摘要:

SUMMARY1. The efficacy of streptokinase as an intracoronary thrombolytic agent is wellrecognized. The effect of streptokinase, distinct from its thrombolytic action, on ischaemic myocardium distal to an area of coronary artery occlusion when reperfusion occurs has not been well‐defined.2. In order to do this, myocardial creatine kinase depletion and the histopathology of infarctions produced in rabbits after 1 h of circumflex coronary artery occlusion and mechanical release of the occlusion were assessed.3. Streptokinase or saline was infused intravenously for 1 h beginning 0.5 h after occlusion. Rabbits were divided into two time intervals: early (<10 h) and late (24 h) after release of coronary artery occlusion.4. When streptokinase was infused in early infarctions, haemorrhage did not correlate with infarction cross‐sectional area or myocardial creatine kinase depletion. However, myocardial creatine kinase depletion was 40% less when streptokinase was infused than when saline was infused, suggesting that streptokinase might limit infarct size.5. In late infarctions, the degree of haemorrhage, infarction cross‐sectional area, and myocardial creatine kinase depletion were similar after reperfusion with streptokinase or saline. By 24 h, the beneficial effect of a single dose of streptokinase given early in the course of occlusion‐reperfusion myocardial injury was no longer evident in limiting infar

ISSN:0305-1870    

DOI:10.1111/j.1440-1681.1986.tb02392.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARY1. The nuclear localization of unoccupied oestrogen receptors has been demonstrated in MCF 7 (human breast tumour) cells by immunocytochemistry, and in GH3(rat pituitary tumour) cells by enucleation techniques.2. The present study shows, by similar enucleation techniques, that high affinity (Type 1) aldosterone binding sites are similarly located in the nucleus of steroidunexposed pituitary GH3cells.3. It is, therefore, suggested that such high‐affinity Type 1 sites, which are mineralocorticoid receptors in the kidney and glucocorticoid receptors in the hippocampus, are nuclear‐associated proteins in the absence of steroid and are found in the cytosol compartment only upon cell disrupt

ISSN:0305-1870    

DOI:10.1111/j.1440-1681.1986.tb02393.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARY1. Streptozotocin (STZ)‐diabetes was induced in spontaneously hypertensive (SHR) and Wistar Kyoto (WKY) rats with their litter mates serving as controls. The animals were studied for 6 months and blood pressure, weight, urinary and serum glucose, creatinine clearance, total proteinuria and albuminuria were measured monthly.2. With induction of diabetes, there was a significant rise in creatinine clearance in the hypertensive diabetic animals (SHR‐STZ). SHR‐STZ (n= 6) developed higher levels of total proteinuria than WKY‐STZ (n= 5) although the rise from basal levels was only apparent after 20 weeks of diabetes. All SHR‐STZ developed albustix positive proteinuria after 6 months of diabetes.3. In the first 12 weeks after onset of diabetes, albuminuria increased to a greater degree in SHR‐STZ than in WKY‐STZ. This occurred before there was a detectable rise in total proteinuria.4. The SHR‐STZ model of genetic hypertension and diabetes may be suitable for the evaluation of antihypertensive therapy in human diabeti

ISSN:0305-1870    

DOI:10.1111/j.1440-1681.1986.tb02394.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARY1. The effect of substance P (SP) on pancreatic exocrine responses to exogenous cholecystokinin, secretin, and dopamine, were studied in the isolated and blood‐perfused pancreas of dogs.2. Intra‐arterial injection of SP had a significant biphasic effect on pancreatic secretion: an initial transient inhibition, followed by an increase in the secretion stimulated by the infusion of cholecystokinin. However, SP caused only an inhibition of secretion stimulated by the infusion of secretin and dopamine.3. SP increased protein concentration but not bicarbonate concentration in juice stimulated by cholecystokinin, but SP did not affect significantly either protein or bicarbonate concentrations in juice stimulated by secretin and dopamine.4. These results suggest that SP has greater effects on the pancreatic secretion stimulated by cholecystokinin than that stimulated by secretin and dopam

ISSN:0305-1870    

DOI:10.1111/j.1440-1681.1986.tb02395.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARY1. The effects of total and selective portasystemic shunting on hepatic haemodynamics and some aspects of liver function were studied in rats with dimethylnitro‐samine‐induced cirrhosis.2. Immediately following end‐to‐side portacaval shunting there were significant reductions in wedged hepatic venous pressure (WHVP) and liver blood flow. After side‐to‐side mesocaval shunting liver blood flow and wedged hepatic venous flow fell by approximately the same magnitude.3. Selective shunting (mesocaval ‘H’‐grafts and splenopancreaticocaval) preserved liver blood flow to a greater extent than total portasystemic shunting but had a less marked effect on WHVP.4. Furthermore, selective portasystemic shunting prevented liver atrophy and deterioration in liver function which was observed in rats following total portasystemic shunting.5. These results suggest that in the cirrhotic rat, selective portasystemic shunts which preserve functional liver blood flow and prevent liver atrophy and a deterioration in liver function do not produce such a marked decrease in WHVP as total shunts. Further studies in man are required to evaluate the relative advantages of total and selective por

ISSN:0305-1870    

DOI:10.1111/j.1440-1681.1986.tb02396.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARY1. Angiotensin converting enzyme (ACE) derived from rat lung, aorta, epididymus, brain, kidney and plasma was characterized by radio‐inhibitor (125I‐MK351A) binding studies. Under optimal binding conditions at equilibrium125I‐MK351A bound to ACE was displaced from ACE in a concentration related manner by unlabelled MK351A.2. MK351A binding site concentration for each tissue and equilibrium dissociation constant (KD) was estimated by Scatchard analysis of binding data. Binding sites/mg protein was greatest in lung and least in brain. The KDfor kidney ACE was significantly higher than that of lung, aorta, epididymus or brain ACE (P<0.005;t‐test, d.f. = 10).3.125I‐MK351A bound to ACE prepared from lung and kidney was displaced in a concentration dependent manner by SQ20881, SQ14225, MK422, and Ro31–3113–000. Concentration of ACE inhibitor required to displace 50% of bound125I‐MK351A (DD50) was consistently higher for kidney‐derived ACE than lung‐derived ACE.4. The differences in radio‐inhibitor binding characteristics of ACE from different rat tissues suggests that the enzyme active site may not be id

ISSN:0305-1870    

DOI:10.1111/j.1440-1681.1986.tb02397.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARY1. Intravenous administration to pithed Wistar rats of the angiotensin converting enzyme inhibitor enalapril (0.1–1.0 mg/kg) lowered the diastolic blood pressure and reduced pressor responses occurring during electrical stimulation (1–30 Hz) of the spinal sympathetic outflow.2. These doses of enalapril given intravenously also attenuated pressor responses to intravenous injection of the muscarinic ganglion stimulant McNeil‐A‐343 (50, 100, 150 μg/kg) and noradrenaline (0.1–5.0 μg/kg). Enalapril (1.0 mg/kg, i. v.) reduced pressor responses to the nicotinic ganglion stimulant 1,1‐dimethyl‐4‐phenylpiperazinium (300 μg/kg, i.v.).3. These results confirmed that the actions of enalapril resemble those of captopril in the pithed rat, by causing reductions in both blood pressure and pressor responses to s

ISSN:0305-1870    

DOI:10.1111/j.1440-1681.1986.tb02398.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

ISSN:0305-1870    

DOI:10.1111/j.1440-1681.1986.tb02399.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY