摘要:

ABSTRACTThe effect of sera from patients with liver cirrhosis (LC) and from healthy donors on natural killer (NK) activity of normal peripheral blood mononuclear cells (PBMC) was investigated. PBMC from a healthy donor were pre‐incubated for 3 h at 37°C in media containing 50% test sera and washed twice with phosphate‐buffered saline (PBS). Cytotoxic activity was determined using51Cr‐labelled K‐562 cells as target. It was demonstrated that sera from both healthy donors and patients with LC suppressed the NK activity of normal PBMC compared with a PBS control. But the level of inhibition caused by patients’ sera (n= 30) was significantly higher than that caused by normal sera (n= 30;P<0.001, Wilcoxon rank sum test). Sera from a patient with LC and a healthy subject were fractionated by a Sephacryl S‐300 column and the effect of five fractions on NK activity of normal MNC was assessed. Fractions 3 and 4 from both sera had inhibitory effects although Fraction 5 revealed an enhancing effect. These results suggest that human sera contain factors which both inhibit and enhance NK activity and that their net effects are inhibition. Since it is known that IgG is eluted in Fraction 4, IgG was separated from sera with a DEAE‐Sephacel column and its effect on NK activity was examined. IgG from patients’ sera was observed to suppress NK activity more often than those from healthy donors at the sam

ISSN:0815-9319    

DOI:10.1111/j.1440-1746.1986.tb00774.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

ABSTRACTAnin vivo in situliver preparation in anaesthetized male Sprague‐Dawley rats was devised for simultaneous measurements of hepatic microvascular flow and bile flow. The micro‐vascular flow on the surface of the liver was continuously recorded by laser Doppler flowmetric technique whilst bile flow was quantitated by connecting the bile duct cannula to a drop counter. Sodium dehydrocholate was infused (0.2 ml in 2–5 min) both via a cannula in the splenic artery and via a cannula in the superior mesenteric vein. Results showed that sodium dehydrocholate, given by either route, increased the liver microvascular flow and bile flow. A cross‐plot between the hyperaemic and hypercholeretic responses corresponding to the same dose of sodium dehydrocholate revealed a significant correlation (P<0.01) between the sodium dehydro‐cholate‐induced hypercholeresis and hyperaemia. Furthermore, the increase of microvascular flow occurred significantly (P<0.05) earlier than the hypercholeretic response. Results thus suggested that an increase of plasma blood flow was essential for the development of hypercholeresis induced by sodium deh

ISSN:0815-9319    

DOI:10.1111/j.1440-1746.1986.tb00775.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

ABSTRACTThis report presents the clinical features of 78 cases of the chronic Budd‐Chiari syndrome encountered over a period of 13 years. The diagnosis of hepatic venous outflow obstruction was confirmed by venographic studies in all cases. In 20 patients there was hepatic vein occlusion without inferior vena caval (IVC) obstruction (Group A). In 17 patients there was constriction of the IVC above the drainage site of the right hepatic vein which was patent (Group B). In 13 patients there was short segmental obstruction of the hepatic segment of the IVC along with blockage of the hepatic venous orifices (Group C). In 28 patients there was hepatic venous obstruction with long segment involvement of the IVC extending to varying lengths of the infrahepatic segment (Group D). Of particular interest are the operative findings in 12 of 17 patients of Group B of hour glass constriction of the IVC, which can be labelled as ‘coarctation of the IVC'. Dorsal cavoatrial bypass using a polytetra fluoroethylene graft has proved useful in Group B. Interesting histopathological findings of the liver in some of the cases are also described. The possible aetiology of the Group B cases is discus

ISSN:0815-9319    

DOI:10.1111/j.1440-1746.1986.tb00776.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

ABSTRACTAuto‐antibodies including antinuclear antibody (ANA), antismooth muscle antibody, antimitochondrial antibody, rheumatoid factor, lupus erythematosus factor, antimicrosomal antibody and antithyroglobulin antibody were assayed in sera from 84 patients with hepatocellular carcinoma, 70 with liver cirrhosis, 50 with chronic hepatitis, 30 with cancer of the alimentary tract and 100 normal subjects. A significantly higher incidence and higher titre of ANA was found in patients with hepatocellular carcinoma, and the patterns of nuclear fluorescence detected by the indirect immunofluorescent test using cultured baby hamster kidney cells were predominantly speckled and nucleolar. In 16 patients with this disease, serial assays of ANA were done in sera obtained before and after development of hepatoma and/or after resection of the hepatic tumour. Antinuclear antibodies evolved in six patients in conjunction with the progression from cirrhosis to hepatoma, and two of three ANA‐positive patients who had the hepatic tumour resected lost ANA from their sera after resection.ABSTRACTThe significance of ANA in the sera of patients with malignant neoplasms is yet to be clarified, but it may be closely associated with the evolution of hepatocellular carcin

ISSN:0815-9319    

DOI:10.1111/j.1440-1746.1986.tb00777.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

ABSTRACTThe simultaneous administration of diisopropyl, 1,3‐dithiol‐2‐ylidinemalonate (malotilate) suppressed the proliferation of AFP‐positive oval cells, the formation of hyperplastic nodules and the appearance of clusters of AFP‐positive cells in rat liver with hyperplastic nodules in 3′‐methyl‐4‐dimethylaminoazobenzene (3′‐Me‐DAB) hepatocarcinogenesis. The development of hepatocarcinoma, induced by 3′‐Me‐DAB, was delayed in rats treated with malotilate; therefore, the simultaneous administration of malotilate worked as an inhibitory effect on 3

ISSN:0815-9319    

DOI:10.1111/j.1440-1746.1986.tb00778.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

ABSTRACTRadionuclide angiography was used to generate curves of first‐pass radioactivity vs. time for the right hepatic lobe and both kidneys following the rapid intravenous injection of 10 mCi of99mTc‐phytate. By analysis of the right hepatic curve, the arterial and portal components of the liver circulation were calculated in 21 healthy subjects and 183 patients, 39 with chronic hepatitis and 144 with cirrhosis of the liver.ABSTRACTIn the healthy subjects, the portal component (PC) of liver blood supply ranged from 64% to 79% (mean 71.5%). In patients with hepatitis the mean PC was 61.3%, and in patients with cirrhosis it was 37.9%. The PC was lower in cirrhotic patients with oesophageal varices than in those without (P<0.001).ABSTRACTThe PC was assessed in 23 of the patients with cirrhosis before and after sclerotherapy for oesophageal varices. A pairedt‐test showed significant rises in this component immediately after the final treatment (P<0.001). Two months after this treatment, the mean PC had dropped significantly, approaching the pre‐treatment value (P<0.001). One year after treatment, the PC had not dropped further.ABSTRACTThese findings suggest that this non‐invasive method may be useful for the assessment of portal circulatory changes in cirrhosis, and of results of sclerotherapy for oesophagea

ISSN:0815-9319    

DOI:10.1111/j.1440-1746.1986.tb00779.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

ABSTRACTA 4‐week placebo‐controlled trial was performed to study the efficacy of sulpiride, a hypothalamic non‐sedative neuroleptic and dopamine antagonist, in the treatment of 100 patients with functional dyspepsia, defined as dyspeptic symptoms despite normal endoscopy and cholecystography. Two patients on sulpiride were withdrawn because of sleepiness; no other undue side effects were recorded. At the end of 4 weeks, significantly (P<0.02) more patients on sulpiride had improvement of nausea and belching (78%, 54% respectively) than patients on placebo (45%, 17% respectively). Pain and vomiting disappeared in approximately 50% and 70% of patients, respectively. In the overall assessment, 72% and 39% of the patients on sulpiride and placebo respectively had satisfactory improvement or became symptom‐free (P<0.001). Response to treatment was not related to personal and environmental factors. It was concluded that sulpiride was effective in improving symptoms of dyspepsia, particularly nausea and b

ISSN:0815-9319    

DOI:10.1111/j.1440-1746.1986.tb00780.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

ABSTRACTIn recent years clinical interest in the study of the proteolytic enzymes of the stomach has greatly increased. Human pepsinogens belong to the group of aspartic proteases and are categorized into two main groups: Pepsinogen A (PGA = PG I) and Pepsinogen C (PGC = PG II). Genetic models have been proposed to explain the inheritance of PGA, and a recent multigene model may be of value. PGA phenotypes in urine and gastric mucosa have been determined in healthy volunteers as well as in patients with different gastric disorders. An increased frequency of the ‘intense Pg5’ phenotype seems to be associated with gastric cancer and pre‐malignant conditions, such as atrophic gastritis. Reliable radio‐immunoassay and enzyme‐linked immunosorbent assay techniques have facilitated the study of serum levels of PGA and PGC in different patient groups; in particular, duodenal ulcer patients (high PGA levels) and patients with atrophic gastritis and/or gastric cancer (low PGA levels). The ratio serum PGA/PGC may be introduced for clinical application, being the most convenient non‐invasive marker for the detection of fundic atrophy. While the chromosome localization of pepsinogen has been established, further research is likely to concentrate on the structure and organization of the pepsinogen genes at the DNA‐level, as well as on the development of new isozyme specific monoclon

ISSN:0815-9319    

DOI:10.1111/j.1440-1746.1986.tb00781.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

ISSN:0815-9319    

DOI:10.1111/j.1440-1746.1986.tb00782.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

ISSN:0815-9319    

DOI:10.1111/j.1440-1746.1986.tb00783.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY