摘要:

Inbred DBA2 mice bearing the syngeneic 1699 mammary tumour in the hind limb were challenged intravenously with 125IUdR-labelled single 1699 cell suspensions, and the amount of radioactivity in the lungs compared 20–24 h later with that in the lungs of normal mice or in those of mice bearing the antigenically unrelated syngeneic SaD2 tumour. An immunologically specific decrease in radioactivity was evident at variable periods of time after tumour induction, depending on the number of cells used to induce the leg tumours, but fell below that in normal mice as the leg tumours progressed beyond a weight of approximately 1 g. As assessed by microscopic scanning of serial histological sections of the same lungs the incidence of spontaneous metastases rose to between 80 and 100% immediately the amount of cell loss from the lungs of the tumour-bearing mice reached that of the normal controls. This extremely rapid series of events does not allow a definitive conclusion as to whether immunity failed and led to metastatic spread or vice versa, but does underline the strong association of immunity with the blood-borne dissemination of tumour cells in this tumour system. Following excision of tumours, in no instance was immunity detected 14 days later, and in a single experiment did not reach detectable limits until 25 days after excision, at a time when the lung metastases were observed mostly to have regressed spontaneousl

ISSN:0030-2414    

DOI:10.1159/000225318

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

Some cell cultures synthesize a mitogenic factor (DNASF) following their lytic infection with either HSV-1 or HSV-2 which is shed into the medium. Of six cell lines permissive to HSV infection tested, three produce DNASF (CV1, HF, MCA) and a significantly higher (3H)-TdR incorporation was obtained with indicator cells growing in supernatants of virus-infected cells (SupV+) than in the corresponding supernatants of sham-treated cells (SupV––). Sometimes the values obtained with SupV+ exceed those obtained with cell controls, which demonstrates that DNASF is not simply a nutritional factor. Other cell lines do not produce DNASF (HeLa, Wistar and probably REF) and SupV+ frequently inhibit cell growth. DNASF is genetically nonspecific and all indicator cells tested were stimulated by supernatants which contain the mitogen, including the nonpermissive DC-3F cells. The quantity of DNASF induced is directly proportional to the number of cells in the culture and its production peaks when the lysis of the cells is complete. It is not accumulated inside the ce

ISSN:0030-2414    

DOI:10.1159/000225319

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

The respiratory tract of Syrian golden hamsters was exposed to polycyclic hydrocarbons alone and in combination with dusts. These included benzo(a)pyrene (B(a)P), silicon dioxide (SiO2), manganese dioxide (MnO2) and agar gelatin (gel), as well as dibenz(a, j)acridine (DBA) in combination with the carrier dust ferric oxide (Fe2O3). The purpose of the study was to evaluate the possible environmental hazards of these compounds and the physicochemical characteristics determining their carcinogenic activity. We also wished to study the prognostic significance of premalignant alterations in relation to treatment by carcinogenic and noncarcinogenic agents and the applicability of respiratory tumor classification in this system. When instilled alone, the dusts induced bronchial nonspecific epithelial alterations, interstitial cell proliferation and a few granulomatous changes in the lung. No respiratory tract tumors were seen. B(a)P alone induced only tracheal epithelial alterations, desquamation and metaplasia, as well as five papillomas and one squamous cell carcinoma. B(a)P in a suspension of gelatin-saline induced a few papillomas and squamous cell carcinomas of the trachea and lungs. SiO2 with B(a)P was highly effective and induced papillomas, squamous cell carcinomas, adenomas and adenocarcinomas of the larynx, trachea and lungs. They were preceded by epithelial proliferative dysplastic alterations, not seen in animals treated with B(a)P and MnO2 or DBA and Fe2O3. These groups also failed to exhibit a significant neoplastic response. The tumors could be divided into benign and malignant, glandular or squamous cell tumors. The trachea, main bronchi, bronchioli and lungs showed the efficacy and applicability of a classification similar to that used in man. It is also concluded that preneoplastic alterations sufficient in degree and extent are indicative of subsequent tumor formation.

ISSN:0030-2414    

DOI:10.1159/000225320

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

The present communication reports data on the lipid biosynthesis of Ehrlich ascites tumor cells grown in culture media supplemented with modified sera. Whereas the metabolisms of [14C] pyruvate and [14C] mevalonate are identical in all media tested, the incorporation of [14C] acetate is higher in medium with dialyzed serum than in medium with delipidized serum; it is suppressed in the absence of all lipids in culture medium. Cellular integrity is not impaired in modified media. The results indicate that acetyl-CoA synthetase of Ehrlich ascites tumor cells is not regulated by exogenous lipids as is known to be the case in nonmalignant cells.

ISSN:0030-2414    

DOI:10.1159/000225321

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

Mitomycin C (MMC) induced chromosome aberrations and an increased sister chromatid exchange (SCE) incidence in a cell line derived from normal lymphocytes, whereas in lines originating from cells of chronic lymphocytic leukemia (lines SN1029 and SN1033) and Burkitt lymphoma (lines B35M and HR1K) MMC caused a striking increase in SCE, but not in the frequency of chromosome abnormalities. The latter may be due to the failure of the cells with chromosome aberrations to survive, possibly related to their high sensitivity to MMC. The increased rate of SCE with MMC treatment was more marked in the neoplastic lymphocytic cells than in the normal ones; the sensitivity of SN1029 and SN1033 cells was 10 times higher and that of B35M and HR1K cells about 5 times higher than that of the normal cells. These observations suggest that the MMC-induced high rate of SCE in the neoplastic cells may in some aspects differ from the mechanism(s) leading to chromosome aberrations; and that SCE is a much more sensitive indicator of MMC effects than chromosome aberrations.

ISSN:0030-2414    

DOI:10.1159/000225322

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

TA3/Ha and TA3/St sublines differ from each other in some characteristics, like malignancy, survival time and drug sensitivity. TA3/St exhibited a peculiar response to the Vinca derivatives examined.

ISSN:0030-2414    

DOI:10.1159/000225323

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

Solitary myeloma is an uncommon lesion which may occur in many organs, mainly in the rhinopharynx. This lesion usually appears as non-secretory, and it is constituted by either typical plasma cells or large and atypical cells resembling epithelial anaplastic cells. Therefore, an immunofluorescent method on paraffin-embedded sections may be applied to reveal the presence and the monoclonality of the intracytoplasmic Ig in the pathological cells. In this paper we report a study on 22 patients suspected to bear a rhinopharyngeal tumor investigated by immunofluorescence. Moreover, in 4 cases peripheral blood lymphocytes were also studied. The results are discussed on the basis of the modern immunological trends.

ISSN:0030-2414    

DOI:10.1159/000225324

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

A comparison of bone scintigrams and roentgenographic skeletal surveys, obtained on 170 patients with breast carcinoma, was made to evaluate the diagnostic efficacy of these techniques in detecting metastatic bone lesions. The bone scans were abnormal in 81 patients, while the roentgenograms were abnormal in only 51. In 34 of the 81 (42%) patients with abnormal bone scans, there was no radiographic evidence of a benign lesion to account for the increased ratiotracer uptake; and the abnormalities noted on the bone scans were proven to be bony metastases on follow-up examinations. In the remaining 47 patients with positive bone scintigrams, both the scans and the roentgenograms were abnormal; however, in 23 patients the bone scans demonstrated significantly more lesions than what the roentgenograms had revealed. This study confirms that bone scan is superior to roentgen surveys in detecting skeletal metastases in patients with breast carcinoma. It was noted that the metastatic lesions can be visualized on the scans earlier than they are apparent on the X-rays by a mean interval of 4 months.

ISSN:0030-2414    

DOI:10.1159/000225325

出版商:S. Karger AG    

年代:1979

数据来源: Karger

ISSN:0030-2414    

DOI:10.1159/000225326

出版商:S. Karger AG    

年代:1979

数据来源: Karger