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1. |
The applications of hydrophobic interaction chromatography to the purification of plant proteins |
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Phytochemical Analysis,
Volume 2,
Issue 5,
1991,
Page 191-198
E. J. M. Pennings,
A. H. Meijer,
L. H. Stevens,
R. Verpoorte,
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摘要:
AbstractProtein separation by hydrophobic interaction chromatography (HIC) depends on the differences in surface hydrophobicity of the constituent proteins and is hence complementary to chromatographic techniques based on the charge or the size of the macromolecule. This review summarises some of the recent developments in HIC supports and discusses the parameters that must be considered in designing an efficient chromatographic system. The application of HIC to the purification of enzymes of secondary compound biosynthesis that cannot be obtained in pure form by size‐exclusion and ion‐exchange techniques alone is exemplified by reference to recent studies on tryptophan decarboxylase, strictosidine synthase and geraniol‐10‐hydr
ISSN:0958-0344
DOI:10.1002/pca.2800020502
出版商:John Wiley&Sons, Ltd.
年代:1991
数据来源: WILEY
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2. |
A bioautographic agar overlay method for the detection of antifungal compounds from higher plants |
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Phytochemical Analysis,
Volume 2,
Issue 5,
1991,
Page 199-203
L. Rahalison,
M. Hamburger,
K. Hostettmann,
M. Monod,
E. Frenk,
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摘要:
AbstractA simple bioautographic agar overlay assay usingCandida albicansas the indicator organism for the detection and activity‐guided fractionation of antifungal compounds by thin layer chromatography has been developed. Inhibition of fungal growth was assessed by the detection of dehydrogenase activity with thiazolyl blue (methylthiazolyltetrazolium chloride; MTT). A series of clinically used antimycotic agents were tested in order to determine the sensitivity of the assay. The compatibility of the agar overlay technique with chemically modified silica gel (Diol and RP‐18) plates and with various organic solvents was evaluated. The methodology is also applicable to the search for antibacterial compounds, as shown withBacillus subtilisas a test organ
ISSN:0958-0344
DOI:10.1002/pca.2800020503
出版商:John Wiley&Sons, Ltd.
年代:1991
数据来源: WILEY
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3. |
Determination of cyclic 3′,5′‐adenosine monophosphate in plant tissues by high performance liquid chromatography |
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Phytochemical Analysis,
Volume 2,
Issue 5,
1991,
Page 204-207
V. K. Yavorskaya,
I. V. Dragovoz,
S. V. Savinsky,
V. E. Markova,
I. Sh. Kofman,
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摘要:
AbstractA quantitative method has been developed for determining cyclic 3′,5′‐adenosine monophosphate (cAMP) in plant tissues based on the use of high performance liquid chromatography following partial purification of cyclonucleotides from plant extracts by different chromatographic procedures. The identical nature of a plant cAMP‐like substance and synthetic cAMP has been demonstrated. The lower limit of cAMP detection was 2.6 ng, the overall efficiency of determination was 34.0% and the standard deviation was ±5.2%. The concentration of cAMP in rye seedlings was determined to be 0.700±0.069 n mol per g fresh weight
ISSN:0958-0344
DOI:10.1002/pca.2800020504
出版商:John Wiley&Sons, Ltd.
年代:1991
数据来源: WILEY
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4. |
Simultaneous determination of atropine and scopolamine in plants by mixed‐column high performance liquid chromatography |
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Phytochemical Analysis,
Volume 2,
Issue 5,
1991,
Page 208-210
S. Mandal,
A. A. Naqvi,
R. S. Thakur,
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摘要:
AbstractThis paper describes a simple and rapid high performance liquid chromatographic method using two different polarity columns in series for the quantitative analysis of the major tropane alkaloids atropine and scopolamine. The problems associated with the simultaneous determination of the two alkaloids in plant extracts have been overcome. The method has been successfully applied to the analysis of three species of Solanaceous plants.
ISSN:0958-0344
DOI:10.1002/pca.2800020505
出版商:John Wiley&Sons, Ltd.
年代:1991
数据来源: WILEY
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5. |
Stereoisomeric flavour compounds. LII: Separation and structure elucidation of the furanoid linalool oxide stereoisomers using chirospecific capillary gas chromatography and nuclear magnetic resonance spectroscopy |
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Phytochemical Analysis,
Volume 2,
Issue 5,
1991,
Page 211-214
Christiane Askari,
Armin Mosandl,
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摘要:
AbstractUsing heptakis(2,3,6‐tri‐O‐methyl)‐β‐cyclodextrin as the chiral stationary phase in capillary gas chromatography, the direct stereodifferentiation of the four furanoid linalool oxide stereoisomers has been achieved. The structure elucidation of the two geometrical isomers by1H NMR spectroscopy (including intramolecular nuclear Overhauser effects of the separated diastereomers) is now reported. The elution order of the optical isomers is ascertained by synthesis and chromatography of the (2R)‐configured diastereomers, starting from
ISSN:0958-0344
DOI:10.1002/pca.2800020506
出版商:John Wiley&Sons, Ltd.
年代:1991
数据来源: WILEY
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6. |
Analysis of artemisinin and related sesquiterpenoids fromartemisia annuaL. by combined gas chromatography/mass spectrometry |
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Phytochemical Analysis,
Volume 2,
Issue 5,
1991,
Page 215-219
Herman J. Woerdenbag,
Niesko Pras,
Rein Bos,
Jan F. Visser,
Henk Hendriks,
Theo M. Malingré,
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摘要:
AbstractThe sesquiterpenoid artemisinin (3) and its biosynthetic precursors arteannuic acid (1), arteannuin B (2) and artemisitene (4) can be separated and identified by combined gas chromatography/mass spectrometry both as a mixture of reference standards as well as in extracts ofArtemisia annuaL. From this study a generally applicable gas chromatographic method has been developed for the analysis of these sesquiterpenoids inA. annua.Analysis of plant parts revealed that the highest sesquiterpenoid concentrations were present in the leaves, buds and small green stems, while the main stem contained only small amounts. Low levels of arteannuic acid and arteannuin B were present in the side‐roots. No sesquiterpenoids could be detected in the main root and none of the plant parts accumulated detectable quantities of artemisiten
ISSN:0958-0344
DOI:10.1002/pca.2800020507
出版商:John Wiley&Sons, Ltd.
年代:1991
数据来源: WILEY
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7. |
Separation of C25epimers of steroidal sapogenins by reversed phase high performance liquid chromatography at low temperature |
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Phytochemical Analysis,
Volume 2,
Issue 5,
1991,
Page 220-224
Yue Wu,
Peng Wu,
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摘要:
AbstractThree pairs of C25epimers of steroidal sapogenins–‐diosgenin (1) and yamogenin (2), hecogenin (3) and sisalagenin (4), tigogenin (5) and neotigogenin (6)–‐were separated by reversed phase high performance liquid chromatography. It is shown that the resolution of separation of the epimers increases with a decrease in column temperature and that optimal resolution is attained within the temperature range
ISSN:0958-0344
DOI:10.1002/pca.2800020508
出版商:John Wiley&Sons, Ltd.
年代:1991
数据来源: WILEY
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8. |
Quantitative determination by high performance liquid chromatography and microspectro‐fluorimetry of phenolic acids in maize grain |
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Phytochemical Analysis,
Volume 2,
Issue 5,
1991,
Page 225-229
A. Sen,
S. S. Miller,
J. T. Arnason,
R. G. Fulcher,
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摘要:
AbstractA method for improved recoveries of phenolic acids in maize grain extracts and their quantification by high performance liquid chromatography (HPLC) is described. A quantitative imaging technique to determine mean ferulic acid autofluorescence of ground grain samples was found to be correlated (r=0.86,n=14,P=0.05) with HPLC analysis of ferulic acid in a wide range of different maize genotypes. The advantage of the imaging method is that it reduces procedures required and analysis time considerably.
ISSN:0958-0344
DOI:10.1002/pca.2800020509
出版商:John Wiley&Sons, Ltd.
年代:1991
数据来源: WILEY
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9. |
Structure of petanin, an acylated anthocyanin isolated from solanum tuberosum, using homo‐and hetero‐nuclear two‐dimensional nuclear magnetic resonance techniques |
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Phytochemical Analysis,
Volume 2,
Issue 5,
1991,
Page 230-236
Øyvind M. Andersen,
Sigrid Opheim,
Dagfinn W. Aksnes,
Nils Åge Frøystein,
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摘要:
AbstractThe anthocyanin, petanin, was isolated from the acidified methanolic extracts of blue tubers of the potato (Solanum tuberosum) by successive application of ion exchange chromatography, droplet counter‐current chromatography and gel filtration. The full structure, petunidin 3‐O‐[6‐O‐(4‐O‐E‐p‐coumaroyl‐α‐L‐rhamnopyranosyl)‐β‐D‐glucopyranoside]‐5‐O‐β‐D‐glucopyranoside, with both the glucopyranose rings assuming a4C1chair conformation and the rhamnopyranose ring assuming a1C4chair conformation, was elucidated by means of chemical degradation, chromatography and spectroscopy, especially homo‐ and hetero‐nuclear two‐d
ISSN:0958-0344
DOI:10.1002/pca.2800020510
出版商:John Wiley&Sons, Ltd.
年代:1991
数据来源: WILEY
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10. |
Announcement |
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Phytochemical Analysis,
Volume 2,
Issue 5,
1991,
Page 237-237
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ISSN:0958-0344
DOI:10.1002/pca.2800020511
出版商:John Wiley&Sons, Ltd.
年代:1991
数据来源: WILEY
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