min after connection with the electrical supply (2

ISSN:0022-2720    

DOI:10.1111/j.1365-2818.1970.tb02215.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

SUMMARYAn apparatus is described for use in time‐lapse cinemicrography. The modifications made to the standard shutter mechanism of the Bolex camera offer increased reliability when it is used in a single‐frame manner over a long period. A reliable method of firing microflash tubes in synchrony with a short shutter‐opening period is also desc

ISSN:0022-2720    

DOI:10.1111/j.1365-2818.1970.tb02216.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

ISSN:0022-2720    

DOI:10.1111/j.1365-2818.1970.tb02217.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

ISSN:0022-2720    

DOI:10.1111/j.1365-2818.1970.tb02218.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY