摘要:

200 male Wistar rats were transplanted subcutaneously with Walker tumour. After 1 month, the tumour was surgically removed and, after an other 5 months, the histological pattern of the thymus of 12 rats which exhibited no recurrence, was studied. An almost complete recovery of the thymus of these animals was observed in comparison with controls of the same age. Some histological imprints of the transient involution undergone by the thymus during the first month, may be, nevertheless, identified.

ISSN:0030-2414    

DOI:10.1159/000224741

出版商:S. Karger AG    

年代:1973

数据来源: Karger

摘要:

Cervical carcinoma in situ is an ideal human research model for experimental immunotherapy because of slow evolution over several years and because spontaneous regression is rare if treatment is withheld. Exfoliative cytology provides a means of precision control to assure safety and to evaluate cell changes compared before, during and after treatment. This long-term report on a small but significant group of 14 cervical cancers (stage 0) treated with a coupled tumor protein antigen produced some regressive cell alterations in all 14 patients and total regression in 5. None of those treated showed progression and there was no evidence of toxicity to kidneys, liver or blood observed. Lymphocytosis (30–60%) was noted during tumor antigen administration. Photomicrographs illustrating the manifestations of cell-mediated immune reaction demonstrate possible mechanisms of tumor cell regressio

ISSN:0030-2414    

DOI:10.1159/000224742

出版商:S. Karger AG    

年代:1973

数据来源: Karger

摘要:

Morphological and cytogenetical studies of H10Z tumor in various periods of growth in animals emphasize its heterogenous constitution formed by two groups of cell types, i.e. (1) ‘primary cell types’, the main constituents of tumor structure, well defined morphologically and genetically, which are gradually selected during tumor evolution; (2) ‘secondary cell types’, which are distinguished only on genetical analysis by the presence of certain new marker chromosomes. These cells are very scarce in the tumor and their distribution does not present significant variations during tumor growth. The conclusion is drawn that modification of tumor characteristics during its evolution in animals takes place by gradual selection of certain primary cell types. The possibility of tumor variant appearance in special medium conditions, by the occurrence of the induction-selection phenomenon in a secondary cell type, is di

ISSN:0030-2414    

DOI:10.1159/000224743

出版商:S. Karger AG    

年代:1973

数据来源: Karger

摘要:

Tumor-specific antisera were prepared by immunizing rabbits with a non-particulate fraction of 7,12-dimethylbenz(a)anthracene-induced rat mammary tumors. After extensive absorption with lyophilized rat serum and organ preparations, the removal of precipitating antibodies to ‘normal’ antigens was confirmed by double-immuno-diffusion in agar gels and Immunoelectrophoresis. The antiserum was conjugated with fluorescein isothiocyanate and sections from mammary tumors and normal organs were treated with this preparation. The sections were examined by darkground fluorescent microscopy and the specificity of the immuno-fluorescent stain was established by its limitation to tumor specim

ISSN:0030-2414    

DOI:10.1159/000224744

出版商:S. Karger AG    

年代:1973

数据来源: Karger

摘要:

The soluble cytoplasmic proteins isolated from bronchial mucosae and 15 bronchogenic carcinomas of human origin have been examined by agarose-gel electrophoresis and compared with a similar protein preparation isolated from the bronchial mucosa of cynomolgus monkeys (Macaca irus). In all cases, 3 bands were found, 2 (A and B) which migrated to the anode and one (C) which migrated to the cathode. The relative proportions of protein in the 3 bands were similar in smokers and nonsmokers, but there was a significant increase in the proportion of protein in band C in all of the bronchogenic carcinoma material examined. This increase was most pronounced in ‘oat’-cell carcinomas. The finding that basic proteins are increased in bronchogenic carcinoma is in contrast with the decrease in basic proteins in some experimental animal tumors; it is suggested that this difference might be related to functional differences between basic proteins in different tissues. The electrophoretic pattern of human and cynomolgus monkey material was similar, indicating that the cynomolgus monkey may be a suitable model for gaining experimental data of relevance to alterations in these proteins in human mater

ISSN:0030-2414    

DOI:10.1159/000224745

出版商:S. Karger AG    

年代:1973

数据来源: Karger

摘要:

The observations described here were made partly on cultured human amnion cells (AV3/INO clone cell line) and partly on mouse embryo cells transformed into neoplastic cells in vitro by SV40 virus (ER-SV40 cells). The procedures designed by us for cell cloning and for adaptation to monodisperse suspension cultures are described. The cell cycle of AV3/INO cells was studied by pulse-labeling with tritiated thymidine. An interesting phenomenon, a very peculiar and probably significant intracell formation is described. Intracells are classified as either spurious, i.e. cells incorporated into another cell by phagocytosis, or genuine, formed within the mother cell by a gemmation process of the endoplasmic reticulum, with subsequent organization of a nucleolus and nucleus. The gemmule may be formed in two ways, which are termed prevacuolar or disjunctive, and postvacuolar. The DNA of the nucleus of intracells could originate either from the nucleus or from the mitochondria of the mother cell.

ISSN:0030-2414    

DOI:10.1159/000224746

出版商:S. Karger AG    

年代:1973

数据来源: Karger

摘要:

In Planarians, dedifferentiation leads to the production of syncytial elements which, in the intercellular spaces of the parenchyma, are in the place of the ground substance of other Metazoans. Syncytial elements are not found in intact and normally fed Planarians; they are readily observed in animals previously starved or having undergone several successive regenerations. These conditions provoke a massive dedifferentiation of cells whose plasma membranes disappear and whose endoplasmic reticulum is resorbed while the number of mitochondria is considerably reduced. After completion of dedifferentiation, the syncytium’s fundamental hyaloplasm abounds in ribosomes and is practically without membranes, except in the immediate vicinity of the nuclei. At the base of the regenerate this syncytium divides into very regular cell territories, as a result of the appearance of thin plasma membranes in the hyaloplasm; these membranes have no apparent connection with the remains of the endoplasmic reticulum. The cells, which certain researchers refer to as ‘neoblasts’ and which are observable by optic microscopy, undergo the same dedifferentiation phenomena as the others during regeneration; this is in accordance with the glandular nature that other researchers have attributed to these

ISSN:0030-2414    

DOI:10.1159/000224747

出版商:S. Karger AG    

年代:1973

数据来源: Karger