摘要:

AbstractThe possibilities and applications of capillary electrophoresis (CE) as an analytical technique for plant secondary metabolites are reviewed. Applications of both capillary zone electrophoresis and micellar electrokinetic capillary chromatography for this purpose are described. CE is compared with high pressure liquid chromato‐graphy (HPLC) as an analytical technique, and the advantages and criticisms of CE are described. The effects of the applied voltage, capillary temperature, electrolyte concentration and nature (complexing or non‐complexing buffers), buffer pH, micelle concentration and nature (sodium dodecyl sulphate or cetyltrimethylammonium bromide and the addition of organic modifiers to the running buffer (organic solvents, cyclodextrins, urea, cholate) on the parameters of separation and resolution of different secondary metabolites are discussed. The applications of CE to the analysis of flavonoid aglycones and glycosides, phenolic acids, quinones, coumarins, alkaloids, capsacinoids, glucosinolates, polyamines, monoterpenes, diterpenes, phytoecdysteroids, cardiac glycosides and saponins are shown. It is concluded that CE is a very promising analytical technique in the analysis of plant secondary metabolites, and it will become an indispensable tool, together with HPLC and gas liquid chromatography, in phytochemical laboratories since these techniques are in many ways complementary, and problems that are difficult to solve by HPLC can often be solved using

ISSN:0958-0344    

DOI:10.1002/pca.2800060402

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

摘要:

AbstractA capillary gas chromatographic method has been developed for the determination of canthinone alkaloids. The method was applied to the semi‐quantitative determination of canthin‐6‐one, 1‐methoxycanthin‐6‐one, 1‐hydroxycanthin‐6‐one, 8‐hydroxycanthin‐6‐one, 10‐hydroxycanthin‐6‐one, and canthin‐6‐one‐3‐N‐oxide in callus and shoot cultures, and in root barks ofHannoa chlorantha.The results showed that their concentrations were highly dependent on the samp

ISSN:0958-0344    

DOI:10.1002/pca.2800060403

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

摘要:

AbstractThe determination of six different isoquinoline alkaloids fromChelidonium majus(leaves and roots) using capillary electrophoresis is described. Analyses were performed on fused silica capillaries with 66 mM phosphate buffer at pH 2.4 containing 40% methanol. The analytes were monitored by on‐line ultraviolet (UV) detection to identify sanguinarine, chelerythrine, berberine and chelidonine. Both coptisine andDL‐stylopine were isolated by semi‐preparative high performance liquid chromatography (HPLC), and their structure assessed by UV and mass spectrometry. Capillary electrophoresis is confirmed as a useful complementary technique to

ISSN:0958-0344    

DOI:10.1002/pca.2800060404

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

摘要:

AbstractA protocol has been developed for the extraction and purification of solasodine from plant material to complement the use of benzoylation for derivatization of the compound prior to high performance liquid chromatographic (HPLC) determination. Solasodine glycosides were extracted in 80% ethanol, the samples defatted with chloroform and then hydrolysed with 0.5Mhydrochloric acid. The extracts were washed with ethyl acetate, prior to increasing the pH to between 8 and 9, whereafter solasodine was extracted with chloroform. Flash chromatography using a 15 cm silica gel column was necessary for purification of the extracts, which were then benzoylated and subjected to HPLC. In greenSolanum aculeastrumDun. berries, 0.016% solasodine was detected, whereas in callus cultures of the same species no solasodine was detectable.

ISSN:0958-0344    

DOI:10.1002/pca.2800060405

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

摘要:

AbstractPinocembrin was identified as the major compound responsible for the growth inhibition ofStaphylococcus aureusdisplayed by samples of propolis. The pinocembrin content of samples of propolis was determined by two chromatographic techniques, densitometry and high performance liquid chromatography. In both techniquess the pinocembrin content was assessed using internal standards. The pinocembrin content of samples of propolis from different temperate geographical areas was found to be in the range 4.0 to 4.6% w/w.

ISSN:0958-0344    

DOI:10.1002/pca.2800060406

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

摘要:

AbstractIsocratic and gradient elution high performance liquid chromatographic techniques are compared for separating different methylated and isoprenylated flavonols. The identification of individual flavonols from their isomers, based on retention times, thin layer chromatographic data and ultraviolet spectroscopy is discussed. These data are provided for 23 kaempferol, quercetin and myricetinO‐methyl ethers, for sevenC‐methyl ethers and 15 isoprenylated derivati

ISSN:0958-0344    

DOI:10.1002/pca.2800060407

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

摘要:

AbstractUnderivatized alkylbutenolides from severalPeucedanumspecies includingP. alsaticumL.,P. austriacum(Jacq.) Koch,P. arenariumWaldst.&Kit.,P. venetum(Sprengel) Koch andP. rablense(Wulfen) Koch (Apiaceae‐Apioideae) were separated by high performance liquid chromatography and preparative chromatography on a reversed phase system at a pH of 2.5. The chromatographic separation on RP‐18 with methanol: water as eluent is strongly affected by the enol‐enolate equilibrium of these compounds. In protic solutions these compounds exist as enolate anions. The dissociation constant of alkylbutenolides in methanol‐:water mixtures was determined. The alkylbutenolides show rather good solubility in polar solvents and high acidity compared to the corresponding fatt

ISSN:0958-0344    

DOI:10.1002/pca.2800060408

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

ISSN:0958-0344    

DOI:10.1002/pca.2800060409

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

ISSN:0958-0344    

DOI:10.1002/pca.2800060410

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

ISSN:0958-0344    

DOI:10.1002/pca.2800060401

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY