摘要:

The heterogeneity of CMV DNA obtained from standard strains and new isolates, including a vaccination strain (Towne 125), was investigated. The cleavage patterns produced by the restriction endonucleases Eco RI and Bam 1 revealed stable strain specificities of CMV. On the other hand, a remarkable homology of sequence-specific CMV DNA fragmentation was demonstrated. A CMV subtyping relevant to clinical questions seems to be improbable.

ISSN:0030-2414    

DOI:10.1159/000225352

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

Explant cultures from several human prostatic tissues have been examined for oncornavirus production. Some of these explants, epithelial in morphology, appear to release oncornavirus-like particles. The extracellular particulate material, obtained from the culture medium of these explants display the following characteristics: (1) It contains particles that band at a density of 1.1–1.2 g/cm3 in a sucrose density gradient. (2) The particles contain RNA-directed DNA polymerase. This polymerase utilizes poly(Cm) as a template and shows preference for poly(A) over poly(dA) and manganese over magnesium. (3) Apparently, the particles also contain RNA that directs the synthesis of DNA in vitro. The DNA thus synthesized is associated with RNA, some of it with high molecular weight RNA. Such particles are not observed in the culture medium from monolayers of monkey kidney CV-1 cells. We have found that explant cultures from 5 out of 15 tissue specimens with prostatic hyperplasia and 3 out of 4 with prostatic adenocarcinoma release particles that band at a density of 1.1–1.2 g/cm3 and contain RNA-directed DNA polymerase. Explant cultures of two normal prostates examined did not release such partic

ISSN:0030-2414    

DOI:10.1159/000225353

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

In this study we demonstrated that γG2A myeloma cells in syngeneic BALB/c mice and Ehrlich adenocarcinoma cells in outbred Swiss mice, when grown in animals previously immunized against RRBC, have RRBC antibodies bound on their surface. Ascites tumor cells obtained from animals not immunized against RRBC can bind RRBC antibodies from the medium. Tumor cells from RRBC-immunized mice and tumor cells withRRBC antibodies bound in vitro were both able to form ‘rosettes’ when tested with RRBC, thus demonstrating that the combining site of the antibody molecule was not involved in the binding. Some theoretical implications of the presence of nonspecific antitumor immunoglobulins on cancer cell membrane are discu

ISSN:0030-2414    

DOI:10.1159/000225354

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

Total lactic dehydrogenase (LDH) levels and LDH isozyme patterns were measured in homogenates of 12 autochthonous ethylnitrosourea-induced rat gliomas and 5 cloned rat astrocytoma cell lines maintained in culture and transplanted to brain or flank sites in syngeneic hosts. The total LDH values in the autochthonous gliomas did not differ appreciably from normal brain controls, but the proportions of the cathodal isozymes, LDH4 and LDH5, were increased to a degree similar to that reported by others in spontaneous human malignant astrocytic gliomas. The cloned astrocytoma lines, both in vitro and in transplants at intracerebral or subcutaneous sites, commonly demonstrated elevated total LDH values and, without exception, showed a preponderance of isozymes, LDH4 and LDH5, that was distinctly more marked than in autochthonous tumors. Especially in cultured and transplanted rat gliomas, these findings suggest that astrocytic tumor cells maintain energy supplies by utilizing anaerobic glycolysis in relatively hypoxic environments. These data further underscore the need to develop laboratory brain tumor models for use in therapy trials that not only retain the convenience and predictability of transplantable gliomas but also approximate closely the metabolic properties of human spontaneous gliomas.

ISSN:0030-2414    

DOI:10.1159/000225355

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

Antibodies were raised in rabbits against pure ligandin. Employing the fluorescent antibody procedure, an antigen identical to ligandin was shown to be uniformly distributed in the hepatic cells of both normal rat liver and in early and late 3′-methyl-4-dimethylaminoazobenzene (3′-Me-DAB)-treated liver. Ligandin was absent in liver carcinomas induced by this azocarcinogen. The results obtained with the fluorescent antibody procedure were confirmed by the immunoquantitation of ligandin in the cytosols prepared from normal rat liver, early and late 3′-Me-DAB-treated liver, mixtures of both late 3′-Me-DAB-treated liver and liver carcinomas, and liver carcinomas. Riboflavin-deficient diets lowered significantly the hepatic ligandin

ISSN:0030-2414    

DOI:10.1159/000225356

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

Further biochemical characterization of the Albert hepatoma has been performed. The following results were obtained: In spite of often repeated transplantations and a medium growth rate, the Albert hepatoma does still contain organ-characteristic enzymes. We have found significant activities of glucokinase, glucose-6-phosphatase and arginase, and considerable amounts of noradrenaline and glycogen. In addition, it is capable to respond to humoral stimuli, that is, it differs from most of the other hepatocellular malignomas also in this regard.

ISSN:0030-2414    

DOI:10.1159/000225357

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

Bovine pineal polypeptide extract (PPE) exerted an anti-tumor effect on mouse-transplantable tumors: mammary cancer (RSM), squamous cell cervical carcinoma (SCC), hepatoma-22a and lympholeuke-mia LIO-1, and had no effect on Harding-Passey melanoma and leukemia L-1210. It was shown that PPE possessed the ability to decrease the incidence of DMBA-induced mammary adenocarcinomas in rats. The daily administration of 0.5 mg PPE prolonged the life span of rats by 25% and failed to influence spontaneous tumor development. The arguments in favor of a possible mechanism of anti-tumor action of the pineal gland are submitted. It is suggested that the anti-tumor effect of PPE may occur when the syndrome of cancrophilia is induced by tumor transplantation or chemical carcinogens.

ISSN:0030-2414    

DOI:10.1159/000225358

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

A unique case of multiple keratoacanthomas is described, with involvement of the face and lower extremities with persistent lesions clinically and histologically diagnostic of keratoacanthomas. The lower extremity lesions appear as giant keratoacanthomas, with evolution into a distinctive confluent plaque. This important tumor and its present classification are reviewed.

ISSN:0030-2414    

DOI:10.1159/000225359

出版商:S. Karger AG    

年代:1979

数据来源: Karger

ISSN:0030-2414    

DOI:10.1159/000225360

出版商:S. Karger AG    

年代:1979

数据来源: Karger

ISSN:0030-2414    

DOI:10.1159/000225361

出版商:S. Karger AG    

年代:1979

数据来源: Karger