ISSN:0958-0344    

DOI:10.1002/pca.2800040202

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractThis paper describes the estimation of triacylglycerol (TAG) in wheat embryos by a method based on the determination of glycerol. Modifications have been made to the method currently in use for serum TAGs to make it suitable for application with plant tissues, particularly the substitution of a chemical hydrolysis of TAG in place of the enzymatic lipolysis. The method is relatively rapid, highly reproducible and gives results which are in close agreement to those obtained by analysis of the fatty acid methyl esters. There is no interference by other glycerol‐containing compounds such as phospholipids or glycerol phosphates. Examples are given of the method applied to wheat embryos accumulating TAGin plantaor treatedin vitroin different way

ISSN:0958-0344    

DOI:10.1002/pca.2800040203

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractIon‐pair and ion‐suppression mode high performance liquid chromatography was conducted on the alkaloidal constituents of the roots ofArtabotrys monteiroae, an important medicinal plant in Kenya having analgesic and anti‐inflammatory action. Use of sodium perchlorate as an ion‐pair reagent led to excellent separation. In the ion‐suppression system containing ammonium acetate, the separation was rather poor, but liquid chromatography‐mass spectrometry was possible, which was not the case in the ion‐pair system. Four aporphines, (‐)‐asimilobine, (+)‐wilsonirine, (+)‐norisoboldine and anonaine, and a benzylisoquinoline, (+)‐reticuline, were identified after isolation by preparative th

ISSN:0958-0344    

DOI:10.1002/pca.2800040204

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractThe isolation and purification of velutinol A, a potent anti‐inflammatory agent fromMandevilla velutinais reported together with spectroscopic evidence which shows that the structure comprises a novel pregnane skeleto

ISSN:0958-0344    

DOI:10.1002/pca.2800040205

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractPhytoalexins are secondary metabolites that in legumes are typically isoflavonoid derivatives. Techniques for their measurement very often use determination of the UV absorbance of ethanol extracts to avoid time‐consuming extractions and the use of expensive solvents. However, other phenolic compounds also give UV absorbances interfering with quantitative determinations. A microassay is proposed to extract and measure bean phytoalexins from intact, viable, bean leaf protoplasts. Protoplasts as a tool for measuring phytoalexin elicitor stimulation is discusse

ISSN:0958-0344    

DOI:10.1002/pca.2800040206

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractA reverse‐phase high pressure liquid chromatographic determination of 11 hydroxycoumarins in plant extracts is described, which is applicable to the quantitative analysis of these compounds for industrial purposes.Fraxinus ornusbark from various regions in Bulgaria showed differences both in the total coumarin content and in the relative proportions of each component. The occurrence of 7‐methylesculin and 6,7,8‐trihydroxycoumarin in the family Oleaceae is reported for the first

ISSN:0958-0344    

DOI:10.1002/pca.2800040207

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

ISSN:0958-0344    

DOI:10.1002/pca.2800040208

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

ISSN:0958-0344    

DOI:10.1002/pca.2800040209

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

ISSN:0958-0344    

DOI:10.1002/pca.2800040201

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY